Aniline blue staining

Fluorescence Microscopy of Aniline Blue Stained Pistils... 

The aniline blue stain penetrates macerated tissue very quickly and in a few minutes the slide is ready to be observed. Slides do not dry out for several hours. They can be stored for a longer time (about 2 wk) in a cool, dark place if sealed around the coverslip with parafilm or Vaseline. 

Plant Cells and Tissues Structure-Function Relationships. Methods for the Cytochemical/Histochemical Localization of Plant Cell/Tissue Chemicals. Methods in Light Microscope Radioautography. Some Fluorescence Microscopical Methods for Use with Algal, Fungal, and Plant Cells. Fluorescence Microscopy of Aniline Blue Stained Pistils. A Short Introduction to Immunocytochemistry and a Protocol for Immunovi-sualization of Proteins with Alkaline Phosphatase. The Fixation of Chemical Forms on Nitrocellulose Membranes. Dark-Field Microscopy and Its Application to Pollen Tube Culture. Computer-Assisted Microphotometry. Isolation and Characterization of... 

Decolorize the smear in aniline-xylene, agitating the slide gently until no purple runs from it (the use of a second Coplin jar of aniline-xylene after the majority of blue stain has been removed aids the visual assessment of decolorization). 

Suberized lamellae in casparian strips can be located using the berbe-rine-aniline blue fluorescence staining method, which works equally... 

Brundrett MC, Enstone DE, Peterson CA. A herherine-aniline blue fluorescent staining procedure for suberin, lignin and callose in plant tissues. Protoplasma 1988 146 133-142. 

Protocol for Staining Pistils with Aniline Blue... 

PROTOCOL FOR STAINING PISTILS WITH ANILINE BLUE... 

The medium for maceration (HC1 or NaOH) depends on the material and purpose of staining. If fresh material is at hand, NaOH is best. It keeps material at the proper pH even after washing a few times in water. It is very important to achieve a pH of 9.0-10.0 to obtain the proper fluorescence of callose after staining with aniline blue. 

Solution of aniline blue 0.005% or 0.01% of water-soluble stain in phosphate buffer, pH 9.5. 

Foltz DR, Jansen LE, Black BE, Bailey AO, Yates JR, Cleveland DW (2006) The human CENP-A centromeric nucleosome-associated complex. Nat Cell Biol 8 458 69 Foresta C, Zorzi M, Rossato M, Varotto A (1992) Sperm nuclear instability and staining with aniline blue abnormal persistence of histones in spermatozoa in infertile men. Int J Androl 15 330-337 Fukagawa T, Nogami M, Yoshikawa M, Ikeno M, Okazaki T, Takami Y, Nakayama T, Oshimura M (2004) Dicer is essential for formation of the heterochromatin structure in vertebrate cells. Nat Cell Biol 6 784-791... 

Microscopic counting is still the most direct method of estimating the amount of microbial biomass in soil but is technically difficult and completely unsuitable for routine use. Thin films are prepared from an agar-soil suspension, are mounted on microscope slides, and then are treated with an appropriate stain. Phenolic aniline blue is often used as it stains protein and is thus considered to give an estimate of the entire population. The numbers... 

Figure 8. Histochemical studies of alfalfa callus cells. A. Normal callus cells stained with aniline blue black for total protein before induction of regeneration. B. Callus cells stained with aniline blue black after induction of regeneration very darkly staining material is protein.

Fig. 10. The deposition of callose in the plasmodesmata regions, suggesting the closure of plasmodesmata only between some of the explants cells (A), and in the cell wall, suggesting the isolation of neighbouring cells via apoplast (B) Arabidopsis explants during SE hand-cut sections stained with aniline blue bar = 15 pm).

Fig. 9 Foam-like vesicle structures at the interface stained with aniline blue left) and separated droplets after stirring the foam-like structures (right)...

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