Escherichia coli, expression and

Miki Y, Morales M, Ruiz-Duenas FJ et al (2009) Escherichia coli expression and in vitro activation of a unique ligninolytic peroxidase that has a catalytic tyrosine residue. Protein Express Purif 68 208-214... 

Yan L, He-Chun Y, Hong W, GuO-Feng L (2003) Molecular cloning, Escherichia coli expression and genomic organization of squalene synthase gene fiom Artemisia annua L. Acta Botanica Sinica 45(5) 608-613... 

Shimomura, O., and Inouye, S. (1999). The in situ regeneration and extraction of recombinant aequorin from Escherichia coli cells and the purification of extracted aequorin. Protein Expression and Purification 16 91-95. 

Rodriguez, S., Schroeder, K.T., Kayser, M.M. and Stewart, J.D. (2000) Asymmetric synthesis of /3-hydroxy esters and alpha-alkyl-beta-hydroxy esters by recombinant Escherichia coli expressing enzymes from baker s yeast. The Journal of Organic Chemistry, 65 (8), 2586-2587. 

The regioselectivity of a Rhodococcus rhodochrous nitrilase has been demonstrated for the conversion of 5-fluoro-l,3-dicyanobenzene to 5-fluoro-3-cyano-benzoic acid [62]. The nitrilase was expressed in an Escherichia coli transformant, and a cell-free extract was employed as catalyst (0.14wt% cell-free extract) in 0.1m sodium phosphate buffer (pH 7.2) at 25 °C containing 0.18 m 5-fluoro-l,3-dicyanobenzene. After 72 h, the conversion was >98% and the reaction was stopped by addition of phosphoric acid (pH 2.4) to yield 5-fluoro-3-cyano-benzoic acid as a crystalline product (97% isolated yield). 

Parikh, A., Gillam, E.M. and Guengerich, F.P. (1997) Drug metabolism by Escherichia coli expressing human cytochromes P450. Nature Biotechnology, 15, 784—788. 

Yazdani, S. and Mukherjee, K. (2004) Continuous-culture studies on the stability and expression of recombinant streptokinase in Escherichia coli. Bioprocess and Biosystems Engineering, 24, 341-346. 

R. Frothingham, W. A. Meeker-0 Connell, E. A. S. Talbot, J. W. George, K. N. Kreu-zer, Identification, Cloning, and Expression of the Escherichia coli Pyrazinamidase and Nicotinamidase Gene, pncA , Antimicrob. Agent Chemother. 1996, 40, 1426-1431. 

Armed with this new tool, Schena et al. (1996) created a microarray of 1,046 human cDNAs of unknown sequence. They were derived from human peripheral blood lymphocyfes fransformed wifh Epsfein-Barr virus. Suitably sized inserts [>600 base pairs (bp)] were cloned into a lambda vector, subsequently infected into an Escherichia coli strain, and finally amplified by polymerase chain reaction (PCR) using 5 -amino-modified primers. The resulting 5 -amino-modified cDNA amplicons were then arrayed onto sily-lated microscope slides. Next, the expression levels in human Jurkat cells undergoing heat shock or phorbol ester induction were examined. 

Anakinra is a nonglycosylated form of the human IL-1 receptor antagonist (IL-lra). It is produced in a recombinant Escherichia coli expression system and has an additional methionine residue at its amino terminus. In rheumatoid arthritis patients, the amount of naturally occurring IL-lra in the synovial fluid is not sufhcient to counteract the high levels of locally produced IL-1. Anakinra acts as a competitive antagonist of the type 1 IL-1 receptor and decreases the pain and inflammation produced by IL-1. It is administered as a daily subcutaneous injection. 

Klyushnichenko, V., Tishkov, V. andKula, M.R. (1997) Rapid SDS-Gel capillary electrophoresis for the analysis of recombinant NADP(+)-dependent formate dehydrogenase during expression in Escherichia coli cells and its purification. J... 

Lundstrom K, Tilgmann C, Peranen J, Kalkkinen N, Ulmanen I. Expression of enzymatically active rat liver and human placental catechol- O-m ethyltransferase in Escherichia coli purification and partial characterizaton of the enzyme. Biochimica et Biophysica Acta 1992 1129 149-154. 

Zhang, W.W. Jiang, W.H. Zhao, G.P. Yang, Y.L. Chiao, J.S. Expression in Escherichia coli, purification and kinetic analysis of the aspartokinase and aspartate semialdehyde dehydrogenase from the rifamycin SV-producing Amycolatopsis mediterranei U32. Appl. Microbiol. Biotechnol., 54, 52-58 (2000)... 

Khan, A. A. Walia, S. K. (1990). Identification and localization of 3-phenylcatechol dioxygenase and 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase genes of Pseudomonas putida and expression in Escherichia coli. Applied and Environmental Microbiology, 56, 956-62. 

Nakamura, K., Iwasaki, Y. Hattori, T. (1980). An improved Escherichia coli expression vector for the construction and identification of full-length cDNA clones. Gene 44, 347-51. 

Simmons, L. C., Reilly, D., Klimowski, L., Raju, T. S., Meng, G., Sims, P., et al. (2002) Expression of full-length immunoglobulins in Escherichia coli rapid and efficient production of aglycosylated antibodies. J. Immunol. Methods 263, 133-147. 

Strong, L.C., H. McTavish, M.J. Sadowsky, and L.P. Wackett (2000). Field-scale remediation of atrazine-contaminated soil using recombinant Escherichia coli expressing atrazine chlorohydrolase. Environ. Microbiol., 2 91-98. 

M Anderlund, TL Nissen, J Nielsen, J Villadsen, J Rydstrom, B Hahn-Hagerdal, MC Kielland-Brandt. Expression of the Escherichia coli pntA and pntB genes, encoding nicotinamide nucleotide transhydrogenase, in Saccharomyces cerevisiae and its effect on product formation during anaerobic glucose fermentation. Appl Environ Microbiol 65 2333-2340, 1999. 

DC Kaslow, S Hill. Cloning metabolic pathway genes by complementation in Escherichia coli. Isolation and expression of Plasmodium falciparum glucose phosphate isomerase. J Biol Chem 265 12337-12341, 1990. 

J Crosby, DH Sherman, MJ Bibb, WP Revill, DA Hopwood, TJ Simpson. Polyketide synthase acyl carrier proteins from Streptomyces. Expression in Escherichia coli, purification and partial characterization. Biochim Biophys Acta 1251 32-42, 1995. 

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