Chromatography computerized

Gas-liquid chromatography is a qualitative, but also quantitative, method of alkaloid analysis. It is very sensitive. The only problem concerns the distribution of the alkaloid mixture in the chromatographic process and the identification of alkaloids, which must be achieved by a different technique . A very positive characteristic is the possibility of totally computerizing this method of alkaloid detection. 

One determines the overall reaction by using some of the many tools of chemical analysis. Coulometeiy, gas chromatography, mass spectroscopy, and, in complex cases, computerized pattern-recognition programs applied to GC-MS data (see Section 7.5.19) are the typical tools used in determining the overall reaction. The current efficiency for the reaction concerned is important here and may vaty with potential. 

Alme B, Bremmelgaard A, Siovall J, Thomassen P (1977) Analysis of metabolic profiles of bile acids in urine using a lipophilic anion exchanger and computerized gas-liquid chromatography-mass spectrometry. J Lipid Res 18 339-362... 

Back P, Siovall J, Siovall (1974) Monohydroxy bile acids in plasma in intrahepatic cholestasis of pregnancy. Identification by computerized gas chromatography-mass spectrometry. Med Biol 52 31-38... 

MG Scanlon, HD Sapirstein, W Bushuk. Computerized wheat varietal identification by high-performance liquid chromatography. Cereal Chem 66 439-443, 1989. 

TB Whitaker, JW Dickens, AB Slate. Computerized system to quantify aflatoxin using thin-layer chromatography. Peanut Sci 17 96-100, 1990. 

Compound identifications were made by combined gas chromatography-mass spectrometry (GC-MS) based on relative retention times and mass spectral interpretations. The instrument used was a Finnigan 5100 computerized GC-MS system equipped with a 50 m x 0.32 mm i.d. fused silica capillary coated with CP Sil 8 CB (0.25 jim film thickness). Helium was used as carrier gas and the temperature program was as follows 110°C (2 min)- 3°C/min - 320°C. 

R. M. Smith, Forensic identification of opium by computerized gas chromatography/mass spectrometry, J. Forens. Sci., 18 321 (1973). 

Application of High-Speed, Integrated, Computerized, Hydrodynamic Chromatography for Monitoring Particle Growth During Latex Polymerization... 

In the earlier publications (1,2) it was shown how hydrodynamic chromatography (HDC) could be applied in the study of polymer latexes to determine particle-size. An improved technique for the HDC was developed which utilized higher efficiency and resolving power columns to significantly reduce the analysis time (3). A high speed integrated computer was included in this improvement so that both particle-size and particle-size distribution of latexes could be quantified in the relatively short period of time. This high speed computerized version of the HDC has been used extensively for measurements on the final latex. 

Pereira WE, Hughes BA. 1980. Determination of selected volatile organic priority pollutants in water by computerized gas chromatography-quadrupole mass spectrometry. J Am Water Works Assoc 72 220-230. 

H. H. Maurer, Identification and differentiation of barbiturates, other sedative-hypnotics and their metabolites in urine integrated in a general screening procedure using computerized gas chromatography-mass spectrometry, J. Chromatogr., 530 301 (1990). 

I. Mohiar, Computerized design of separation strategies by reversed-phase liquid chromatography Development of DryLab software, J. Chromatogr. A 965 (2002), 175-194. 

D. W. Hill,T. R. Kelley, and K. J. Langner, Computerized library search routine for comparing ultraviolet spectra of drugs separated by high-performance liquid chromatography, Anal. Chem. 59 (1987), 350-353. 

There is hardly any laboratory without at least a computerized instrument. A good example is the Chromatography Data System (CDS). Before, these instruments create graphs on a paper, and we cut them out and weigh them to determine the component. Today every chromatograph is connected to a CDS, which makes all the calculations according to how we set it up. 

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