Nanoparticles conjugated

Medley et al. have shown the use of aptamer-conjugated nanoparticles for cancer cell detection [50]. Herr et al. [51] have shown the rapid collection and detection of leukemia cells using a novel two-nanoparticle assay with aptamers as the molecular recognition element. 

MacKay JA, Chen M, McDaniel JR, Liu W, Simnick AJ, Chilkoti A (2009) Self-assembling chimeric polypeptide-doxorubicin conjugate nanoparticles that abolish tumours after a single injection. Nat Mater 8 993-999... 

Fig. 32. (a) Folate-conjugated nanoparticles (b) Fluorescence micrograph of KB cells incubated with the fluorescent probe for 4 h at 37°C. Figure adapted from Ref. (153). 

Hussain, N., Jani, P. U., and Florence, A. T. Enhanced oral uptake of tomato lectin-conjugated nanoparticles in the rat. Pharm. Res. 14 613-618, 1997. 

Hussain N, Jani PU, Florence AT (1997) Enhanced Oral Uptake of Tomato Lectin-Conjugated Nanoparticles in the Rat. Pharm Res 14(5) 613-618 Jackson DC, Lau YF, Le T, Suhrbier A, Deliyannis G, Cheers C, Smith C, Zeng W, Brown LE (2004) A totally synthetic vaccine of generic structure that targets Toll-like receptor 2 on dendritic cells and promotes antibody or cytotoxic T cell responses. Proc Natl Acad Sci U S A 101(43) 15440-15445... 

Solid-Lipid Nanoparticles SLNs have been used to deliver small molecules and macromolecules such as DNA and peptides. The in vitro and in vivo applications of SLNs are reviewed elsewhere [125,126]. The stability and oral bioavailability of insulin were enhanced when administered in wheat germ agglutinin-conjugated nanoparticles [127], A polyoxyethylene stearate coat on the SLN confers stealth properties [128],... 

Na, K., Lee, E. S., and Bae, Y. H. (2003), Adriamycin loaded pullulan acetate/sulfonamide conjugate nanoparticles responding to tumor pH pH-dependent cell interaction, internalization and cytotoxicity in vitro, I. Controlled Release, 87(1-3), 3-13. 

The effect of temperature on fluorescence has been studied, as has the effect of salt concentration and water-soluble conjugated polymers. A method for the quantification of ssDNA dsDNA is described, as well as kinetics of mismatch hybridization and the kinetics of collision in short ss-nucleic acids. Fluorescence quenching of Cy-5 labelled oligonucleotides by poly(phenylene ethynylene) particles has been shown to be a more sensitive method than excitation of the Cy-5 fluorophore. An ultrasensitive method for the detection of DNA uses highly fluorescent conjugated nanoparticles, and detection limits below IfM were achieved. DNA transport through a carbon nanotube has also been observed using fluorescence microscopy. " ... 

Meziani, M. J. and Sun, Y. P. (2003). Protein-conjugated nanoparticles from rapid expansion of supercritical fluid solution into aqueous solution. J. Am. Chem. Soc. 125, 8015-8018. Meziani, M. J., Pathak, P., Hurezeanu, R., Thies, M. C., Enick, R. M., and Sun, Y. P. (2004). Supercritical fluid processing technique for nanoscale polymer particles. Angew. Chem. Int. Ed. Engl. 43, 704—707. 

Herr, J.K., Smith, J.E., Medley, C.D., Shang-guan, D., Tan, W. (2006). Aptamer-conjugated nanoparticles for selective collection and detection of cancer cells. Anal. Chem. 78,2918-2924... 

FIGURE 30.5 Nano- and microparticulate drug carrier systems, (a) Microsphere, (b) microcapsule, (c) surface modified nanoparticles, (d) nanosphere, (e) nanocapsule, and (f) surface modified targeting molecules conjugated nanoparticles. 

Antibody-conjugated Nanoparticles to Enhance the Labeling Efficiency of Islets... 

Polymer linker can be used to conjugate nanoparticles to the surface of islets. One example is a heterofunctional PEG-mediated conjugation method by covalent-linkage [91]. The chemical conjugation was mediated by a heterobifunctional PEG polymer, maleimide-PEG-N-hydroxysuccinimide ester (NHS), which is biocompatible with blood, cell, and tissue. The maleimide group of the PEG polymer can react with the sulfhydryl group of the heparinized superparamagnetic... 

Kim, G.C., et al. Air plasma coupled with antibody-conjugated nanoparticles a new weapon against cancer. Journal of Physics D Applied Physics 42(3), 032005 (2009)... 

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