Haematoxylin and eosin

H2O2 The chemical symbol for hydrogen peroxide Hag Haemagglutinin Hag-1, Hag-2 Cleaved haemagglutinin subunits-1, -2 H E Haematoxylin and eosin hIL Human interleukin Hb Haemoglobin... 

Fig. 18.2. Histological appearance of haematoxylin and eosin-stained jejunum of (A) uninfected control, with villi and crypts of normal lengths (B) day 13 p.i.

For morphological analyses, internal organs of mice were fixed in 10.0% neutral formalin they were processed by the standard histological technique and paraffin sections were stained in haematoxylin and eosin. Changes in internal organs of mice were assessed by a semi-quantitative method with divisions into four gradations (points) — not detected (0), l-t, 2+, 3-I-. 

Fig. 25.2 Survival of a split-thickness skin graft applied over Permacol paste. SSG day 0, assessed 6 days post grafting, haematoxylin and eosin, xlOO. Epithelium of a split-thickness skin graft (SSG) was viable and well-structured. Dermal component of the graft was intimately attached to the well-cellularised Permacol paste PP) with minimal signs of the inflammatory reaction. Dermal substitute biomaterial integrated into the full-thickness wound via granulation tissue in growth present at the paste and wound bed (WB) interface...

Fig. 19 Histological preparation (haematoxylin and eosin staining) of square sections, a BASYC in the middle region of the interposition four weeks after implantation in the carotid artery of the rat, b untreated carotid artery of the rat. - endothelial cell layer. BASYC layer...

Histological stains Wright-Giesma, haematoxylin, and eosin. 

Cell fixative - a haematoxylin and eosin stain (e.g. Rapi-Diff II, Diachem Diagnostic Developments, Southport, UK)... 

Fresh haematoxylin and eosin (H E) slides from blocks listed in (3). 

Specimens of M reevesi were obtained from the collection of Norma Chapman, which is derived from feral populations in the U.K. Thirty adult males and females were examined. Preorbital sacs and Harderian glands were dissected out, fixed in 10% formalin, processed into paraffin and sectioned at 7 - 10 pm. Alternate slides of both the preorbital sac and the Harderian gland were stained with either haematoxylin and eosin or Mallory s stain. The remaining Harderian gland slides were treated with histochemical stains mucosubstances were detected by the periodic acid-Schiffs (PAS) method, whereas bromphenol blue (BPB) was used to detect protein (Barka and Anderson, 1965). For lipid detection, frozen Harderian gland sections (15Dm) were stained with the supersaturated isopropanol method (Oil Red O Lillie, 1961), using exposure to 70% alcohol as a control. 

Sternal glands for histological study were excised from four male and four female freshly deceased adults and placed into 10% buffered formalin for 24 h. Glands were dehydrated in ethanol, cleared in xylene, cut into 3-mm sections, and embedded in paraplast. Serial lO-p cross-sections were stained with haematoxylin and eosin. 

Sporozoite of a piasmodium (a), ring-forms and gametocytes of Plasmodium falciparum in human blood (b), and a micrograph of a placenta from a stillbirth due to maternal malaria. (Haematoxylin and eosin stain) (c). Red blood cells are anuclear blue/black staining In bright red structures (red blood cells) Indicate foreign nuclei from the parasites. 

Both ovaries were fixed in Bouin s fluid, serially sectioned (7 ym thick) and stained with haematoxylin and eosine. The large ovarian follicles (types 6, 7, 8, atretic) were classified according to Pedersen and Peters (1968). 

Fig. 2. A photomicrograph in the caudal portion of a nine-week old fetal mandible. Notice the wide separation of Meckel s cartilage (me) from the mesenchymal tissue in the midline (m). t = tongue It = labial bony trabeculae. (Haematoxylin and Eosin x 20).

Fig. 12. A photomicrograph of a two-month old infant mandible. Notice that the caudal portion of the mental ossicle (mo) had started to fuse with both hemimandibles (arrow), while its rostral portion was still separated from the hemimandibles by a rim of mesenchymal tissue continuous with the midline mesenchyme (m). (Haematoxylin and Eosin X 20).

