Endothelial cell colonization

Stoppato, M., Stevens, H.Y., Carletti, E., Migharesi, C., Motta, A., Guldberg, R.E., 2013. Effects of silk fibroin fiber incorporation on mechanical properties, endothelial cell colonization and vascularization of PDLLA scaffolds. Biomaterials 34,4573-4581. 

Santos, M.I., Tuzlakoglu, K., Fuchs, S., Gomes, M.E., Peters, K., Unger, R.E., Piskin, E., Reis, R.L., Kirkpatrick, C.J., 2008. Endothelial cell colonization and angiogenic potential of combined nano- and micro-fibrous scaffolds for bone tissue engineering. Biomateiials 29, 4306-4313. 

Santos, M. 1., etai.. Endothelial Cell Colonization and Angiogenic Potential of Combined Nano-and Micro-fibrous Scaffolds for Bone Tissue engineering. Biomaterials, 2008,29(32), 4306-4313. 

Returning to bacteria, PC also appears to play a role in infection of humans by pathogenic strains/species by allowing colonization and invasiveness due to interaction with appropriate receptors on host endothelial cells (reviewed by Harnett and Harnett, 1999). This may act as a double-edged sword, however, as the PC on the surface of the bacteria can be targeted by both the innate and adaptive immune responses and indeed such responses appear to play a role in the control of H. influenzae and S. pneumoniae, respectively, in humans (reviewed by Harnett and Harnett, 1999). 

Breast Cancer Resistance Protein (BCRP, also known as MXR or ABCP), first cloned from mitoxantrone and anthracycline-resistant breast and colon cancer cells [188, 189] is a half-transporter efflux pump believed to function as a homo-or hetero-dimer. Following its identification, BCRP-mediated drug resistance was observed for topoisomerase inhibitors including camptothecins [190, 191] and in-dolocarbazoles [192]. In normal tissues, BCRP was detected in placental syncytio-trophoblasts, hepatocyte canalicular membrane, apical intestinal epithelia and vascular endothelial cells [193]. These findings support the important role BCRP plays in modulating topotecan bioavailability, fetal exposure and hepatic elimination [194]. Considering that the substrates and tissue distributions for BCRP overlap somewhat with MDR1 and MRPs [195], additional studies will be required to define the relative contribution of each of these transporters in the overall and tis-... 

Shaheen RM, Davis DW, Liu W, et al. Antiangiogenic therapy targeting the tyrosine kinase receptor for vascular endothelial growth factor receptor inhibits the growth of colon cancer liver metastasis and induces tumor and endothelial cell apoptosis. Cancer Res 1999 59(21) 5412-5416. 

Upon coverage of longitudinally cleaved tubes with bovine endothelial cells in cell culture tests, a distinct sprouting of the initially spherical cells takes places within 24 h. SEM shows that the resulting filaments barely differ from the fibers of BC (Fig. 21). This structure also benefits the rapid endothelial colonization of BASYC . 

P-gp is constitutively expressed in nearly all barrier tissues. Techniques involving Northern blots (37) or Western blots with monoclonal antibodies such as C219 (38) and MRK 16 (39) have been used extensively to determine the tissue distribution of P-gp. It is expressed in adrenal cortex, kidney, liver, intestine, and pancreas endothelial cells at blood-tissue barriers, namely, the CNS, the testis, and in the papillary dermis (3,4,38,40,41). P-gp displays specific subcellular localization in cells with a polarized excretion or absorption function. More specifically, P-gp is found at the apical (AP) canalicular surface of hepatocytes, in the AP membrane of the columnar epithelial cells of colon and jejunum, and the AP brush border of the renal proximal tubule epithelium (3,4,40 1-2). In endothelial cells, P-gp is located in the luminal membrane (4,43). 

In in vivo studies, sorafinib (1) demonstrated efficacy in multiple cancer models. It inhibited the tumor growth of human melanoma renal, colon, pancreatic, hepatocellular, thyroid, and ovarian carcinomas and NSCLC, presumably through its anti-angiogenic effect on tumor endothelial cells that provide nutrients and help tumor growth. In addition, sorafenib (1) was shown to induce substantial tumor regression in a... 

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