Corrected absorbance

For example, The concentration is derived from a 6 point calibration curve by reading off the concentration, corresponding to the sample absorbance, corrected for the blank value, and multiplying it by the concentration factor . 

Fig. 3.5.7 Absorbance (corrected for ArH and the quartz substrate) of 10-layer (solid line), 20-layer (dashed line), 30-layer (triangles) and 40-layer (squares) HgAr films exposed to H2S for 15 min. Inset Absorbance of HgS in ArH (corrected for ArH and quartz) as a function of layer number at 350, 400, 500, 600, and 700 nm. (From Ref. 45.)...

Mobed, J. J., S. L. Hemmingsen, J. L. Autry, and L. B. McGowan. 1996. Fluorescence characterization of IHSS humic substances total luminescence spectra with absorbance correction. Environmental Science Technology 30 3061—3065. 

Infrared spectra were recorded on a Perkin-Elmer Model 983G double-beam spectrophotometer in the transmission mode using 3500 A thick PBTMSS films spin-coated and processed on polished NaCl plates. Spectral subtraction and absorbance correction to account for the decreased film thickness were used to isolate the silicon oxide absorption band at about... 

Fig. 2. Far ultraviolet spectra of a-amino acids. All amino acids were in aqueous solution (2 mm quartz cells) at pH 6, except cystine (pH 3). The dibasic acids were measured as hydrochlorides and the absorbance corrected by subracting the absorbance contribution of chloride ion. Measurements were made in a Beckman DK2a extented-UV range ratio-recording spectrophotometer with continuous nitrogen flushing below 2(KK) A (Sussman and Gratzer, 1962).

A photometric titration curve is a plot of absorbance (corrected for volume change) as a function of titrant volume. If conditions are chosen properly, the curve consists of two straight-line regions with different slopes, one occurring prior to the equivalence point of the titration and the other located well beyond the equivalence-point region the end point is taken as the intersection of extrapolated linear portions of the two lines. 

Jain, A., A. Chaurasia, and K. K. Verma. 1995. Determination of ascorbic acid in soft drinks, preserved fruit juices and pharmaceuticals by flow injection spectrophotometry Matrix absorbance correction by treatment with sodium hydroxide. Talanta 42 779-787. 

More surprisingly, complex 32 displayed ambient temperature luminescence in CH2CI2 solution ( em = 442 nm) with an impressive quantnm yield (O = 0.012, following self-absorbance correction) for an organometallic complex (Figure 15.29). Solutions of complex 32 in this and other solvents all... 

Polyacrylonitrile is one of the components of lacquer coatings used to moistureproof electrical equipment. The nitrile based at 4.48 n has been used by Adams and Swann [ ] for quantitative determination of the coating thickness as this is essentially free of interferences in this case. Samples are run in methyl isobutylketone or toluene and a horizontal baseline is drawn from the solution absorbance at 4.90 /I to 4.48 /x. Only one cell (200 n thick) is used and absorbance (corrected for baseline) is measured to give nitrile content. Standardization is from similar measurements on samples where the nitrile content is determined by Kjeldahl nitrogen analysis. 

Hardy and Young Eberhardt and Mostyn have all treated this effect Rogers has considered the influence of spectral slit width on the absorption of pharmaceuticals in the visible and ultraviolet regions. Lothian discussed the effects of finite slit width in differential absorptiometry and Back et aL recommended absorbance corrections for finite slit width with nonuniform absorbents. 

Mobed, J.J., Henuningsen, S.L., Autry, J.L., and McGown, L.B. (1996). Fluorescence characterization of IHSS humic substances Total luminescence spectra with absorbance correction. Environ. ScL Technol, 30, 3061-3065. 

---