Heavy chains variable regions

Sastry, L., Alting-Mees, M., Huse, W.D., Short, J.M., Sorge, J.A., Hay, B.N., Janda, K.D., Benkovic, S.J., and Lerner, R.A. (1989) Cloning of the immunological repertoire in Escherichia coli for generation of monoclonal catalytic antibodies Construction of a heavy chain variable region-specific cDNA library. Proc. Natl. Acad. Sci. USA 86, 5728-5732. 

Field, H., G.T. Yarranton, and A.R. Rees, Expression of mouse immunoglobulin light and heavy chain variable regions in Escherichia coli and reconstitution of antigen-binding activity. Protein Eng, 1990. 3(7) 641-7. 

OKT3 heavy chain variable region [Mus musculus] GI 1565186 OKT3 light chain [Mus musculus] GI 641464... 

Immunoglobulin heavy chain variable region [Mus musculus] ... 

Pascual, V., Capra, J.D. (1991). Human immunoglobulin heavy-chain variable region genes, organization, polymorphism, and expression. Adv. Immunol., 49, 1-74. 

Schroeder, H.W., Walter, M.A., Hofker, M.F., et al. (1988). Physical linkage of a human immunoglobulin heavy chain variable region gene segment to diversity and joining region elements. Proc. Natl. Acad. Sci. USA, 85, 8196-8200. 

Bothwell, A.L.M., Paskind, M., Reth, M., Imanishi-Kari, T., Rajewsky, K., Baltimore, D. (1981). Heavy chain variable region contribution to the NPb family of antibodies somatic mutation evident in a y2a variable region. Cell 24,625-637. 

Brodeur, P.H. Riblet, R. (1984). The immunoglobulin heavy chain variable region (Igh-V) locus in the mouse. I. One hundred Igh-V genes comprise seven families of homologous genes. Eur. J. Immunol. 14,922-930. 

Early, P., Huang, H., Davis, M., Calame, K., Hood, L. (1980a). An immunoglobulin heavy chain variable region gene is generated from three segments of DNA VH, D and JH. Cell 19,981-992. 

Giusti, A.M., Chien, N.C., Zack, D.J., Shin, S.-U., Scharff, M.D. (1987). Somatic diversification of SI07 from an antiphosphorylcholine to an anti-DNA autoantibody is due to a single base change in its heavy chain variable region. Proc. Natl. Acad. Sci. USA 84, 2926-2930. 

Livant, D., Blatt, C., Hood, L. (1986). One heavy chain variable region gene segment subfamily in the BALB/c mouse contains 500-1000 or more members. Cell 47,461-470. 

Siekevitz, M., Huang, S.Y., Gefter, M.L. (1983). The genetic basis of antibody production a single heavy chain variable region gene encodes all molecules bearing the dominant anti-arsonate idiotype in the strain A mouse. Eur. J. Immunol. 13, 123-132. 

Willems van Dijk, K., Milner, L.A., Sasso, E.H., Milner, E.C.B. (1992). Chromosomal organization of the heavy chain variable region gene segments comprising the human fetal antibody repertoire. Proc. Natl. Acad. Sci. USA 89, 10430-10434. 

D5. Demaison, C., Chastagner, P., Theze, J., and Zouali, M., Somatic diversification in the heavy chain variable region genes expressed by human autoantibodies bearing a lupus-associated nephritogenic anti-DNA idiotype. Proc. Natl. Acad. Sci. USA 91, 514-518 (1994). 

Construction of genes encoding the light and heavy chain variable regions... 

Crouzier, R., T. Martin, and J. L. Pasquali. 1995. Heavy chain variable region, light chain variable region, and heavy chain CDR3 influences on the mono- and polyreactivity and on the affinity of human monoclonal rheumatoid factors. J. Immunol. 154 4526-4535. 

Fig. 2. The light chain variable region is encoded by two separate gene segments, V and J. The heavy chain variable region is encoded by three gene segments, V, D and J.

The light and heavy chain variable region sequences are isolated from human blood cells by using polymerase chain reaction (PCR see Chapter 2), and are cloned into suitable phage or phage-plasmid... 

Previous studies by Coker et al. [41] showed evidence that a local clonal expansion, somatic hypermutation and class switching occurs in the nasal mucosa. More specifically, they found a biased use in the repertoire of IgE heavy-chain variable regions (VH) [44], The significant bias in tissue towards Vh5 suggests that a local event, possibly superantigens, may cause the VH5 bias. Such a Vh5 bias was also found in other diseases, especially in atopic dermatitis, a disease mediated by S. aureus and its enterotoxins. 

Antigenic Features of Immunoglobulins B. Heavy-Chain Variable Region Determinants... 

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