Capillary electrophoresis coupled with

In E. Coli bacterial lysates, the proteome (i.e., the full array of proteins produced) was analyzed by isoelectric focusing and mass spectrometry.97 A comparison of capillary electrophoretic separation and slab gel separation of a recombinant monoclonal antibody demonstrated that the precision, robustness, speed, and ease-of-use of CE were superior.98 Seventy-five proteins from the yeast ribosome were analyzed and identified by capillary electrophoresis coupled with MS/MS tandem mass spectrometry.99 Heavy-chain C-terminal variants of the anti-tumor necrosis factor antibody DE7 have been separated on capillary isoelectric focusing.100 Isoforms differing by about 0.1 pi units represented antibodies with 0,1 or 2 C-terminal lysines. 

Capillary electrophoresis coupled with a laser-induced fluorescence (LIF) detector has also been applied for the analysis of copper chlorophyll in olive oils. Samples were... 

Chakel, J. A., Erno, J., Hancock, W. S., and Swedberg, S. A. (1997). Analysis of recombinant DNA-derived glycoproteins via high-performance capillary electrophoresis coupled with off-line matrix-assisted laser desorption ionization time-of-fiight mass spectrometry. /. Chromatogr. B 689, 215—220. 

Sheppard, R. L., Tong, X., Cai, J., and Henion, J. D. (1995). Chiral separation and detection of terbutaline and ephedrine by capillary electrophoresis coupled with ion spray mass spectrometry. Anal. Chem. 67, 2054-2058. 

Wang, J., G. Chen, M. Chatrathi, K. Shin, and A. Fujishima. Microchip capillary electrophoresis coupled with a boron-doped diamond electrode-based electrochemical detector. Anal. Chem. 75, 935-939 (2003). 

Bianco, G., Schmitt-Kopplin, R, De Benedetto, G., Kettrup, A., and Cataldi, T. R. (2002). Determination of glycoalkaloids and relative aglycones by non-aqueous capillary electrophoresis coupled with electrospray ionization-ion trap mass spectrometry. Electrophoresis 23,2904—2912. 

K. Vuorensola, H. Siren and U. Karjalainen, Determination of dopamine and methoxycatecholamines in patient urine by liquid chromatography with electrochemical detection and by capillary electrophoresis coupled with spectrophotometry and mass spectrometry, J. Chromatogr. B, 788, 277-289 (2003). 

X.-P. Yan, X.-B. Yin, D.-Q. Jiang and X.-W. He, Speciation of mercury by hydrostatically modified electroosmotic flow capillary electrophoresis coupled with volatile species generation atomic fluorescence spectrometry, Ana/. Ghent., 75, 1726-1732, 2003. 

The presence of asymmetric carbons in selenomethionine, other a-selenoamino acids, and related compounds produces different chiral enantiomers with different physiological activities. HPLC separation of enantiomers is possible with a range of chiral stationary phases, d- and 1-Selenomethionine enantiomers have been resolved with an a-cyclodextrin stationary phase and other species with a teicoplanin-based chiral phase. Hybrid chiral methodologies based on GC, HPLC, and capillary electrophoresis, coupled with ICP-MS are feasible. Enantiomers of d,l-selenocystine, d,l-selenomethio-nine, and d,l-selenoethionine were examined in a range of commercial dietary supplements using a chiral crown ether stationary phase and ICP-MS detection. Selenium-em-iched onion, garlic, and yeast were analyzed and some of the selenoamino acid enantiomers were identified. l-Fluoro-2, 4-dinitro-phenyl-5-l-alanine amide was used to derivatize enantiomers of selenoamides for enhanced resolution. 

Yin XB, Qiu HB, Sun XH, Yan JL, Liu IF, Wang EK (2004) Capillary electrophoresis coupled with electrochemiluminescence detection using porous etched joint. Anal Chem 76(13) 3846-3850. doi 10.1021/ac049743j... 

Ding S-N, Xu J-J, Chen H-Y (2006) Microchip capillary electrophoresis coupled with an end-column electrochemiluminescence detection. Talanta 70(2) 403-407... 

Yin XB, Wang E (2005) Capillary electrophoresis coupling with electrochemiluminescence detection a review. Anal Chim Acta 533(2) 113-120. doi 10.1016/j.aca.2004.11.015... 

