Extraction page

Weaver, L. (1956). Separation of rare earths by liquid-liquid extraction, page 50 in Rare Earths in Biochemical and Medical Research A Conference Sponsored by the Medical Division, Oak Ridge Institute of Nuclear Studies, October 1955, Report No. ORINS-12, Kyker, G. C. and Anderson, E. B., Eds. (Office of Technical Services, Washington). 

Thyroid Extract, Pages 186-187, Greene Shepherd SummaryPlus Full Text + Links PDF (54 K)... 

The most abundant natural steroid is cholesterol. It can be obtained in large quantides from wool fat (15%) or from brain or spinal chord tissues of fat stock (2-4%) by extraction with chlorinated hydrocarbons. Its saturated side-chain can be removed by chromium trioxide oxidation, but the yield of such reactions could never be raised above 8% (see page 118f.). 

Extraction of rosin. Rosin resins are produced from three types of rosin, i.e. gum, tall oil, and wood. Extensive details about rosin resins extraction and derivatization can be found on page 269 in the book edited by Zinkel and Russell [18]. 

Extract air (ETA) classification Treated or untreated air that is removed from a space and discharged to outdoors. CEN/ TC 156 classifies extract air into four categories. (See top of page.)... 

Alphanumeric identifier of data source and page of source data was extracted from... 

The large pore structure of the TSK-GEL G6000PW allows it to separate large molecules such as pBR322 plasmid from contaminating RNAs and proteins in a much shorter time frame than other methods (23). A two column system of G6000PW (7.5 mm i.d. X 60 cm) was used to separate the cleared lysate and phenol extract of the plasmid as shown in Fig. 4.34 (page 130). The plasmid... 

To 6a-fluoro-16a-hydroxy-hydrocortisone 21-acetate, described by Mills et al, J. Am. Chem. Soc., volume 81, pages 1264 to 1265, March 5, 1959, there was added acetic anhydride in dry pyridine. The reaction mixture was left at room temperature overnight and was then poured with stirring into ice water. The resulting precipitate was filtered, washed with water and crystallized from acetone-hexane to give 6a-fluoro-16a-hydroxy-hydrocortisone-16a,21-diacetate. This was reacted with methane-sulfonyl chloride in dimethyl formamide in the presence of pyridine at 80°C for 1 hour. The mixture was cooled, diluted with water and extracted with ethyl acetate. The extract was washed with water, dried over anhydrous sodium sulfate and the ethyl acetate was evaporated. By recrystallization of the residue from acetone-hexane there was obtained 6a-fluoro-A <" -pregnadiene-16o ,17a,21-triol-3,20-dione 16a,21 diacetate. 

Sterile agar slants are prepared using the Streptomyces sporulation medium of Hickey and Tresner, J. Bact., vol. 64, pages 891-892 (1952). Four of these slants are inoculated with lyophilized spores of Streptomyces antibioticus NRRL 3238, incubated at 28°C for 7 days or until aerial spore growth is well-advanced, and then stored at 5°C. The spores from the four slants are suspended in 40 ml of 0.1% sterile sodium heptadecyl sulfate solution. A nutrient medium having the following composition is then prepared 2.0% glucose monohydrate 1.0% soybean meal, solvent extracted, 44% protein 0.5% animal peptone (Wilson s protopeptone 159) 0.2% ammonium chloride 0.5% sodium chloride 0.25% calcium carbonate and water to make 100%. 

The following pages will describe several examples of pyrochemical processing as applied to the recycle of plutonium, and will briefly review the fundamental chemistry of these processes. We shall review the conversion of plutonium oxide to plutonium metal by the direct oxide reduction process (DOR),the removal of americium from metallic plutonium by molten salt extraction (MSE), and the purification of metallic... 

Figure 10. Reversed-phase HPLC analysis of PAHs extracted from SRM 1649, urban dust/organics, with UV detection, preceded by normal-phase HPLC fractionation based on ring carbon number. (Reprinted from reference 72. Copyright 1984 American Chemical Society.) Continued on next page.

