Dissociation of tissue

G6. Glass, C., Pittman, R. C., Weinstein, D. B., and Steinberg, D., Dissociation of tissue uptake of cholesterol ester from that of apoprotein A-I of rat plasma high density lipoprotein selective delivery of cholesterol ester to liver, adrenal, and gonad. Proc. Natl. Acad. Sci. U.S.A. 80, 5435-5439 (1983). 

Heterogeneous reaction (Section 6 1) A reaction involving two or more substances present in different phases Hydro genation of alkenes is a heterogeneous reaction that takes place on the surface of an insoluble metal catalyst Heterolytic cleavage (Section 4 16) Dissociation of a two electron covalent bond in such a way that both electrons are retained by one of the initially bonded atoms Hexose (Section 25 4) A carbohydrate with six carbon atoms High density lipoprotein (HDL) (Section 26 11) A protein that carries cholesterol from the tissues to the liver where it is metabolized HDL is often called good cholesterol Histones (Section 28 9) Proteins that are associated with DNA in nucleosomes... 

Stimulation and inhibition of the enzyme by the GPCR-G-protein cycle occur by analogous mechanisms. Agonists induce hormone receptors to increase a Ga-GDP-GTP exchange and subsequent Ga 3y dissociation (GDP-a py + GTP GTP-ax + [3y + GDP) (Fig. 4). Consequently, agents that affect either the dissociation of either G or Gs, or the association of their respective as, a , or (3y subunits with adenylyl cyclase could affect rates of cAMP formation in enzyme preparations or in intact cells and tissues. There are several important examples. Gas is stably activated by poorly hydrolyzable analogs of GTP, e.g. GTPyS... 

Drought also has a profound effect on protein synthesis. In many plant tissues, a reduced water potential causes a reduction of total protein synthesis and a rapid dissociation of polyribosomes. The latter has been shown not to be the consequence of increase in ribonuclease activity (Hsiao, 1973 Dhindsa Bewley, 1976). For a specific protein, Jacobsen, Hanson Chandler (1986) have shown in barley leaves that water stress enhances the synthesis of one of the a-amylase isozymes. Using a cDNA probe they found that water-stressed leaves contained much more a-amylase mRNA than unstressed plants. 

The free fatty acid uptake by tissues is related directly to the plasma free fatty acid concentration, which in turn is determined by the rate of lipolysis in adipose tissue. After dissociation of the fatty acid-albumin complex at the plasma membrane, fatty acids bind to a membrane tty acid transport protein that acts as a transmembrane cotransporter with Na. On entering the cytosol, free fatty acids are bound by intracellular fatty acid-binding proteins. The role of these proteins in intracellular transport is thought to be similar to that of serum albumin in extracellular transport of long-chain fatty acids. 

The biosynthesis of Me3,Mel 1-29 H takes place in oenocyte cells, released into the hemolymph and transported by lipophorin to peripheral tissues (Fig. 7) [71, 231, 232]. Direct evidence for oenocytes biosynthesizing hydrocarbon has come recently with the dissociation of oenocytes from epidermal cells and in vitro incubation with labeled propionate [233]. Differential uptake of some hydrocarbons in different tissues has also been documented although the exact mechanism behind the differential placement of hydrocarbons is unknown [20,128,230,232,234]. Although the biosynthesis of hydrocarbons may not be under direct endocrine regulation supply of precursor hydrocarbon that is converted to the sex pheromone is a requirement. 

In the past, dissociation of the nucleoprotein complex has been brought about by salt solutions or by heat denaturation,129 but, more recently, decomposition has been effected by hydrolysis with trypsin,126 or by the use of dodecyl sodium sulfate130 or strontium nitrate.131 Some virus nucleoproteins are decomposed by ethyl alcohol.132 This effect may be similar to that of alcohol on the ribonucleoproteins of mammalian tissues. If minced liver is denatured with alcohol, and the dried tissue powder is extracted with 10% sodium chloride, the ribonucleoproteins are decomposed to give a soluble sodium ribonucleate while the deoxyribonucleoproteins are unaffected.133 On the other hand, extraction with 10 % sodium chloride is not satisfactory unless the proteins have first been denatured with alcohol. Denaturation also serves to inactivate enzymes of the tissues which might otherwise bring about degradation of the nucleic acid during extraction. 

Manganese(II)-A/, A/r -dipyridoxylethylenediamine-A/r, AT-diacetate 5,5 -bis(phosphate) 75 (DPDP) is clinically used for enhancing contrast in the liver (detection of hepatocellular carcinomas) (312). Some dissociation of Mn(II) appears to occur in the liver, and enhancement can also be obtained in functional adrenal tissues (313). Manganese(II)-tetrasulfonated phthalocyanine also shows tumor localization properties and is a more efficient relaxation agent than the analogous Gd(III) complexes (314). 

Prior to immunohistochemical staining, paraffin sections must be properly mounted onto slides, and then deparaffinized and rehydrated. To help adherence to the glass and decrease the chances of sections dissociating from the slides, paraffin tissue sections should be mounted on tissue-adhesive-coated slides. The use of tissue-adhesive-coated slides is especially important for paraffin tissue sections undergoing heat-induced antigen retrieval (see Sect. 6.1.1). 

The enzymatic method (5,6) is probably the method of choice as a starting point for most tissue types because of its ability not only to release a large number of cells, but also to preserve cellular integrity and viability (7). Last, chemical dissociation is commonly used in conjunction with mechanical or enzymatic procedures. Chemical methods are designed to omit or sequester the Ca + and Mg + ions needed for maintenance of the intercellular matrix and cell-surface integrity. Ethylenediaminoacetate (EDTA) or citrate ion is commonly used to remove these cations, but does not adequately dissociate all types of tissue. 

Dissociation of Digested Neural Tissue and Primary Culture... 

Conditions in the peripheral tissues that stabilize the structure of deoxyhemoglobin promote dissociation of O2. 

Weit Joe Takahashi, is your expectation that you would only see this at the tissue level The idea is presumably that in a cell-autonomous sense, period and phase are still coupled because of the way we imagine the oscillator works, but when you are now talking about how a collection of coupled oscillators integrate this into behavioural outputs, there are now other factors that must come into play. Is this fair to say Is it only in the chimeras, and not in the heterozygotes, that you see these unusual combinations and dissociations of phase and period ... 

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