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Detection modes amperometric

Pulsed amperometric detection (PAD), introduced by Johnson and LaCourse (64, 65) has greatly enhanced the scope of liquid chromatography/electrochemistry (66). This detection mode overcomes the problem of loss of activity of noble metal electrodes associated with the fixed-potential detection of compounds such as carbohydrates, alcohols, amino acids, or aldehydes. Pulsed amperometric detection couples tlie process of anodic detection with anodic cleaning and cathodic reactivation of a noble metal electrode, thus assuring a continuously cleaned and active... [Pg.92]

To ensure that the detector electrode used in MEMED is a noninvasive probe of the concentration boundary layer that develops adjacent to the droplet, it is usually necessary to employ a small-sized UME (less than 2 /rm diameter). This is essential for amperometric detection protocols, although larger electrodes, up to 50/rm across, can be employed in potentiometric detection mode [73]. A key strength of the technique is that the electrode measures directly the concentration profile of a target species involved in the reaction at the interface, i.e., the spatial distribution of a product or reactant, on the receptor phase side. The shape of this concentration profile is sensitive to the mass transport characteristics for the growing drop, and to the interfacial reaction kinetics. A schematic of the apparatus for MEMED is shown in Fig. 14. [Pg.348]

Mayer, W. J. and Greenberg, M. S., A comparison of differential pulse and D. C. amperometric detection modes for the liquid chromatographic determination of oxalic acid, /. Chromatogr Sci., 17, 614, 1979. [Pg.276]

Samples are introduced into the capillary by either electrokinetic or hydrodynamic or hydrostatic means. Electrokinetic injection is preferentially employed with packed or monolithic capillaries whereas hydrostatic injection systems are limited to open capillary columns and are primarily used in homemade instruments. Optical detection directly through the capillary at the opposite end of sample injection is the most employed detection mode, using either a photodiode array or fluorescence or a laser-induced fluorescence (LIF) detector. Less common detection modes include conductivity [1], amperometric [2], chemiluminescence [3], and mass spectrometric [4] detection. [Pg.156]

Many published articles on HPLC-ECD refer to the use of one of three voltammetric detectors (amperometric, coulometric, or polarographic). More detailed information on principles and techniques of various electrochemical detection modes can be obtained from the recent book, Coulometric Electrode Array Detectors for HPLC (34). There are also two electrode array detectors, the coulometric electrode array system and the CoulArray detector, currently available. Both detectors offer the qualitative data of PDA and the extreme sensitivity of ECD (34). The... [Pg.785]

A dual EC detection employing amperometry/conductivity allows measurements of a wider range of analytes per separation (ionic via CCD mode and electroactive compounds via amperometric detection) has also been reported [73]. This scheme also gave two simultaneous signals for the same component that is both charged and electroactive. This is the only report in which both principles are combined. Most of the CE-EC microchips are based in the amperometric detection mode that is more deeply considered in next section. [Pg.836]

Amperometry is the most widely reported EC detection mode for CE microchips, which primarily relies on oxidation or reduction of elect-rochemically active species by applying a constant potential to a working electrode. The current is then monitored as a function of time. Since it is based on the redox reaction that occurs at the electrode surface, electrodes can be miniaturised without loss in sensitivity. The relevance of this simple technique is reported in several reviews [48,74], In this section, a general overview of the combination of this detection technique to CE microchips together with special sections for different amperometric techniques and electrode materials and types are considered. [Pg.837]

The conductivity detection mode measures the change in conductance in the solution between two electrodes caused by the introduction or removal of charged species. In the amperometric detection mode, on the other hand, compounds undergo oxidation or reduction reactions through the loss or gain, respectively, of electrons at the electrode surface. The electrical current arising from the electrons passed to or from the electrode is recorded and is proportional to the concentration of the analyte present. Figure 3.21 illustrates the difference between amperometry and conductivity.49... [Pg.102]

The three most common modes of operation of electrochemical detection are amperometric, coulometric, and potentiometric. An amperometric detector is an electrochemical cell that produces a signal proportional to the analyte concentration usually the percentage of the analyte that undergoes the redox reaction is very low, about 5%. [Pg.143]

In the detection modes applied to ion chromatography one distinguishes between electrochemical and spectroscopic methods. Conductometric and amperometric detection are electrochemical methods, while the spectroscopic methods embrace UV/Vis, fluorescence, and refractive index detection. Added to this are the various applications of these detection methods, described in detail below. [Pg.291]

Detection electrochemical amperometric detector LC-4B (Bioanalytical Systems Inc.) mode single electrode cell geometry thin layer, 2 pm working electrode glassy carbon reference electrode silver / silver chloride range 10 nA. [Pg.551]

The three basic detection modes of electrochemical detection are conductivity, amperometric, and potentiometric detection. [Pg.201]

Because many amines elute under chromatographic conditions that are suitable for the separation of alkali and alkaline earth metals, direct conductivity detection is possible. However, amperometric and UVWis detection modes are much more selective and sensitive. [Pg.1204]

Amperometric Detectors. Amperometric detectors are the most commonly used electrochemical detectors for highly sensitive and selective determinations in HPLC [30], [31 ]. The frequently used synonym electrochemical detector is not very precise in relation to the detection mode. [Pg.276]

Three different detection modes can be utilized including fixed potential preferred in flow systems and for biosensors, step pulse potential and sweeping potential of which the last two are preferred in batch systems. Up and coming are microchip capillary electrophoresis coupled with amperometric screen-printed electrodes [25], Metal sensors are also used, but lack selectivity. They are more useful for classification applications than for evaluation of taste, like predicting sensorial descriptors of Italian red dry wines of different origins [26]. [Pg.435]

Melanoma can be diagnosed throngh the monitoring of tyrosinase, a cytoplasmic melanocyte differentiation protein, which is a key enzyme in melanin synthesis and has been listed as important melanoma biomarker. Mossberg et al. (2014) developed an electrochemical biosensor platform with an amperometric detection mode to detect the enzymatic activity of tyrosinase in fresh biopsy samples withont pretreatment of the samples. The combination of this method with modem portable devices can provide interesting POC sensors in the fnture. [Pg.194]

Also using amperometric detection, Chen, et al. developed a three-dimensionally adjustable amperometric detector for MCE and applied to separate aromatic amines and nitroaromatic pollutants. Amperometry, and related pulse amperometric detection modes, are very attractive because provide enhanced selectivity related to conductimetric detection. [Pg.636]


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See also in sourсe #XX -- [ Pg.23 , Pg.28 , Pg.29 , Pg.33 , Pg.36 , Pg.39 ]




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