Agglutinins wheat germ

EC 3.4.24.3]. Purified by using /V-ethylmaleimide to activate the enzyme, and wheat germ agglutinin-agarose affinity chromatography [Callaway et al. Biochemistry 25 4757 1986],... 

Wheat germ agglutinin Widely used in studies of surfaces of normal cells and cancer cells... 

Jansen H., Iwamoto G. and Jackson G. (1998). Central connections of the ovine olfactory bulb formation identified using wheat germ agglutinin-conjugated horseradish peroxidase. Brain Res Bull 45, 27-39. 

Fig. 1. Competition binding screens Bead/ligand compatibility test in early assay development. The beads PVT-WGA, Type A and Type B are based on polyvinyltoluene (PVT) not polyethyleneimine (PEI). PVT, polyvinyltoluene WGA, wheat germ agglutinin Ysi, yttrium silicate.

Figure 10 Proposed model for the cAMP-induced elevation observed in the exocytosis of biotinylated wheat germ agglutinin containing vesicles in pancreatic cells. CPTcAMP = 8-(4-chlorophenylthio)cAMP PKAj = inactive form of protein kinase A PKAa = active form of protein kinase A P = inorganic phosphate bWGA = biotinylated wheat germ agglutinin. (Based on the findings of Ref. 237.)...

Other applications of QCM biosensors include real-time monitoring of the agglutination process of human hepatic normal cells (L-02) and hepatic cancer cells (Bel7402) on QCM electrode in the absence and presence of ConA and wheat germ agglutinin.70 This work demonstrated that the QCM measurement technique based on cell agglutination can be used for discriminating hepatic normal cells from hepatic cancer cells. 

Weissenboeck, A., Bogner, E., Wirth, M., Gabor, F., Binding and uptake of wheat germ agglutinin-decorated PLGA-nanospheres by Caco-2 monolayers. Pharm Res 21, 1919-1925 (2004). 

Gabor, F., Wirth, M., Jurkovich, B., Theyer, G., Walcher, G., Hamilton, G., Lectin-mediated bioadhesion Proteolytic stability and binding-characteristics of wheat germ agglutinin and Solanum tuberosum lectin on Caco-2, HT-29 and human colonocytes. J Control Release 49, 27-37 (1997). 

Wirth, M., Kneuer, C., Lehr, C.M., Gabor, F., Lectin-mediated drug delivery Discrimination between cytoadhesion and cytoinvasion and evidence for lysosomal accumulation of wheat germ agglutinin in the Caco-2 model. J Drug Target 10,... 

Gabor, F., Klausegger, U., Wirth, M., The interaction between wheat germ agglutinin and other plant lectins with prostate cancer cells Du-145. Int J Pharm 221, 35—47 (2001). 

Fiebrig, L, Varum, K.M., Elarding, S.E., Davis, S.S., and Stokke, B.T. Colloidal gold and colloidal gold labelled wheat germ agglutinin as molecular probes for identification in mucin/ chitosan complexes, Carbohydr. Polym., 33 91-99 (1997). 

---