Figure 4.4 The general protocol for information extraction from an herbal text (A-E) is paired with case examples from our work with the Ambonese Herbal by Rumphius. For full caption see page 112.

Figure 4-4. Use of SDS-PAGE to observe successive purification of a recombinant protein. The gel was stained with Coomassie blue. Shown are protein standards (lane S) of the indicated mass, crude cell extract (E), high-speed supernatant liquid (H), and the DEAE-Sepharose fraction (D). The recombinant protein has a mass of about 45 kDa.

Figure 4-5. Two-dimensional lEF-SDS-PAGE.The gel was stained with Coomassie blue. A crude bacterial extract was first subjected to isoelectric focusing (lEF) in a pH 3-10 gradient. The lEF gel was then placed horizontally on the top of an SDS gel, and the proteins then further resolved by SDS-PAGE. Notice the greatly improved resolution of distinct polypeptides relative to ordinary SDS-PAGE gel (Figure 4-4).

The catalytic activities of the fortified wheat germ cell-free systems supplemented with each fraction were investigated (Fig. 2). As shown in Fig. 2, only 0 - 40 % ammonium sulfate fraction showed an enhancement in DHFR protein synthesis. This enhancement of protein experimental results and the fact that the various eukaryotic initiation factors are contained in synthesis was also confirmed by SDS-PAGE and autoradiography (Fig. 3). From the above 0-40 % ammonium sulfate fraction [5, 6], it can be concluded that the amount of initiation factors in a conventionally prepared wheat germ cell-fi extract is deficient for the translation of DHFR with internal ribosome entry site. Therefore, it needs to supplement a wheat germ cell-free extract with the fraction containing the limited initiation factors for the efficient protein translation, and this fortified cell-free system can be easily made by simple... 

Figure 4. Purification of PemB from E. coli K38 pGPl-2/pPME6-5 cells. Proteins were separated by urea-SDS-PAGE. Lane 1, induced cell lysate lane 2, soluble protein fraction from induced cells lane 3, membrane fraction from non-induced cells lane 4, membrane fraction from induced cells lane 5, membrane proteins not extracted by Triton X-100 lane 6, membrane proteins extracted by Triton X-100 lane 7, PemB purified by preparative electrophoresis. The molecular weight standard positions are indicated.

The specifications and standardization include raw materials, preparation method of the standard solution, concentration of proteins, and the main band on SDS-PAGE. The outline of the procedure for preparation of the calibrators is shovm in Eig. 4.2. Table 4.5 shows the raw materials and the preparation method of the initial extract. To prepare the calibrators, the raw materials are extracted by the standard solution containing SDS and mercaptoethanol. The initial extract is prepared by centrifugation and filtration of the extract. The diluted extract is then prepared by 10-fold dilution of the initial extract with phosphate-buffered saline (PBS pH 7.4). The protein concentration of the diluted extract is assayed using the 2-D Quant kit (Amersham Bio Sciences). The standard solution is then... 

Zakay-Rones, Zichria, Ph.D., Varsano, Noemi, M.Sc., Zlotnik, Moshe, M.D., Manor, Orly, Ph D., Regev, Liora, Schlesinger, Miriam and Mumcuoglu, Madeleine, Ph.D., "Inhibition of Several Strains of Influenza Virus in Vitro and Reduction of Symptoms by an Elderberry Extract, The Journal of Alternative and Complementary Medicine, Volume 1, Number 4, 1995, pages 361369. 

This document, which Newton likely wrote in the mid-1670s, is part of an eight-page manuscript now housed at Yale University. The manuscript contains extracts from Newton s favorite alchemist, the American writer George Starkey (1628-1665). Starkey s Marrow of Alchemy (1654-5), the work Newton cites here, was published under Starkey s pseudonym, Eirenaeus Philalethes ("a peaceful lover of truth")"... 

An extract from Bernard of Trevisan, Le Texte dAlchymie et le Songe-Verd, Paris, 1695. (pages 87-92.)"... 

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