Sequence analysis, peptide

Whilst chymotrypsin and trypsin are especially useful in peptide sequence analysis, they also have medicinal applications. Their ability to hydrolyse proteins makes them valuable for wound and ulcer cleansing (trypsin) or during cataract removal (chymotrypsin). 

Martin, S. E., Shabanowitz, J., Hunt, D. R, and Marto, J. A., Subfemtomole MS and MS/MS peptide sequence analysis using nano-HPLC micro-ESI Eourier transform ion cyclotron resonance mass spectrometry, Analytical Chemistry 72(18), 4266 274, 2000. 

Electrospray in the mid 1980s revolutionized biological mass spectrometry, in particular in the field of protein and peptide sequence analysis. Electrospray is a concentration-dependent, rather than a mass-dependent process, and maximum sensitivity is achieved at low flow rates with high-concentration, low-volume samples (Griffiths 2000). Joint NMR, x-ray diffraction, electrophoresis, and chromatography techniques with mass spectrometry (MS) techniques would be a trend in the future. 

Bhown, A. S. (1988) Protein/Peptide Sequence Analysis Current Methodoligies, CRC Press, Boca Raton, Florida... 

Rangarajan, M. (1988)./n Protein/Peptide Sequence Analysis Current Methodologies , A.S. Bhown, ed. 135-144. 

High Sensitivity Peptide Sequence Analysis Using In Situ Proteolysis on High Retention PVDF Membranes and a Biphasic Reaction Column Sequencer... 

C. Evaluation of the HP Sequencer for High Sensitivity Peptide Sequence Analysis... 

Peptide sequence analysis (PSA) of the peptides that contains a PTM. If the amino-acid sequence of the protein is not known or the position is not unambiguously determined in PMF, sequence analysis of the modified peptides is necessary. Strategies for shotgun identification (Ch. 18.3.2 ) of posttranslation modifications are reviewed by Cantin and Yates [4]. 

Capillary isoelectric focusing coupled to mass spectrometry has gained popularity in recent years (by analogy to two-dimensional gel electrophoresis). Additional information obtained from mass spectrometry includes not only precise molecular-weight determination but also the possiblity for peptide sequencing. Analysis of hemoglobin variants, recombinant proteins, and monoclonal antibodies have been demonstrated [1,7,8]. 

THF. 2 28.ii7,ii8 Pq]. instance, the thioxobenzoylcholine (52), which is a powerful A(-thiobenzoylating agent itself and can be advantageously used in peptide sequence analysis, was prepared according to equation (49). ... 

MW Hunkapillar. In AS Brown, ed. Protein/Peptide Sequence Analysis Current Methodologies. Boca Raton, FL CRC Press, 1988, pp 87-117. 

Spectrometry Strategies for Peptide Sequence Analysis and Characterization.87... 

Martin, S.E., Shabanowitz, J Hunt, D.F, Marto, J.A. (2000) Subfemtomole MS and MS/MS Peptide Sequence Analysis Using Nano-HPLC Micro-ESI Fourier Transform Ion Cyclotron Resonance Mass Spectrometry. Anal. Chem. 72 4266-4274. 

Morris HR, Panico M, Barber M, Bordoli RS, Sedgwick RD, Tyler A. Fast atom bombardment a new mass spectrometric method for peptide sequence analysis. Biochem Biophys Res Commiin. 1981 101 623-31. 

Trimpin, S. Deinzer, M. L. Solvent-free mass spectrometry for hydrophobic peptide sequence analysis and protein conformation studies. Biotechniques 2005,39, 799-805. 

A. N. Fast Atom Bombardment a New Mass Spectrometric Method for Peptide Sequence Analysis. Biochem. Biophys. Res. Commun. 1981,707,623-631. 

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