Deng B, Kang Y, Li X, Xu Q (2007) Determination of josamycin in rat plasma by capillary electrophoresis coupled with post-column electrochemiluminescence detection. J Chromatogr B 859(1) 125-130. doi 10.1016/j.jchromb.2007.09.014... 

Deng B, Shi A, Kang Y, Li L (2011) Determination of metformin hydrochloride using precolumn derivatization with acetaldehyde and capillary electrophoresis coupled with electrochemiluminescence. Lumin J Biol Chem Lumin 26(6) 592-597. doi 10.1002/ bio. 1276... 

Li X, Zhu D, You T (2011) Simultaneous analysis of six cardiovascular drugs by capillary electrophoresis coupled with electrochemical and electrochemiluminescence detection, using a chemometrical optimization approach. Electrophoresis 32(16) 2139-2147. doi 10.1002/elps.201100074... 

Yang R, Zeng H-J, Li J-J, Zhang Y, Li S-J, Qu L-B (2011) Capillary electrophoresis coupled with end-column electrochemiluminescence for the determination of ephedrine in human urine, and a study of its interactions with three proteins. Luminescence 26(5) 374-379. doi 10.1002/bio.l336... 

Deng B, Yin H, Liu Y, Ning X (2011) Pharmacokinetics of propranolol hydrochlorid in human urine by capillary electrophoresis coupled with electrochemiluminescence. Anal Sci 27(l) 55-59. doi 10.2116/analsci.27.55... 

Yuan B, Zheng C, Teng H, You T (2010) Simultaneous determination of atropine, anisodamine, and scopolamine in plant extract by nonaqueous capillary electrophoresis coupled with electrochemiluminescence and electrochemistry dual detection. J Chromatogr A 1217(1) 171-174. doi 10.1016/j.chroma.2009.11.008... 

Phillips, T.M. Smith, P. Analysis of intracellular regulatory proteins by immunoaffinity capillary electrophoresis coupled with laser-induced fluorescence detection. Biomed. Chromatogr. 2003,17, 182. 

J. Zhou, Y. Chen and R. Cassidy, Separation and determination of the macrolide antibiotics (erythromycin, spiramycin and oleandomycin) by capillary electrophoresis coupled with fast reductive voltammetric detection. Electrophoresis, 2000, 21, 1349-1353. 

Liu Q, Liu Y, Li Y, Yao S (2006) Nonaqueous capillary electrophoresis coupled with las -induced native fluorescence detection for the analysis of btaberine, palmatine, and jatFraihizine in Chinese herbal medicines. J Sep Sci 29 1268-1274. doi 10.1002/jssc.200600032 Liu Q, Liu Y, Guo M, Luo X, Yao S (2006) A simple and sensitive method of nonaqueous capillary electrophoresis with laser-induced native fluorescence detection fm the analysis of chelerythrine and sanguinarine in Chinese herbal medicines. Talanta 70 202-207. doi 10.1016/j.talanta.2006.02.049... 

Cai, J. and Henion, J. (1996a) Elucidation of LSD in vitro metabolism by liquid chromatography and capillary electrophoresis coupled with tandem mass spectrometry. /. Anal. Toxicol, 20, 27-37. 

Three different detection modes can be utilized including fixed potential preferred in flow systems and for biosensors, step pulse potential and sweeping potential of which the last two are preferred in batch systems. Up and coming are microchip capillary electrophoresis coupled with amperometric screen-printed electrodes [25], Metal sensors are also used, but lack selectivity. They are more useful for classification applications than for evaluation of taste, like predicting sensorial descriptors of Italian red dry wines of different origins [26]. 

Wiltfang, J. Jean Louis, V. Tavema, M. Analysis of amyloid-P peptides in cerebrospinal fluid samples by capillary electrophoresis coupled with LIE detection. Anal. Chem. 2011, 83,1696-1703. 

Ye H, Yu L, Xu X, Zheng C, Lin W, Liu X, Chen G. Affinity capillary electrophoresis coupling with partial filling technique and field-amplified sample injection for enantiosepara-tion and determination of DL-tetrahydropalmatine. Electrophoresis 2010 31 2049-2054. 

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