Escherichia coli antibody

Smiley, J. A., andBenkovic, S.J. (1994). Selection of catalytic antibodies for a biosynthetic reaction from a combinatorial cDNA library by complementation of an auxotrophic Escherichia coli antibodies for orotate decarboxylation. Proc, Natl. Acad. Sci. USA, 91(18), 8319-8323. 

A number of allergens from both honey bee and vespid venoms have been cloned and expressed by either Escherichia coli or baculovirus-infected insect cells (table 1) phospholipase Aj [20], hyaluronidase [21], acid phosphatase [13] and Api m6 [14] from honey bee venom, as well as antigen 5 [22], phospholipase A and hyaluronidase [23] from vespid venom, and dipeptidylpeptidases from both bee and Vespula venoms [15, 16]. Their reactivity with human-specific IgE antibodies to the respective allergens has been documented [11-16, 22, 23] and their specificity is superior... 

Starnes, H. F Pearee, M. K., Tewari, A., Yim, H. H., Zou, J. C., and Ambrams, J. S., Anti-IL-6 monoclonal antibodies protect against lethal Escherichia coli infection and lethal tumor necrosis factor-a challenge in mice. J. Immunol. 145,4185-4191 (1990). 

Kusunoki, H. Latiful Bari, M. Kita,T. Sugii, S. Uemura,T. Flow cytometry for the detection of enterohaemorrhagic Escherichia coli 0157 H7 with latex beads sensitized with specific antibody. J. Vet. Med. 2000,47,551-559. 

Laden JC, Philibert P, Torreilles F, Pugniere M, Martineau P (2002) Expression and folding of an antibody fragment selected in vivo for high expression levels in Escherichia coli cytoplasm. Res Microbiol 153 469-474... 

Hamaguchi, Y., Yoshitake, S., Ishikawa, E., Endo, Y., and Ohtaki, S. (1979) Improved procedure for the conjugation of rabbit IgG and Fab antibodies with b-D-galactosidase from Escherichia coli using N,N -o-phenylenedimaleimide. J. Biochem. (Tokyo) 85, 1289-1300. 

Sastry, L., Alting-Mees, M., Huse, W.D., Short, J.M., Sorge, J.A., Hay, B.N., Janda, K.D., Benkovic, S.J., and Lerner, R.A. (1989) Cloning of the immunological repertoire in Escherichia coli for generation of monoclonal catalytic antibodies Construction of a heavy chain variable region-specific cDNA library. Proc. Natl. Acad. Sci. USA 86, 5728-5732. 

I.H. Boyaci, Z.P. Aguilar, M. Hossain, H.B. Halsall, CJ. Seliskar, and W.R. Heineman, Amperometric determination of live Escherichia coli using antibody-coated paramagnetic beads. Anal. Bioanal. Chem. 382, 1234-1241 (2005). 

Kelley, R.F., M.P O Connell, P. Carter, L. Presta, C. Eigenbrot, M. Covarruabias, B. Snedecor, R. Speckart, G. Blank, D. Vetterlein, and C. Kotts. 1993. Characterization of humanized anti-pl85HER2 antibody fab fragments produced in Escherichia coli. ACS Symp Ser 526 218-239. 

Thankavel, K., Madison, B., Ikeda, T., Malaviya, R., Shah, A. H., Arumugen, P. M., and Abraham, S. N. (1997). Localization of a domain in the FimH adhesin of Escherichia coli type 1 fimbriae capable of receptor recognition and use of a domain-specific antibody to confer protection against experimental urinary tract infection. /. Clin. Invest. 100, 1123-1126. 

Bacterial pathogens are relatively large targets (> 1pm) and therefore, their presence can be detected directly with an optional amplification by secondary antibodies (sandwich assay). Examples of foodbome bacterial pathogens detected by SPR biosensors include Escherichia coli (detection limit 5x10 cfii/ml " " ), Listeria monocytogenes (detection limit 1 O cfii/ml " ) and Salmonella enteritidis (detection limit lO cfii/ml" ). 

Hahm, B. K., and Bhunia, A. K. (2006). Effect of environmental stresses on antibody-based detection of Escherichia coli 0157 H7, Salmonella enterica serotype Enteritidis and Listeria monocytogenes. J. Appl. Microbiol. 100,1017-1027. 

Manjarrez-Hernandez, H. A., Gavilanes-Parra, S., Chavez-Berrocal, E., Navarro-Ocana, A., and Cravioto, A. (2000). Antigen detection in enteropathogenic Escherichia coli using secretory immunoglobulin A antibodies isolated from human breast milk. Infect. Immun. 68, 5030-5036. 

In 1990, the first plant-made vaccines were performed via expression of Streptococcus mutans surface protein A in transgenic tobacco, followed by oral immunization of mice with the same plant material (Fischer and Emans, 2000). This transgenic plant material was later shown to successfully induce an antibody response through a demonstration that serum from immunized mice could react with intact S. mutans. Plants were also developed that expressed Escherichia coli enterotoxin B subunit (LT-B) and that exhibited successful inducement of both mucosal and serum antibody responses (Tacket, 2005). These initial experiments led to a cornucopia of studies involving generations of plant-made vaccines and therapeutic proteins and their applications in medicine. 

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