Chromatography future trends

The objectives of this monograph are to provide a compilation of the newest results in this field of research, a critical evaluation of the results, a comparison of the various LC separation techniques, and the prediction of future trends in the LC analysis of pigments. The book is meant to be self-sufficient in terms of the needs of the average analytical chemist who intends to work in this fascinating field. We are confident that it will be a valuable reference book for researchers and serious students engaged in the chromatography of pigments. 

This chapter presents an overview of current trends in high-pressure liquid chromatography (HPLC) instrumentation focusing on recent advances and features relevant to pharmaceutical analysis. Operating principles of HPLC modules (pump, detectors, autosampler) are discussed with future trends. 

Burnouf T (1995) Chromatography in plasma fractionation benefits and future trends. J Chromatogr B Biomed Appli 664 3-15... 

Some idea of the future trend of methylation analysis can, perhaps, be gained from the work of Perila and Bishop, who have used the method to study oligosaccharides obtained by the enzymic hydrolysis of jack-pine mannan. The oligosaccharides (0.5-2.0 mg.) were methylated by the Kuhn procedure, refluxed with methanolic hydrogen chloride, and the resulting methyl 0-methylglycosides analyzed qualitatively and quantitatively by gas-liquid chromatography. 

For both. A- and B- trichothecenes there is still a lack of simple and reliable screening methods enabling the rapid detection of these mycotoxins at low cost. Besides the increasing demand for rapid screening methods for both A- and B-trichothecens, the use of liquid chromatography with tandem mass spectrometry (LC-MS/MS) enabling both quantification and identification of several trichothecenes simultaneously can be considered a major future trend in the analysis of these Fusarium mycotoxins in cereals (Krska et al., 2001). 

C De Luca, E Quattrucci, S Passi. A gas chromatography-mass spectrometry method for the analysis of tocopherol and low-molecular weight phenol contents in foods of vegetable origin. Current status and future trends. Proceedings of Euro Food Chem VIII, Vienna, 1995, pp 74-78. 

Electrospray in the mid 1980s revolutionized biological mass spectrometry, in particular in the field of protein and peptide sequence analysis. Electrospray is a concentration-dependent, rather than a mass-dependent process, and maximum sensitivity is achieved at low flow rates with high-concentration, low-volume samples (Griffiths 2000). Joint NMR, x-ray diffraction, electrophoresis, and chromatography techniques with mass spectrometry (MS) techniques would be a trend in the future. 

Regnier, F.E., He, B., Lin, S., Busse, J., Chromatography and electrophoresis on chips critical elements of future integrated, microfluidic analytical systems for life science, Trends Biotechnol. 1999, 17. 

Despite the reservations noted above, it would be improper if no comment at all were made on the future of new techniques. In general, the trends of the last few decades suggest that developments in spectroscopy, chromatography and biological methods will continue to take place. Some of these will be in the direction of improving the speed, sensitivity and resolution of existing techniques, others may be the establishment of entirely new methods. 

Regnier FE, He B, Lin S, Busse J (1999) Chromatography and electrophoresis on chips critical elements of future integrated, microfluidic analytical systems for life science. Trends Biotechnol 17 101-106 Cabrera CR, Yager P (2001) Continuous concentration of bacteria in a microfluidic flow cell using electrokinetic techniques. Electrophoresis 22(2) 355-362 5. Xuan X, Li D (2005) Focused electrophoretic motion and selected electrokinetic dispensing of particles and cells in cross-microchannels. Electrophoresis 26(18) 3552-3560... 

The trend has been toward the increased nse of GC in the petrolenm industry. This is due to the relatively low cost of a gas chromatographic system that can provide more detailed analyses as well as the detection of trace components. Gas chromatography has also provided a means for online process monitoring. Future utilization of GC will involve the establishment of basic relationships between composition and performance parameters. This will allow the substitution of this rapid and reliable chromatographic analysis for the empirical methods from the past. 

Trends. The results of a survey of 12 leading experts in gas chromatography appeared in 1989 and outlined their thoughts on projected trends in gas chromatographic column technology, including the future of packed columns versus capillary columns (11). Some responses of that panel are ... 

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