Diploid population

Excoffier L, Slatkin M. Maximum-likelihood estimation of molecular haplotype frequencies in a diploid population. Mol Biol Evol 1995 12 921-927. 

Clark AG. Inference ofhaplotypes from PCR-amplified samples of diploid populations. Mol Biol Evol 1990 7 111-122. 

The ability of some fluorescent dyes to bind DNA quantitatively is exploited in flow cytometry to determine the DNA content of a cell. Dyes such as propidium iodide that bind double-stranded DNA stoichiometrically can be used for the purpose. The intensity of red fluorescence is directly related to the amount of DNA bound by propidium iodide. By comparing the fluorescence intensity of the test specimen and, in turn, its DNA content to the fluorescence intensity of specimens containing normal diploid amounts of DNA, a DNA histogram can be generated. By computing a DNA index, which is the ratio of DNA content of a test specimen to the DNA content of a specimen containing a normal diploid population, information related to the presence of an aneuploid tumor population can be obtained. The DNA index of 1 would imply that the DNA in the test specimen is from a normal diploid population (2N DNA), whereas the DNA index of an aneuploid population will be greater or less than 1. Thus, the DNA index of a tetraploid (4N DNA) would be 2. 

Cell sort populations according to cell cycle profile. Isolate diploid populations from diploid Gl and tetraploid populations from tetraploid G2 (see Note 2). 

Celis-Gamboa, C., Struik, P. C., Jaeobsen, E., Visser, R. G. F. (2005). Sprouting of seed tubers during cold storage and its influenee on tuber formation, flowering and the duration of the life eyele in a diploid population of potato. Potato Research, 46(1-2), 9-25. 

Van der Strate H, Van de Zande L, Stam WT, Olsen JL (2002) The contribution of haploids, diploids and clones to fine-scale population structure in the seaweed Cladophoropsis membranacea (Chlorophyta). Mol Ecol 11 329-345 Verlaque M, Boudouresque CF, Meinesz A, Gravez V (2000) The Caulerpa racemosa complex (Caulerpales, Ulvophyceae) in the Mediterranean sea. Bot Mar 43 49-68 Vinueza LR, Branch GM, Branch ML, Bustamante RH (2006) Top-down herbivory and bottom-up El Nino effects on Galapagos rocky-shore communities. Ecol Monogr 76 111-131 Wahl M and Hay ME (1995) Associational resistance and shared doom effects of epibiosis on herbivory. Oecologia 102 329-340... 

The gene frequency measures the proportion of chromosomes that contain a specific allele. To continue the RFLP example given above, we wish to estimate the frequencies of alleles 1 and 2 in our population. Each individual with the 1-1 genotype has two copies of allele 1, and each heterozygote (1-2 genotype) has one copy of allele 1. Because each diploid somatic ceU contains... 

Rousselle-Bourgeois, F., Rousselle, P. (1992). Creation et seleetion de populations diploids de pomme de terre Solanum tuberosum L.). Agronomie, 12, 59-67. 

---