Fig. 14. A photomicrograph of a five-month old irrfant mandible, showing the complete fusion of the mental ossicle (mo) with the hemimandibles (hem) forming a single bony trabecula (b). Rostrally, remains of the symphyseal tissue (m) still separate the hemimandibles. The arrows show the direction of fusion between the mental ossicle and each hemimandible. (Haematoxylin and Eosin x 100).

Fig. 16. A photomicrograph of a one-year old infant mandible, showing a complete closure of the symphysis menti resulting in a single mandible. Notice the complete midline encroachment (line) by bony trabeculae (b). (Haematoxylin and Eosin x 20).

Fig. 199. Epithelioid-cell granulomatosis interpreted as Morbus Boeck. Mediastinal lymph node of a male patient born in 1948 (Nordwestkrankennaus Frankfort on the Main, autopsy 2109/79). - The slide shows Langhans giant cells but also some foreign body type giant cells. Fixed by immersion into formalin solution. Paraffin embedding. Haematoxylin and eosin. Objective Reichert PI 25x/0.45/oo/o.l7. Green filter. (Material provided by Prof. H5er+)...

Fig. 205. Cotton pellet granuloma 7 days after subcutaneous implantation in a 120 g male Sprague-Dawley rat (No. 3 February 4, 1969). Fixed by immersion in Bonin s fluid. Paraplast 4 pm. Haematoxylin and eosin. Objective Leitz PI 40/0.65. Leitz-Orthomat. Agfachrome 50 L professional...

In the Mongolian gerbil bilateral carotid occlusion model there was a substantial cell loss of 40-50%, measured in haematoxylin and eosin-stained section, 5 days post-insult (Stamford et al. 1999). Ascorbic add (500 mg per kg x day intraperi-toneally for S /z days, first dose 1 h before occlusion) caused significant striatal protection (cell loss reduced from 49 % to 20 %). After occlusion of the right carotid artery for 20 min, Hartmann et al. (1973) found enlarged mitochondria in the cytoplasm of the neurones of ipsilateral caudate nucleus. 

Fig. 350. An extensive and two smaller cerebral metastases of an undifferentiated solid carcinoma with numerous atypical mitoses. Primary tumour unknown. Brain Research Institute at the Department of Neurology, University of Leipzig (49/ 43). Haematoxylin and eosin...

Figure 2 See color insert.) Renal biopsy from a patient with MPA demonstrating crescentic glomerulonephritis with focal and segmental change and an area of fibrinoid necrosis, shown hy arrow. (Haematoxylin and Eosin x200).

All rats in study 1 dosed with 0,10, or 40mg/kg/day NTBC were killed at week 14. Rats dosed at 2mg/kg/day NTBC were terminated at week 21. The eyes of rats dosed at 2mg/kg/day NTBC were removed, fixed in Davidson s solution, routinely processed, paraffin wax embedded, cut at 5 im and then stained with haematoxylin and eosin. All sections were examined by light microscopy. 

The preparation of a tissue specimen of appropriate thickness from such as a biopsy sample (see also Chapter 5) usually follows standard histology practices, particularly since it is a common practice to microtome two parallel adjacent sections of near equivalent thickness. One of these sections is stained in a conventional way, most commonly with haematoxylin and eosin (H E), for... 

Optical material After sectioning, the tissue slices are mounted on IR transparent supportive substrates such as Cap2 windows for transmission-type measurements. Cap2 is a popular window material in IR spectroscopy because it is almost insoluble in water and thus allows post-staining of the sections by haematoxylin and eosin (H E). Furthermore, compared with other window... 

Cryo-microtomed tissue sections, nominal thickness 5 pm, were mounted on 0.5 mm thickness BaF2 windows and their spectra were recorded in transmission mode. Parallel sections were stained conventionally (with haematoxylin and eosin H E) to facilitate the identification of regions of particular interest. 

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