Diploids

Composition and Methods of Manufacture. Vaccine is produced from the Oka attenuated strain. Vacciae is produced in human diploid cells such as MRC-5. After growth in the cell substrate, the cells themselves are harvested into the growth medium and sonicated to release the cell-associated vims. Sucrose and buffering salts are generally in the medium to help stabiLize the vims. The vacciae is presented in a free2e-dried vial to be reconstituted with sterile distilled water before injection (27). 

There is one nncleosome for every 200 bp of eukaryotic DNA. How many nncleosomes are in a diploid human cell Nncleosomes... 

Diploeder, n, (Cryst.) diplohedron, diploid, dipol, a. dipolar. 

Imovax Rabies I.D. Vaccine (human diploid cell), Imovax Rabies Vaccine (human diploid cell), Rab Avert,... 

The cytoskeleton undergoes extensive reorganization during mitosis, and is responsible for the equipartition of a diploid set of chromosomes to each daughter cell (McIntosh and Koonce, 1989 Wadsworth, 1993). 

Vitamins and lipids are often required for animal cells to grow in serum-free medium. Phosphoethanolamine and ethanolamine are key additives that facilitate the growth of the mammary tumor cell line 64024 (Kano-Sueoka and Errick, 1981). In addition, ethanolamine promotes the growth of human lymphocytes and mouse hybridoma cells. Short-term cultures of human diploid lung and foreskin fibroblasts grow in medium that includes among its supplements soybean lecithin, cholesterol, sphingomyelin, and vitamin E. 

In diploid eukaryotic organisms such as humans, after cells progress through the S phase they contain a tetraploid content of DNA. This is in the form of sister chromatids of chromosome pairs. Each of these sister... 

Fig. 2.4 The budding pattern in haploid and diploid Saccharomyces cerevisiae. The original cell which formed a bud is the mother (M). The daughter cell (D) is shown remaining attached as might be the case in i colonies growing on the surface of agar.

Hepatitis At Human diploid cells infected with hepatitis A virus 1 Separation of virus from cells 2 Inactivation with HCHO 3 Adsorption toAI(OH)3gel Assay of antigen content by ELISA Inoculation of cell cultures to exclude presence of live virus... 

Poliomyelitis (inactivated)t (Salktype) Human diploid cell cultures infected with each of the three serotypes of poliovirus 1 Clarification 2 Inactivation with formalin 3 Concentration 4 Blending of virus of each serotype Induction of antibodies to polioviruses in chicks or guinea-pigs Inoculation of cell cultures and monkey spinal cords to exclude live virus... 

Rubella (German measles) Human diploid cell cultures infected with attenuated rubella virus 1 Clarification 2 Blending with stabilizer 3 Freeze-drying Infectivity titration In cell cultures Tests to exclude presence of extraneous viruses... 

A more general example fiom virus vaccine produetion is the rigorous examination of tissue cultures to exclude contamination with infectious agents fiom the sonrce animal or, in the cases of human diploid cells or cells fiom eontinuous cell lines, to detect... 

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