Expression epithelial cell differentiation

Hino K, Tsuchiya K, Fukao T, Riga K, Okamoto R, Kanai T, Watanabe M (2008) Inducible expression of microRNA-194 is regulated by HNF-1 alpha during intestinal epithelial cell differentiation. RNA 14(7) 1433-1442... 

Momma, Y, Nagineni, CN, Chin, MS, Srinivasan, K, Detrick, B, and Hooks, JJ, 2003. Differential expression of chemokines by human retinal pigment epithelial cells infected with cytomegalovirus. Invest Ophthalmol... 

Eckmann, C., Jung, EEC., Schurer-Maly, C., Panja, A., Wroblewska, E.M. and Kagnoff, M. (1993) Differential cytokine expression by human intestinal epithelial cell lines regulated expression of IL-8. Gastroenterology 105, 1689-1697. 

E., Brattain, M. G., Zweibaum, A., Epithelial polarity, villin expression, and enterocytic differentiation of cultured human colon carcinoma cells a survey of twenty cell lines, Cancer Res. 1988, 48, 1936-1942. 

Ichikawa JK et al. Interaction of Pseudomonas aeruginosa with epithelial cells identification of differentially regulated genes by expression microarray analysis of humans. Proc Natl Acad Sci USA 2000 97 9659-9664. 

Cultured nasal cells are reliable models for drug transport and metabolism studies, since they are known to express important biological features (e.g. tight junctions, mucin secretion, cilia, and various transporters), resembling those found in vivo systems. Moreover, easy control of experimental conditions as well as separation of the permeation step from the subsequent absorption cascade is also possible. A relatively simple primary culture condition using human nasal epithelial cells for in vitro drug transport studies has been established and applied in transport and metabolism studies of drugs. It is known that the culture condition and/or selection of culture media are critical in the recapitulation of well-differentiation features of in vivo nasal mucosal epithelium [46],... 

Christensen PJ, Kim S, Simon RH, Toews GB, Paine R 3rd (1993) Differentiation-related expression of ICAM-1 by rat alveolar epithelial cells. Am J Respir Cell Mol Biol 8( 1 ) 9—15... 

Fuchs S, Hollins AJ, Laue M, Schaefer UF, Roemer K, Gumbleton M, Lehr CM (2003) Differentiation of human alveolar epithelial cells in primary culture— Morphological characterisation and expression of caveolin-1 and surfactant protein-C. Cell Tissue Res 311 31-45... 

In another approach, Parnigotto and coworkers reconstructed corneal structures in vitro by using corneal stroma containing keratocytes to which corneal epithelial cells from bovine primary cultures were overlaid [73], However, this particular corneal model did not contain an endothelial layer. This model was histochemically characterized and the toxicity of different surfactants was tested using MTT methods. This stroma-epithelium model has been reported to show a cornea-like morphology, where a multilayered epithelial barrier composed of basal cells (of a cuboidal shape) and superficial cells (of a flattened shape) is noted. Furthermore, the formation of a basement membrane equivalent and expression of the 64-kDa keratin were reported, indicating the presence of differentiated epithelial cells. The toxicity data for various surfactants obtained with this model correlate well with those seen by the Draize test [73], However, this corneal equivalent was not further validated or used as a model for permeation studies. 

Gaudreault M, Carrier P, Larouche K, Leclerc S, Giasson M, Germain L, Guerin SL. Influence of spl/sp3 expression on corneal epithelial cells proliferation and differentiation properties in reconstructed tissues. Invest Ophthalmol Vis Sci 44 1447-1457 (2003). 

Sizemore N, Kasturi L, Gorodeski G, Eckert RL, Jetten AM, and Rorke EA [1993] Retinoid regulation of human ectocervical epithelial cell transglutaminase activity and keratin gene expression. Differentiation 54 219-225... 

Choo CK, Rorke EA, and Eckert RL [1993] Calcium regulates the differentiation of human papillomavirus type 16 (HPV16) immortalized ectocervical epithelial cells, but not the expression of the papillomavirus E6 and E7 oncogenes. Exp Cell Res 208 161-169... 

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