Polylysine coating

Figure C 1.5.6. Single Ag nanoparticles imaged with evanescent-wave excitation. (A) Unfiltered photograph showing scattered laser light (514.5 nm) from Ag particles immobilized on a polylysine-coated surface. (B) Bandpass filtered (540-580 nm) photograph taken from a blank Ag colloid sample incubated witli 1 mM NaCl and...

Potential applications of vitamin Bi2 in electrocatalytic degradation of dibromide and a-haloacetic acid pollutants has been demonstrated in aqueous buffers303,304 and in surfactant-stabilized emulsions.305 Electroreductive dehalogenations in water and microemulsions were also efficiently catalyzed by a vitamin Bi2 derivative grafted onto a polylysine-coated electrode.306... 

This flattened erythrocyte preparation has been used to study reversible nonspecific adsorption kinetics and surface diffusion of insulin on the external surface of erythrocytes.(123) The nonspecific adsorption of insulin to the polylysine-coated substrate is very large compared to the adsorption to the flattened membrane adhered to the substrate. Fortunately, this nonspecific background fluorescence can be very successfully quenched simply by preparing the polylysine coating on an aluminum-film-coated glass surface, rather than on bare glass. As discussed in Section 7.3, the aluminum abolishes the fluorescence of fluorophores adsorbed directly onto the polylysine substrate, but the fluorophores adsorbed to the erythrocyte surface are not substantially quenched, because they are spaced at least two membrane thicknesses away. 

Bussiek, M., Mficke, N., and Langowski, J. (2003) Polylysine-coated mica can be used to observe systematic changes in the supercoiled DNA conformation by scanning force microscopy in solution. Nucleic Acids Res. 31, 137. 

Cut 5-6 pm thick sections of frozen tumors with a Cryostat using standard procedures, and mount onto polylysine-coated glass slides... 

The work of Larson et al. (62) represented the first detailed study to show agreement between AFM-derived diffuse layer potentials and ((-potentials obtained from traditional electrokinetic techniques. The AFM experimental data was satisfactorily fitted to the theory of McCormack et al. (46). The fitting parameters used, silica and alumina zeta-potentials, were independently determined for the same surfaces used in the AFM study using electrophoretic and streaming-potential measurements, respectively. This same system was later used by another research group (63). Hartley and coworkers 63 also compared dissimilar surface interactions with electrokinetic measurements, namely between a silica probe interacting with a polylysine coated mica flat (see Section III.B.). It is also possible to conduct measurements between a colloid probe and a metal or semiconductor surface whose electrochemical properties are controlled by the experimenter 164-66). In Ref. 64 Raiteri et al. studied the interactions between... 

Fig. 11.3. Evaluation of stretching uniformity, (a) Histogram constructed from the end-to-end length of seventy T7-DNA-RNAP-QD complexes. A Gaussian fit to the data is centered at 11.75 xm corresponding to 86% of the contour length. The width of the distribution is 1.6 pm corresponding to about 12%. (b) Cropped fluorescence images of fuU length T7-phage genomes flow stretched on a polylysine coated coverslip...

Adsorption differences between ligand and binding protein (coated glass fiber filters, nitro-cellulose/PVDF membranes, polylysin-coated microtiter plates)... 

A related technique adsorbs the binding protein to polylysine-coated microtiter plates instead of dotting it on nitrocellulose. After blocking the unsaturated polylysine, the wells are incubated with radioactive ligand (Scheer and Meldolesi 1985). 

The colloidal partides were immobilized on charges on the particles and lhe positive charges polylysine coated glass surfaces by the electro- on lhe surface, static interactions between the negative... 

OTHER COAT MATERIALS FOR ALGINATE MICROCAPSULES 33.7.1 POLYLYSINE-COATED AlCINATE MiCROCAPSULES... 

The literature reports show an improvement in survival and stability of microencapsulated probiotic bacteria during exposure to harsh acid conditions when polylysine has been used as a coating material. Several studies reported on alginate-polylysine microencapsulation systems coupled with bifidobacteria to investigate their survival in vitro and in vivo after oral administration. After 16 weeks of storage at 4°C, the stability of polylysine-coated alginate microcapsules successfully maintained the survival of bifidobacteria at over lO cfu/g. ... 

Cell cultivation on polylysine-coated surfaces. Most commonly used Drosophila cell lines adhere only loosely to the substrate. For some purposes (e.g., time-lapse photography and cytological examination of living cells), it is desirable to fix the growing cells more firmly to the substrate. This can be accomplished by treating the substrate with polylysine, with no obvious effect on the growth of the cells (see Protocol 20.3). 

High concentrations of protein in the medium can inhibit cells from adhering tightly to polylysine-coated surfaces. If this is a problem, allow cells to adhere to the treated surface in serum-free medium for 1-2 hours, and then replace medium with the normal growth medium. 

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