Coliform bacteria detection

Escherichia coli detected in 10 ml coliform bacteria detected in 0.1 ml... 

Bej, A. K. McCarty, S. C. Atlas, R. M. Detection of coliform bacteria and Escherichia coli by multiplex polymerase chain reaction comparison with defined substrate and plating methods for water quality monitoring. Appl. Environ. Microbiol. 1991, 57, 2429-2432. 

Industrial microbiological quality control normally covers the total count of germs, the counts of yeasts and moulds, coliform bacteria and E. colt. In special cases these investigations are complemented by the detection of Staphylococcus aureus, salmonella and listeria. 

The standard bacteriological method forjudging the suitability of water for domestic use is the coliform test. It detects the presence of coliform bacteria, which are found in the natural environment (soils and plants) and in the intestines of humans and other warm-blooded animals. Any food or water sample in which this group of bacteria is found is to be suspected of having come into contact with domestic sewage or animal manure. Such a water supply may contain pathogenic bacteria and viruses responsible for typhoid fever, dysentery, and hepatitis (Shelton, 1989). 

Bej A.K., Steffan R.J., DiCesare J., Haff L. and Atlas R.M. (1990) Detection of coliform bacteria in water by polymerase chain reaction and gene probes. Appl. Environ. Microbiol., 56, 307-314. 

In fact, there were warnings of problems. Between January and April of 2000 (the months just prior to the May E. coli outbreak), the lab that tested Walkerton s water repeatedly detected coliform bacteria—an indication that surface water was getting into the water supply. The lab notified the MOE on five separate occasions. The MOE in turn phoned the WPUC, was assured the problems were being fixed, and let it go at that. The MOE did not inform the local Walkerton Medical Office of Health, however, as by law it was required to do. 

Total coliform bacteria Must not be detectable in any 100-mL sample... 

Total coliform bacteria Must not be detectable in any 100-mL sample, hi Hie case of large supplies, where si cient samples are ei mined, must not be present in 95% of samples taken Ihrou out any 12-mon1h period. 

Coliform bacteria should not be detectable in 100 ml of drinking water. In the case of mineral water, they must not be present in 250 ml. 

Coliform bacteria (possibly with details of the type) are detectable in. .. ml. 

Escherichia coli and coliform bacteria are not detectable in 100 ml of water. Membrane-filter method... 

Sheet 3 - Detection of Escherichia coli and coliform bacteria... 

With the titre method, one states the smallest amount of water in which Escherichia coli and coliform bacteria were detected, e.g. 

ONPG and MUG test Water purity test that relies on the ability of coliform bacteria to secrete enzymes that convert a substrate into a product that can be detected by a color change. 

A practical and very useful application of DNA analysis is in detecting coliform bacteria in water supplies. The same techniques can be extended to check for pollution of surface water and beaches by sewage. The importance of this is can easily be understood by looking at the range of pathogenic microorganisms which can be found in fecal material and can from time to time appear in areas... 

International Standard, ISO 9308-1 (2000). Water Quality—Detection and enumeration of Escherichia coli and coliform bacteria—Part 1 Membrane filtration method. International Organization for Standardization, Geneva, Switzerland. 

Toyama, K. Fukuwatari, Y Yano, Y Kiyotaki, K. Nakagawa, M. Kano, K. Sasaki, K. Method and kit for detecting Coliform bacteria. Jpn. Kokai Tokkyo Koho JP 2002360296,2002 Chem. Abstr. 2002,138, 21812. 

A bacteriological examination of water is primarily carried out to determine the possible presence of harmful microorganisms. Testing is actually done to detect relatively harmless bacteria called colon bacilli commonly called the coliform group, which are present in the intestinal tract of humans and animals. If these organisms are present in a water in sufficient number, then this is taken to be evidence that other harmful pathogenic bacteria may also be present. 

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