Disinfection work surfaces

Frequently disinfect working surfaces with bactericide or germicide. Disinfect hands with dilute germicidal soap. 

Aseptic techniques are used to avoid the possibility of infection of the animals or ceU cultures. These include the preparation of the vaccines and spleens under aseptic conditions in a class 100 clean room equipped with a laminar airfiow hood, sterilization of instruments, and treatment of work surfaces with disinfectant before and after use, washing of the investigator s hands with an antiseptic surgical scrub preparation, and wearing of sterile gloves, face mask, and eyeglasses. 

In the event of cell cultures becoming contaminated with bacteria, fungi or mycoplasmas, the best course of action is to discard the culture, check cell culture reagents for contamination, thoroughly disinfect all safety cabinets and work surfaces and resuscitate a fresh culture from previously frozen stock. In the case of contamination with a spore-forming organism, and where such facilities exist, room fumigation may also be advisable. 

Laboratory equipment and work surfaces are cleaned with an appropriate disinfectant after work is finished and decontaminated immediately after spills or other contamination by infectious materials. All accidents or incidents must be immediately reported to the laboratory director, medical evaluation provided, and written records maintained. 

With the exception of activated carbon, not much work has been reported on the reactions of disinfectants at surfaces of the types expected to be encountered in water treatment, although reactions of free chlorine with the organic matter at the surfaces of several natural sediments have been shown to produce trihalomethanes (Uhler and Means, 1985). Activated carbons have long been known to be potent catalysts of redox (cf. Austin et al., 1980) and other reactions such as eliminations... 

Work surfaces shaft be decontaminated with an appropriate disinfectant (i) after completion of procedures, (ii) immediately or as soon thereafter as feasible when surfaces are overtly contaminated, (iii) after any spill of blood or potential infectious materials, or (iv) at the end of the work shift if the surface may have been contaminated since the last cleaning. 

Laboratory equipment and work surfaces should be decontaminated with an appropriate disinfectant on a routine basis, after work with infectious materials is finished, and especially after any overt spills, splashes, or other contamination by infectious materials. Contaminated equipment must be decontaminated before it is sent for repair or maintenance or packaged for transport in accordance with applicable local, state, or federal regulations before removal from the facility. 

Work surfaces must be cleaned with an appropriate disinfectant. 

There is an important difference between cleaning and disinfection. Cleaning is removing dirt and other unwanted substances. Disinfection is aimed at reducing germs on surfaces (e.g. worktops) and is effective mily when the surfaces are cleaned beforehand (see also Sect. 31.4). In practice, the right order is first cleaning and then, where necessary (for example, the work surface of a laminar flow cabinet), disinfection. 

The Centers for Disease Control state that HBV can survive for at least one week in dried blood on surfaces so it s very important to clean and sanitize properly. Contaminated work surfaces must be decontaminated with a disinfectant following an emergency incident. Take into consideration the appropriate methods of decontamination based upon the location within the facility, type of surface, types of contamination, if any, and tasks or procedures being performed. 

What type of disinfectant can be used to decontaminate equipment or working surfaces which have come in contact with blood or other potentially infectious materials (OPIM) ... 

Disinfecting Work Areas Since contamination can spread so easily and inadvertently, when handling potentially infectious agents, such as blood, it is important to frequently clean and disinfect the work surfaces where these materials might be used. A 1% dilution of common household bleach (5% sodium hypochlorite) is an effective disinfectant - these solutions must be made daily. Equipment that may be potentially contaminated with blood should also be wiped down to ensure disinfection. Not all bacteria or viruses can be inactivated by exposure to chlorine (bleach) so it is best to seek information about the correct disinfectant for the agents in use. Public Health Agency of Canada has developed SDSs for infectious agents and these SDSs contain information about disinfection and laboratory safety. ... 

Appropriate disinfectant should be applied following any spill of potentially infectious materials or at the end of daily work to decontaminate laboratory work surfaces. Spill kits (containing absorbent pads and/or neutralizing agents) should be prepared for all frequently used chemicals or biological waste. 

Are proper disinfectants that kill pathogens used to clean working surfaces, bins, or other areas where contamination may occur ... 

When pipets are used, avoid accidentally dropping infectious cultures from the pipet. Place a disinfectant-soaked towel on the working surface and autoclave the towel after use. 

The most convenient way to disinfect work areas and materials, equipment, and instruments is through the use of chemical agents. Use of chemical disinfection is often necessary because pressurized steam, the most reliable method of sterilization, is not feasible for disinfecting large spaces, surfaces, and large items of equipment. Moreover, high temperatures and moisture can damage delicate instruments, particularly those with optical and electronic components. 

The worker then must disinfect the interior work surfaces of cabinet with 70 percent ethanol in water. An absorbent, plastic-lined bench pad should be placed in the cabinet, taking care not to cover the front and rear air intake and exhaust grilles. 

Particular attention must be paid to disinfection of work surfaces before, after, and at intervals during work in the laboratory. Thorough wiping with 70 percent ethanol should be the minimum requirement. 

Whithin the Horizontal Working Group (HWG) a Task Group about Surface Disinfection was built by experts from France, UK, Belgium and Germany, the Surface Task Group (STG). 

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