Dissolved oxygen control systems

This Is a compilation of articles and papers from WEFJournals and conferences. Topics Include toxicity reduction evaluations, computerized controllers, information systems, data management, analytical procedures, dissolved oxygen control, respirometers, and control methods. 

The function of aeration in a wastewater treatment system is to maintain an aerobic condition. Water, upon exposure to air, tends to estabUsh an equihbrium concentration of dissolved oxygen (DO). Oxygen absorption is controlled by gas solubiUty and diffusion at the gas—hquid interface. Mechanical or artificial aeration may be utilised to speed up this process. Agitating the water, creating drops or a thin layer, or bubbling air through water speeds up absorption because each increases the surface area at the interface. 

The production of hydroxide ions creates a localized high pH at the cathode, approximately 1—2 pH units above bulk water pH. Dissolved oxygen reaches the surface by diffusion, as indicated by the wavy lines in Figure 8. The oxygen reduction reaction controls the rate of corrosion in cooling systems the rate of oxygen diffusion is usually the limiting factor. 

A hybridoma can live indefinitely in a growth medium that includes salts, glucose, glutamine, certain amino acids, and bovine serum that provides essential components that have not been identified. Serum is expensive, and its cost largely determines the economic feasibihty of a particular ciilture system. Only recently have substitutes or partial replacements for serum been found. Antibiotics are often included to prevent infection of the culture. The pH, temperature and dissolved oxygen, and carbon dioxide concentration must be closely controlled. The salt determines the osmotic pressure to preserve the integrity of the fragile cell. 

Activities associated with bioreactors include gas/hquid contacting, on-hne sensing of concentrations, mixing, heat transfer, foam control, and feed of nutrients or reagents such as those for pH control. The workhorse of the fermentation industry is the conventional batch fermenter shown in Fig. 24-3. Not shown are ladder rungs inside the vessel, antifoam probe, antifoam system, and sensors (pH, dissolved oxygen, temperature, and the like). Note that coils may lie between baffles and the tank wall or connect to the top to minimize openings... 

Bioprocess Control An industrial fermenter is a fairly sophisticated device with control of temperature, aeration rate, and perhaps pH, concentration of dissolved oxygen, or some nutrient concentration. There has been a strong trend to automated data collection and analysis. Analog control is stiU very common, but when a computer is available for on-line data collec tion, it makes sense to use it for control as well. More elaborate measurements are performed with research bioreactors, but each new electrode or assay adds more work, additional costs, and potential headaches. Most of the functional relationships in biotechnology are nonlinear, but this may not hinder control when bioprocess operate over a narrow range of conditions. Furthermore, process control is far advanced beyond the days when the main tools for designing control systems were intended for linear systems. 

Oxygen is almost always a contributing factor to corrosion mechanisms therefore, the effective removal of dissolved oxygen (DO) is of paramount importance in controlling the rate of boiler system corrosion, irrespective of the size, design, or pressure of the plant. 

Conclusions - Dissolved Oxygen. Continued attainment of DO standards in the Willamette Basin in the face of a current regional growth rate of 1% yr will require continued augmentation of flow as well as pollution control, particularly with respect to ammonia. Based on model results discussed, there appears to be little justification for the installation of advanced wastewater treatment systems in the basin for the purpose of maintaining acceptable DO levels. 

Even if few systems are proposed for inorganic compounds (with regard to the number of potential pollutants), instruments or sensors for parameters used for treatment process control are available UV systems for residual chlorine in deodorization, electrochemical sensors for dissolved oxygen (with nowadays a luminescent dissolved-oxygen probe utilizing a sensor coated with a luminescent material) and a colorimetric technique for residual ozone. 

An example of CQV of the batch cultivation of a vaccine has been demonstrated, where univariate (temperature, dissolved oxygen, pH) as well as spectroscopic tools were used to develop process models. The measurements were used for a consistency analysis of the batch process, providing better process understanding which includes the understanding of the variations in the data. MSPC analysis of four batches of data was performed to monitor the batch trajectories, and indicated that one batch had a deviation in the pH. From the MSPC information, combined with calibration models for the composition of the process based on NIR spectral data, improved monitoring and control systems can be developed for the process, consistent with concept of CQV. The data from the univariate sensors and NIR were also fused for a global analysis of the process with a model comprised of all the measurements. 

A bioprocess system has been monitored using a multi-analyzer system with the multivariate data used to model the process.27 The fed-batch E. coli bioprocess was monitored using an electronic nose, NIR, HPLC and quadrupole mass spectrometer in addition to the standard univariate probes such as a pH, temperature and dissolved oxygen electrode. The output of the various analyzers was used to develop a multivariate statistical process control (SPC) model for use on-line. The robustness and suitability of multivariate SPC were demonstrated with a tryptophan fermentation. 

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