Culture of Plant Cells and Tissues

In this review, we focus on the use of plant tissue culture to produce foreign proteins that have direct commercial or medical applications. The development of large-scale plant tissue culture systems for the production of biopharmaceutical proteins requires efficient, high-level expression of stable, biologically active products. To minimize the cost of protein recovery and purification, it is preferable that the expression system releases the product in a form that can be harvested from the culture medium. In addition, the relevant bioprocessing issues associated with bioreactor culture of plant cells and tissues must be addressed. 

Productivity Considerations in Culture of Plant Cells and Tissue... 

Because of cell speciaHzation, some produces are produced in cultures of those cellular types. Three main classifications of the types of plant cell and tissue cultures are ... 

The production of secondaiy metabohtes has often been characterized using the classical equations of Leudeldng and Piret. However, the complexities of plant cell and tissue cultures have led to revisions to this equation to include fresh cell weight and viability, cell expansion, and culture death phase. Therefore, the production model is written as the following ... 

Fujita Y (1988) In Ciba Foundation symposium 137. Applications of plant cell and tissue culture. Wiley, Chichester, p 228... 

Alfermann, A.W. Peterson, M. Plant Cell Tiss. Org, 1995, 43, 199-205. Fujita, Y. In Application of Plant Cell and Tissue Culture, Bock G, Marsh J, Ed. John Wiley Sons UK, 1988 pp. 228-235. 

Fujita, Y. Industrial production of shikonin and berberine. In Applications of Plant Cell and Tissue Culture (Bock, G., Marsh, J., Eds). John Wiley Sons Chichester, 1988, pp. 157—174. 

Because of their economic interest, biotechnological production of these alkaloids would be of great interest particularly as it could contribute to reduce the illicit production of opium, the raw material for heroin manufacture. As a result, extensive studies have been made on the application of plant cell and tissue culture for the production of opium alkaloids. Furthermore bioconversion of certain semisynthetic products has been studied. A series of patents in this field has been filed (Table XXII). 

Y. Yamada and T. Hashimoto, in Applications of Plant Cell and Tissue Culture (Ciba Foundation Symposium 137), p. 199. Wiley, Chichester, 1988. 

Yesil-Celiktas et al. (69) reviewed the literature concerning large-scale cultivation of plant cell and tissue culture in bioreactors, and Ziv (70) reviewed bioreactor technology for the micropropagation of plants. 

Yesil-Celiktas, O., Gurel, A., and Yardar-Sukan, F., Large Scale Cultivation of Plant Cell and Tissue Culture in Bioreactors, p. 3, Transworld Research Network, Kerala, India, 2010. 

Greenhalgh, P. (1982) In Cultivation and Utilisation of Aromatic Plants, (eds Atal, C.K. and Kapur, B.B.). Council of Scientific and Industrial Research, Jammu-Tawi, pp. 139-166. Guenther, E. (1948-52) The Essential Oils. (6 vols), van Nostrand Reinhold, New York. Hall, T.C. and DeRose, R.J. (1988) Transformation of plant cells. In Applications of Plant Cells and Tissue Culture (eds Bock, G. and Marsh, J.) Ciba Foundation Symposium 137, John Wiley, Chichester, pp. 123-143. 

Plant Cells and Tissues Structure-Function Relationships. Methods for the Cytochemical/Histochemical Localization of Plant Cell/Tissue Chemicals. Methods in Light Microscope Radioautography. Some Fluorescence Microscopical Methods for Use with Algal, Fungal, and Plant Cells. Fluorescence Microscopy of Aniline Blue Stained Pistils. A Short Introduction to Immunocytochemistry and a Protocol for Immunovi-sualization of Proteins with Alkaline Phosphatase. The Fixation of Chemical Forms on Nitrocellulose Membranes. Dark-Field Microscopy and Its Application to Pollen Tube Culture. Computer-Assisted Microphotometry. Isolation and Characterization of... 

The purpose of this chapter is to review the state of knowledge about the endophytic fungal species that inhabit the spaces within and between plant cells and tissues and to examine the array of secondary products produced by these fungal inhabitants. In the few cases in which enough is known, the details of the biosynthesis of these compounds, the medicinal uses to which these compounds have, and the potential for culture of the fungi as industrial producers of the metabolites will be discussed. 

Production of anthocyanins in plant cell and tissue cultures has been reported for more than 30 species including Daucus carota, Fragaria x ananassa, Vaccinium spp., Vitis hybrida, Solanum tuberosum, Malus sylvestris, Aralia cordata, Perilla frutescens, Ipomoea batatas. Euphorbia millii, Strobilanthes dyeriana. Hibiscus sabariffa, Dioscorea cirrhosa, etc. ... 

Knorr, D., Caster, C., Dorneburg, H., Dorn, R., Graf, S., Havin-Frenkel, D., Podstolski, A. and Werrman, U. (1 993) Biosynthesis and yield improvement of food ingredients from plant cell and tissue cultures. Food Technology 47, 57-63. 

---