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Complete freunds adjuvant CFA

Materials. Microspherical PGG glucan (Adjuvax, Alpha-Beta Technology, Worcester, MA) was prepared from Saccharomyces cereviseae strain R4 cells (11). Zymosan, cytochrome c (cyt c), bovine serum albumin (BSA), yeast alcohol dehydrogenase (ADH), Complete Freunds Adjuvant (CFA) and Incomplete Freunds Adjuvant (IFA) were purchased from Sigma Chemical Co. (St. Louis, MO). [Pg.55]

This test, performed in rats, is the classical model of inflammatory pain. Intraplantar injection of inflammatory stimuli such as carrageenan, kaolin, or complete Freund adjuvants (CFA) induces paw swelling and increased pain sensitivity. As pain stimulus pressure is applied on the inflammed paw and gradually increased until the animal responds by vocalisation or withdrawal of the paw. Analgesics increase the pressure threshold (Randall and Selitto, Arch. Int. Pharmacodyn. 1957, 111, 409-419). [Pg.591]

The first group underwent a conventional immunization procedure (6) in which one half mg of ECPs were administered subcutaneously in Complete Freunds Adjuvant (CFA) on day one and in Incomplete Freunds Adjuvant (ICFA) on day 7. The rabbits were boosted every 14 days for 160 days and serum collected 7 days after each boost. [Pg.134]

AIA, adjuvant-induced arthritis CFA, complete Freund s adjuvant CIA, collagen-induced arthritis DTH, delayed-type hypersensitivity LPS, lipopolysaccharide. [Pg.174]

Fig. 15 Induction of cellular immunity by subcutaneous immunization with OVA-encapsulating y-PGA-Phe nanoparticles. Mice were subcutaneously immunized one time with OVA alone (10 pg), 10 pg of OVA and 100 pg of NPs (OVA-NPs), 10 pg of OVA and 100 pL of complete Freund s adjuvant (OVA + CFA), or PBS (control). Splenocytes were obtained from the immunized mice on day 10 after the immunization and stimulated with the OVA peptide. The number of IFN-y-producing cells was measured by enzyme-linked immunospot assay. SFU spot forming units... Fig. 15 Induction of cellular immunity by subcutaneous immunization with OVA-encapsulating y-PGA-Phe nanoparticles. Mice were subcutaneously immunized one time with OVA alone (10 pg), 10 pg of OVA and 100 pg of NPs (OVA-NPs), 10 pg of OVA and 100 pL of complete Freund s adjuvant (OVA + CFA), or PBS (control). Splenocytes were obtained from the immunized mice on day 10 after the immunization and stimulated with the OVA peptide. The number of IFN-y-producing cells was measured by enzyme-linked immunospot assay. SFU spot forming units...
PBS = phosphate buffered saline CFA = complete Freund s Adjuvant... [Pg.90]

CEA carcinoembryonic antigen CFA complete Freund s adjuvant CG-motif cytosine-guanine dinucleotide motif... [Pg.197]

Complete Freund s adjuvant (CFA) This is composed of an oil-in-water emulsion containing killed mycobacteria, and exhibits a strong adjuvant effect but also severe toxic side-effects [31]. [Pg.207]

MBSA=methylated bovine serum albumin CFA=complete Freund s adjuvant DH = delayed hypersensititivy Ab=antibody response RB=rabbits GP=guinea pigs H = humans IFA=incomplete Freund s adjuvant... [Pg.23]

Complete Freund s adjuvant (CFA Sigma-Aldrich, F5881)... [Pg.268]

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See also in sourсe #XX -- [ Pg.19 , Pg.702 ]



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