Next page Extraction

Figure 10. Reversed-phase HPLC analysis of PAHs extracted from SRM 1649, urban dust/organics, with UV detection, preceded by normal-phase HPLC fractionation based on ring carbon number. (Reprinted from reference 72. Copyright 1984 American Chemical Society.) Continued on next page.

Crude sample was extracted with anhydrous ethyl ether Next Page... 

Figure 4, Extractable organic profile (ethyl ether/hexane, 5/95) of a random lot of flexible PUF reconstructed ion chromatograms (GC-MS). A, solvent extract B, Soxhlet blank. Component identification (scan number, component) 232, phenol 391, hexanoic acid, 2-ethyl 490, 2,4- or 2,6-toluene diisocyanate (TDI) 507,2-propanamine, 2-methyl 592, phenol, 2,6-bis-(l,l-dimethylethyl)-4-methyl 696, chloroctane (isomer) 737, anthracene-dw (internal standard) 1047, isooctane, ethenyloxy. Continued on next page.

Solution The relevant output from the AIM facility is given on the next page (note that the three portions we have extracted are from separate sections of the AIM output). We are interested in attractors—atoms—numbers 1, 3 and 4, which are the carbon atoms. The AIM method places the positive charge near the hydrogen atoms, while the carbon atoms have very little excess positive charge (although there is slightly more on the central carbon). 

The removal of the O atom from the 0 ion leaves behind two electrons that need a home. We can imagine them flooding onto the Fe ions that still cluster round the 0" ions, even in the fluid (Figure 4.4 on the next page). It is easy to do the arithmetic for every three O atoms extracted by CO molecules... 

To get the product, the acetonitrile is evaporated off under a vacuum. The residue is then dissolved in a mixture of 450 ml of chloroform and 60 ml ice water. The chloroform layer is then separated, and the water layer is then extracted four times with 150 ml portions of chloroform. The chloroform extracts are next combined with the original chloroform layer. This combined chloroform solution is next washed a couple of times with 100 ml portions of cold water to remove amine salts. The combined chloroform layers are then dried with a little sodium sulfate, and the chloroform evaporated away under a vacuum to give a solid residue weighing about 10 grams which is a mixture of LSD and iso-LSD and other by products. The other byproducts are the result of non-specific acylation as reported by Garbrecht in the Journal of Organic Chemistry Volume 24, pages 368-... 

In the first experiment, treatment of murine thymus extracts with fluorescent peptide epoxyketone MVB-003 followed by SDS-PAGE revealed, next to the expected bands denoting the catalytic residues of constitutive proteasomes and... 

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