Fluorescence microscopy characterization

Gurrieri, S. Wells, K. S. Johnson, 1. D. Bustamante, C. Direct visualization of individual DNA molecules by fluorescence microscopy characterization of the factors affecting signal/background and optimization of imaging conditions using YOYO. Anal. Biochem. 1997,249,44-53. 

The synthesis and characterization of a somatostatin receptor-specific peptide H2N-(DPhe)-cyclo[Cys-Phe-(D-Trp)-Lys-Thr-Cys]-Thr-OH, labeled with an indo-dicarbo- and an indotricarbocyanine dye at the V-terminal amino group were described in [34], The ability of these fluorescent contrast agents to target the somatostatin receptor was demonstrated by flow cytometry in vitro, wherein the indotricarbocyanine conjugate led to elevated cell-associated fluorescence on somatostatin receptor-expressing tumor cells. The intracellular localization was visualized using NIR fluorescence microscopy. 

Plant Cells and Tissues Structure-Function Relationships. Methods for the Cytochemical/Histochemical Localization of Plant Cell/Tissue Chemicals. Methods in Light Microscope Radioautography. Some Fluorescence Microscopical Methods for Use with Algal, Fungal, and Plant Cells. Fluorescence Microscopy of Aniline Blue Stained Pistils. A Short Introduction to Immunocytochemistry and a Protocol for Immunovi-sualization of Proteins with Alkaline Phosphatase. The Fixation of Chemical Forms on Nitrocellulose Membranes. Dark-Field Microscopy and Its Application to Pollen Tube Culture. Computer-Assisted Microphotometry. Isolation and Characterization of... 

Gilman JW, Davis RD, BeUayer S et al. (2005) Use of optical probes and laser scanning con-focal fluorescence microscopy for high-throughput characterization of dispersion in polymer layered silicate nanocomposites. PMSE Prepr 92 168-169... 

During the next lew years many techniV ues-oricnlcd scientists will he attracted to wink on LB Hints because they provide interesting novel structures whose molecular architecture can be systematically controlled, The quality of (he floating monolayer ts also important and needs to he characterized as does the interlace between the first deposited monolayer and ihe substrate. Fluorescence microscopy and Brillouin and Fourier transform infrared spectroscopies are currently being used to address these problems. 

This volume covers a wide range of fundamental topics in coal maceral science that varies from the biological origin of macerals to their chemical reactivity. Several chapters report novel applications of instrumental techniques for maceral characterization. These new approaches include solid l3C NMR, electron spin resonance, IR spectroscopy, fluorescence microscopy, and mass spectrometry. A recently developed method for maceral separation is also presented many of the new instrumental approaches have been applied to macerals separated by this new method. The contributions in this volume present a sampling of the new directions being taken in the study of coal macerals to further our knowledge of coal petrology and coal chemistry. 

The use of fluorescence microscopy to characterize coal macerals is a successful recent innovation. 

Figure 1.49 shows an example of using confocal fluorescent microscopy to reveal microscopic features in a specimen. The specimen is low density polyethylene (LDPE) containing fluores-cently labeled silica particles. The particle size and distribution in the polymer matrix can be clearly revealed by 3D confocal microscopy. Thus, confocal microscopy provides us a new dimension in light microscopy for materials characterization, even though its applications in materials science are not as broad as in biology. 

This review describes some of the recent developments in materials which exhibit enhanced two-photon absorption that can initiate photopolymerization or up-converted emission. Various optical methods including femtosecond time-resolved pump-probe experiments to characterize the two-photon properties are discussed. Finally, the applications of two-photon processes to optical power limiting, up-converted lasing, 3-D data storage, 3-D micro-fabrication, two-photon fluorescence microscopy and bio-imaging, and two-photon photodynamic therapy are presented. 

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