Yeasts budding

Hunter, T., Plowman, G.D. The protein kinases of budding yeast six score and more. Trends Biochent. Sci. 

Kron S.J. Gow N.A.R. (1995) Budding yeast morphogenesis signalling, cytoskeleton and cell cycle. 

Lew D.J. Reed S.l. (1995) Cell cycle cond ol of morphogenesis in budding yeast. Curr Opin Genet Dev, 5, 17-23. 

Epstein, C. B and Cross, F. R. (1992). CLB5 a novel B cyclin from budding yeast with a role in S phase. Genes Dev. 6 1695-1706. 

Fitch, I., Dahmann, C., Surana, U., Amon, A., Nasmyth, K., Goetsch, L., Byers, B., and Futcher, B. (1992). Characterization of four B-type cyclin genes of the budding yeast Saccharomyces cerevisiae. Mol. Biol. Cell 3 805-818. 

Nasmyth In the case of the weel system, not very much is known about how fission yeast is doing it. In budding yeast, it is the level of Cln3. 

The budding yeast securin has what appears to be a single stable partner, a 180 kDa protein called Espl (Ciosk et al 1998). In fission yeast, Cut2 had previously been found to be associated with Cut 1, an Espl homologue (Funabiki et al 1996b). 

Goshima G, Yanagida M 2000 Establishing biorientation occurs with precocious separation of the sister kinetochores, but not the arms, in the early spindle of budding yeast. Cell 100 619-633... 

Irniger S, Piatti S, Michaelis C, Nasmyth K 1995 Genes involved in sister chromatid separation are needed for B-type cyclin proteolysis in budding yeast. Cell 81 269-278 (erratum 1998 Cell 93 487)... 

Surana U, Amon A, Dowzer C, McGrew J, Byers B, Nasmyth K 1993 Destruction of the CDC28/CLB mitotic kinase is not required for the metaphase to anaphase transition in budding yeast. EMBO J 12 1969-1978... 

Gonc y Do you know whether the differences between mitosis and meiosis that you told us about in budding yeast also apply in mammals ... 

Nurse Another difference is that the mitotic cohesin persists in fission yeast meiosis, which is not the case in budding yeast. I think what is exciting about this, however, are the similarities, so we shouldn t get too worried by the differences. We have talked a lot about cohesin, but I wondered whether it might be worth having some discussion of the S phase suppression. 

Nurse In that context, in budding yeast there is nuclear migration and there are astral microtubules captured by the bud tip. The bud tip will have also been marking where cytokinesis will have been initiated. There might be some links there. 

Schaar Another connection between budding yeast and metazoan systems is that Ebl and its homologue in budding yeast, and also APC, are dynamically localized to the growing tips of microtubules. The connection isn t certain, but people place APC in the Wnt signalling pathway. Perhaps there is a non-nuclear pathway that is... 

Nasmyth In budding yeast in meiosis there is G2 arrest, which never occurs in mitotic cells. 

Phaeococcomyces exophialae forms slimy, mucoid, slow-growing, smooth colonies that are grayish black. Budding yeast cells which are at first subhyaline are abundant. With age, some of the cells become darker, with thickened cell walls. Some pseudohyphae usually are present. Hyphal development may become dominant in some isolates of this species with subsequent subculture. 

The budding yeast Saccharomyces cerevisiae is an extremely attractive eukaryotic model system for the study of genes involved in iron metabolism. This is because of its short generation time, the ease with which relatively large amounts of... 

Fig. 6.1. The F-box motif in human Skp2, budding yeast Cdc4, and human cyclin F (CycF). The conserved amino acids are highlighted.

The first class of DUBs discovered, the ubiquitin C-terminal Aydrolases (UCHs), is a relatively small class vith only four members in humans and one in budding yeast. UCHs are cysteine proteases related to the papain family of cysteine proteases. Most UCHs consist entirely of a catalytic core that has a molecular mass of about 25 kDa, although Bapl and UCH37 have C-terminal extensions [21, 22], All UCHs have a highly conserved catalytic triad consisting of the active-site cysteine, histidine, and aspartate residues that are absolutely required for function [23]. 

H. Budding yeast Dsk2p is a polyubiquitin-binding protein that can interact with the proteasome. Proc. Natl. Acad. Sci. (USA) 2002, 99, 745-750. 

Fig. 12.3. Subclasses of the CUE domain family. All human and budding yeast members of the CUE family have been aligned and subjected to neighbor-joining dendrogram analysis. In proteins having multiple domains, the domain number is indicated in square...

The CUE domain s propensity to bind ubiquitin was a quite recent discovery, and relatively little is known about its physiological role. Nevertheless, structural work done on this domain type has been instrumental for our understanding of ubiquitin recognition in general. Two independently solved structures of different CUE domains have been reported, both in isolation and in complex with ubiquitin [64, 65]. The NMR structure of the first CUE domain of the uncharacterized budding yeast protein Cue2 shows a three-helix bundle fold resembling that of the... 

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