Ehrlich-Ascites carcinoma cells

Elephantopus mollis is interesting because it elaborates a series of cytotoxic antitumor germacranolides including molephantinin and phantomolin, which are cytotoxic in vitro and in vivo against Ehrlich ascites carcinoma and Walker 256 carcinosarcoma in rodents (104,105). Molephantinin mitigates DNA and protein synthesis in Ehrlich ascites carcinoma cells and DNA synthesis. What is the activity of molephantinin on apoptosis (106)1... 

Ehrlich ascites carcinoma cell <21> (<21> from intraperitoneal cavity [70]) [70]... 

Mukherjee, K. Ghosh, S. Ray, M. Ray, S. Purification and characterization of 3-phosphoglycerate kinase from Ehrlich ascites carcinoma cells. Indian J. Biochem. Biophys., 39, 332-341 (2002)... 

Amounts of Glucose by Ehrlich Ascites Carcinoma Cells, Cancer Res. (1966) 26,276. 

May hew, E., Electrophoretic Mobility of Ehrlich Ascites Carcinoma Cells... 

Panfuran hydrochloride stimulates the central nervous system without causing paralysis. If excessive doses are administered, it causes anxiety, convulsions and stiffness shortly before death in frogs. Small dosages increase the blood pressure of rabbits, finally depression of blood pressure occurs however, panfuran haemolyses the red cells of rabbits in vitro and completely inhibits the growth of Ehrlich ascites carcinoma cells in vitro. Guanofuracin hydrochloride contracts blood vessels, and therefore temporarily raises the blood pressure. 

In order to clarify the mechanism of action of these compounds, their action suppressing the dehydrogenase of Ehrlich ascites carcinoma cells, and the syntheses of nucleic acids and protein by coli bacilli was examined. It was presumed from the results that the antitumour action of the compound LXII) is mainly due to the suppression of dehydrogenase action and that of compound LXIII) and compound LXIV) is due to the suppression of dehydrogenase action and syntheses of deoxyribonucleic acid, ribonucleic acid, and protein in tumour cells. 

Simg, C.-P. Johnstone, R.M. Phosphorylation of choline and ethanolamine in Ehrlich ascites-carcinoma cells. Biochem. J., 105, 497-503 (1967)... 

Experiments with Ehrlich-Ascites carcinoma cells led to the conclusion that inhibition of mitochondrial respiration is the mode of action of strobilurin and... 

Mukherjee KL, Heidelberger C (1962) Studies of fluorinated pyrimidines. XV. Inhibition of the incorporation of formate-C14 into DNA thymine of ehrlich ascites carcinoma cells by 5-fluoro-2 -deoxyuridine-5 -monophosphate and related compounds. Cancer Res 22 815-822... 

Fig. 1-Sedimentogram of isolated nuclear D-RNA from Ehrlich ascites carcinoma cells labeled with 14C orotic acid for 45 minutes. D-RNA obtained by hot phenol fractionation in the temperature interval between 55 and 85 . (Georgiev and Mantieva, 1962 Arion, Mantieva and Georgiev, 1967). Ultracentrifugation in SW-25 rotor in 5 to 20 percent sucrose gradient in 0.5 x SSC - 0.005 M EDTA-Na, pH 7.5, for 12 hours at 21,000 rpm.

By the method described it is possible to extract D-RNA-containing particles from such tissues as rat liver, Ehrlich ascites carcinoma cells (Samarina et al., 1965a), mouse liver (Lukanidin, 1969), and KB cells (Koehler and Arends, 1968). An important modification of this method was introduced by Samarina et al. (1967e) who performed the extraction with STM at pH 8.0 in the presence of an RNase inhibitor from rat Ever supernatant (Roth, 1958 Shortman, 1961 Lawford et al., 1967). The introduction of the RNAase inhibitor somewhat reduces the yield of D-RNA-containing material (from 85 to 90 down to 70 to 80 percent), but it makes it possible to isolate complexes more like native structures (see p. 53) than by the former method. 

The presence of informosomelike RNPs has been demonstrated also in the case of Ehrlich ascites carcinoma cells infected with Sendai virus (Volkova et al., 1969). The cytoplasmic extracts of the cells labeled for 30 minutes with uridine contain virus-specific RNA in the form of particles with a sedimentation coefficient 45S (the sedimentation coefficient of the complete virus equals 57S). The buoyant density of the RNP peak in CsCI equals 1.43 to 1.44 g/cm. Although these properties are compatible with the idea that they are informosome, the particles, and in particular their protein component, should be characterized in more detail before reaching a definite conclusion. Recently SOS virus RNA-containing particles with p = 1.40 g/cm have been found in HeLa cells infected with poliovirus (Huang and Baltimore, 1970), although the authors have some doubts about the reality of these complexes. 

Benade. L., Howard, T., and Burk, 0. Synergistic killing of Ehrlich ascites carcinoma cells by ascorbate and 3-amino 1.2,4-triazole. Oncology. 23. 33-43,1969. 

Benade, L. E. Ascorbate toxicity in Ehrlich ascites carcinoma cells. Ph.O. Dissertation, George Washington University, St. Louis, Mo.. 1971. 

Ehrlich ascites carcinoma cells implanted intra-peritoneally in female mice were reduced in their viability and volume by oral supplementation of melatonin at 50mg/kg body weight (El-Missiry and El-Aziz 2000). Flow cytometric studies showed that melatonin not only delayed the progression of cells from GO/Gl phase to S-phase of the cell cycle but also reduced DNA synthesis during cell cycle. In addition, the aneuploidy status was depressed in melatonin treated mice. 

Peirce, K., Abe, T., and Cooper, B.A., 1975. Incorporation and metabolic conversion of cyanocobalamin by Ehrlich ascites carcinoma cells in vitro and in vivo. Biochimica et Biophysica Acta. 381 348-358. 

Chen QH, Liu CY, Qiu CH 1980 Studies on Chinese rhubarb. XII. Effect of anthraquinone derivatives on the respiration and glycolysis of Ehrlich Ascites Carcinoma Cell. Yao Hsueh Hsueh Pao 15 65-70... 

Fan YJ, Zhou J, Li M 1981 Effect of nitidine chloride on the life cycle of Ehrlich Ascites carcinoma cells in mice. Chung-Kuo Yao Li Hsueh Pao 2 46-49... 

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