Hybridization colonies

Grunstein, M., and Hogness, D. S., 1975. Colony hybridization A specific method for the isolation of cloned DNAs tliat contain a specific gene. Proceedings of the National Academy of Sciences U.S.A. 72 3961—3965. Article describing die colony hybridization technique for specific gene isolation. 

Palmer, L. M., and Colwell, R. R. (1991). Detection of luciferase gene sequence in nonluminescent Vibrio cholerae by colony hybridization and polymerase chain reaction. Appl. Environ. Microbiol. 57 1286-1293. 

Sayler GS, MS Shields, ET Tedford, A Breen, SW Hooper, KM Sirotkin, W Davis (1985) Application of DNA-DNA colony hybridization to the detection of catabolic genotypes in environmental samples. Appl Environ Microbiol 49 1295-1303. 

DNA adsorption properties were first studied using a variety of solid supports for classical analysis methods including Southern and Northern transfers, dot-blotting, colony hybridization and plaque-lifts [31,32]. Studies of the interactions between nucleic acids and nitrocellulose revealed that molecular weight, finite macromolecular conformation, ionic forces and weaker forces of attraction all play a role. DNA is retained on nitrocellulose only in... 

C.A. Pettigrew and G.S. Sayler. 1986. Application of DNA colony hybridization to the rapid isolation of 4-chlorobiphenyl catabolic phenotypes. J. Microbiol. Methods. 5 205-213. 

Packard, J., Breen, A., Sayler, G.S. Palumbo, A.V. (1989). Monitoring populations of 4-chlorobiphenyl-degrading bacteria in soil and lake water microcosms using colony hybridization. In HMCRl s Second National Conference, Biotreatment - The Use of Microorganisms in the Treatment of Hazardous Materials and Hazardous Wastes, Washington, DC, November 27-29, pp. 119-25. Silver Spring, MD Hazardous Materials, Control Research Institute. 

Pettigrew, C. A. Sayler, G. S. (1986). The use of DNA DNA colony hybridization in the rapid isolation of 4-chlorobiphenyl degradative bacterial phenotypes. Journal of Microbiological Methods, 5, 205-13. 

Limitations notwithstanding, colony hybridization techniques have been used successfully for a number of years to monitor environmental samples. Representative examples include detection of naphthalene and toluene degraders in contaminated soils (Sayler et al., 1985), recombinant organisms in groundwater (Jain et al., 1987 ... 

Large numbers of clones obtained can be screened rapidly by colony hybridization using a labeled DNA probe. Thus, if it is desired to isolate a gene for a particular protein and some part of that protein has... 

The original colony hybridization method described the use of radio-labeled probes and the detection of positive hybridization events by autoradiography (1). However, because of the high waste disposal costs, short half-lives, long autoradiographic exposures, and potential health hazards associated with radioisotopes, there is interest in alternative methods to detect positive hybridizations. 

To facilitate our work on plasmids with no known phenotype, we have developed a method for the use and detection of biotinylated probes in colony hybridization. It is suitable both for the detection of rare positive hybridization events over a background of nonreactive colonies and for the detection of nonhybridizing colonies in a population containing sequences homologous to the probe. The latter capability could be useful in such applications as the detection of cured (i.e., plasmid-free) cells in a bacterial population containing plasmids. 

Wash each filter with 100 mL of chloroform using the Screen-It colony hybridization device A single sheet of filter paper is used as an underfilter... 

Maas, R. (1983) An improved colony hybridization method with significantly increased sensitivity for detection of single genes Plasmid 10, 296-298. 

Christian et al. [69] evaluated the genetic diversity of their dodeca-peptide library by a modified colony hybridization protocol. This protocol allows the discrimination of perfect matches by hybridizing 17 degenerated oligonucleotide probes to the library clones and washing by incrementing the temperature until the perfect matches alone are able to hybridize. The observed number of positive clones for each oligonucleotide probe is correlated with the theorized number. 

Lonvaud-Funel et al. (1989, 1991a) described the identification of LAB during vinification and wine storage by DNA-DNA hybridization. Genomic DNA of the strain to identify was hybridized with total genomic DNA probes extracted from reference strains. They found that this method was particularly efficient when used in colony hybridization to study mixed populations at least five different species can be detected in a mixture with this system (Lonvaud-Funel et al. 1991b). 

Lonvaud-Funel, A., Joyeux, A., Ledoux, O. (1991b). Specific enumeration of lactic add bacteria in fermenting grape must and wine by colony hybridization with non isotopic DNA probes. J. Appl. Bacterial, 71, 501-508. 

Grunstein, M., Hogness, D. (1975) Colony Hybridization A. Method for the Isolation of Cloned DNAs that Contain a Specific Gene, Proc. Natl. Acad. Sci. USA 72,3961-3966. 

Screeiring plasmid-based genomic libraries often uses a technique termed "colony hybridization." This techirique can employ autoradiographic films, which detect ... 

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