Fission yeast cell cycle

Enoch, T and Nurse, P. (1990). Mutation of the fission yeast cell cycle control genes abolishes dependence of mitosis on DNA replication. Cell 60 665-673. 

Gautier, J., Norbury, C., Lohka, M., Nurse, P., and Mailer, J. (1988). Purified maturation-promoting factor contains the product of a Xenopus homologue of the fission yeast cell cycle control gene cdc2+. Cell 54 433-439. 

Hayles, J., Beach, D., Durkacz, B., and Nurse, P. (1986). The fission yeast cell cycle control gene cdc2 isolation of a sequence sucl that suppresses cdc2 mutant function. Mol Gen Genet 202, 291-3. 

P13 acts in the fission yeast cell division cycle. Addition of the proton to the kinase assay in vitro was able to rescue the defect in the Cdc2 mutant kinase activity P13 cont ns two tryptophans (Trp-71 and Trp-82) and seven tyrosine residues. The maximum of the fluorescence emission spectrum b localcd at 336 nm (>., 295 or 275 nm). Thus, tryptophan residues are responsiUe for the protdn fluorescence. Three lifetimes are found for the intensity dec, 0.6,2.9 and 6.1 ns, with fracUonal intensities of0.04,0.32 and 0.64, respectively. 

Booher, R., and Beach, D. (1986). Site-specific mutagenesis of cdc2+, a cell cycle control gene of the fission yeast Schizosaccaromyces pombe. Mol. Cell. Biol. 6 3523-3530. Booher, R. N., Alfa, C. E., Hyams, J. S., and Beach, D. H. (1989). The fission yeast cdc2/cdcl3/sucl protein kinase regulation of catalytic activity and nuclear localization. CeU 58 485-497. 

Bueno, A Richardson, H., Reed, S. I., and Russell, P. (1991). A fission yeast B-type cyclin functioning early in the cell cycle. Cell 66 149-159. 

Ducommun, B., Draetta, P., Young, P and Beach, D. (1990). Fission yeast cdc25 is a cell-cycle regulated protein. Bichem. Biophys. Res. Commun. 167 301-307. 

Jimenez, J., Alphey, L., Nurse, P, and Glover, D. M. (1990). Complementation of fission yeast cdc2,s and cdc25a mutants identifies two cell cycle genes from Drosophila a cdc2 homologue and string. EMBO J. 9 3565-3571. 

Krek, W., Marks, J., Schmitz, N Nigg, E. A., and Simanis, V. (1992). Vertebrate p34c 2 phosphorylation site mutants effects upon cell cycle progression in the fission yeast Schizosaccammyces pombe. J. Cell Sci. 102 43-53. 

Simanis, V., and Nurse, R (1986). The cell cycle control gene cdc2+ of fission yeast encodes a protein kinase potentially regulated by phosphorylation. Cell 45 261-268. 

Funabiki H, Hagan I, Uzawa S, Yanagida M 1993 Cell cycle-dependent specific positioning and clustering of centromeres and telomeres in fission yeast. J Cell Biol 121 961-976... 

Fission yeast S. pombe is also referred to as safe - it was used for beer production in South Africa. Along with S. cerevisiae it is a very well established tool for studying basic biological processes like control of cell cycle and DNA repair processes [88], and its genome sequence is known [89]. 

Ishiguro, J. (1989). An abnormal cell division cycle in an AIR carboxylase-deficient mutant of the fission yeast Schizosaccharomyces pomhe. Curr. Genet. 15,71-74. 

Hagan IM, Hyams JS. The use of cell division cycle mutants to investigate the control of micotubule distribution in the fission yeast Schizosaccharomycespombe. J Cell Sci 1988 89 343-357. 

B. Novak, Z. Pataki, A. Ciliberto, and J. J. Tyson, Mathematical model of the cell division cycle of fission yeast. Chaos 11, 277-286 (2001). 

For the fission yeast S. pombe, it has been possible to put together a very plausible model that leads to a halt in the cell cycle at the G2/M transition on damage of DNA and prevents entry into mitosis (Fig. 13.16). 

Okayama H., Nagata A., Jinno S. and Murakami H. (1996). Cell cycle control in fission yeast and mammals identification of new regulatory mechanisms. Advances in Cancer Research. 69, pp 17-62. 

Finally, 80 proteins localized to an abnormal intranuclear microtubule structure upon LMB treatment, while others simply occupied nucleus or nucleolus. Crml-dependent dynamic interchange of proteins between the nucleus and the microtubules may regulate microtubule organization during the cell cycle in fission yeast. Further analysis of individual proteins will reveal the involvement of Crml in the process for microtubule assembly and disassembly. 

A FIGURE 21-11 The fission yeast S. pombe. (a) Scanning electron micrograph of S. pombe cells at various stages of the cell cycle. Long cells are about to enter mitosis short cells have just passed through cytokinesis, (b) Main events In the... 

In 2001, the Nobel Prize in Physiology or Medicine was awarded to three cell cycle scientists. Sir Paul Nurse was recognized for his studies with the fission yeast 5. pombe, in particular for the discovery and characterization of the weel gene. What is the wee phenotype What did the characterization of the weel gene tell us about cell cycle control ... 

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