Drosophila cell line

Clemens, J.C. et al. 2000. Use of double-stranded RNA interference in Drosophila cell lines to dissect signal transduction pathways. Proc. Natl. Acad. Sci. USA 97, 6499-6503. 

The NOV form of the virus was also used to infect a Drosophila cell line in vitro. Flies are not part of the host range of this particular baculovirus and no progeny virus were anticipated. The situation with regard to gene... 

Most cellular signaling data associated with protein turnover have been collected in Drosophila, cell lines, and rodents. In the forthcoming paragraphs we will briefly summarize the literature with the latest understanding of the ceUular and molecular mechanisms associated with human age-related muscle wasting in response to nutrition and exercise, then detail potential therapeutic interventions that may counteract age-related muscle loss. 

Most investigators use Drosophila cell lines as hosts for transformation experiments. The goal may be to characterize a promoter, to investigate the role of a transcription factor, to overexpress a polypeptide, or to do something more novel. Figure 20.1 illustrates two novel and effective uses of stably transformed S2 cells. 

There is, as yet, no central repository for Drosophila cell lines. S2 and Kc cells are widely used obtaining starter cultures should present no problems. (Be aware, however, that... 

Karotype analysis. For karyotype analysis, we have used Protocol 20.2 successfully for a number of Drosophila cell lines. For an alternative protocol, see Echalier (1997). 

Cell cultivation on polylysine-coated surfaces. Most commonly used Drosophila cell lines adhere only loosely to the substrate. For some purposes (e.g., time-lapse photography and cytological examination of living cells), it is desirable to fix the growing cells more firmly to the substrate. This can be accomplished by treating the substrate with polylysine, with no obvious effect on the growth of the cells (see Protocol 20.3). 

In our experience, any Drosophila cell line can be cloned by limiting dilution (in 96-well plates) provided the growth of isolated cells is supported by the presence of a suspension of X-rayed feeder cells. Some fines (notably S2) can be cloned in soft agar, at a considerable saving of labor and money. Detailed procedures for both techniques have been published recently (Cherbas et al. 1994 Cherbas and Cherbas 1998). 

A recent and exciting development is the demonstration by C.A. Worby and J.E. Dixon (Biological Chemistry, University of Michigan) that RNAi effectively abolishes expression of genes in several Drosophila cell lines. They have developed a simple protocol for its use and report success in blocking expression of each (of 10) signal transduction pathway gene tested. 

Cherbas L., Moss R., and Cherbas P. 1994. Transformation techniques for Drosophila cell lines. Methods Cell Biol. 44 161-179. 

Savakis C., Demetri G., and Cherbas P. 1980. Ecdysteroid-inducible polypeptides in a Drosophila cell line. Cell 22 665-674. 

Cullen CF, Milner MJ 1991 Parameters of growth in primary cultures and cell lines established from Drosophila imaginal discs. Tissue Cell 23 29—39 Davis KT, Shearn A 1977 In vitro growth of imaginal disks from Drosophila melanogaster. Science... 

R aff I wonder if the reason that there are relatively few cell lines in flies is that if they don t make IDGFs you don t get them as lines. Have you looked to see whether all Drosophila cell fines make IDGFs ... 

Most of the viral vectors were constructed using (1) the Autographa californica nuclear polyhedrosis virus (AcNPV), which is able to infect moth species, Spodoptera frugiperda ovarian cell lines and, in specific conditions, Drosophila cells (2) the Bombyx mori nuclear polyhedrosis virus (BmNPV), which is able to infect silkworm cells. To broaden the range of infection of hosts, a hybrid virus was generated [118,119]. 

K. Archipelago regulates Cydin F levels in Drosophila and is mutated in human cancer cell lines. Nature 2001,... 

Moberg, K. H., Bell, D. W., Wahrer, D. C., Haber, D. A., and Hariharan, I. K. (2001). Archipelago regulates Cyclin E levels in Drosophila and is mutated in human cancer cell lines. Nature 413, 311-6. 

The S2 cell line, isolated from late stage fruit fly Drosophila melanoga-ster embryos, was established in 1969 by Schneider, from hundreds of 20-24-hour-old embryos (Schneider, 1972). This cell line can be genetically modified and is capable of stably expressing heterologous proteins, being easily adapted to suspension growth. 

Schneider I (1972), Cell lines derived from late embryonic stages of Drosophila melanogaster,]. Embryol. Exp. Morphol. 27 353-365. 

Drosophila melanogaster imaginal disc cell lines... 

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