DryLab® software

I. Mohiar, Computerized design of separation strategies by reversed-phase liquid chromatography Development of DryLab software, J. Chromatogr. A 965 (2002), 175-194. 

Figure 10-1. DryLab software version 3.0 modeling the separation of a mixture of naphthalenes. Resolution of the critical pair (the two peaks that elute closest together) is denoted as a function of time of gradient. Experimental runs are shown as sohd lines on the resolution map selected prediction is a dashed line.

A drug substance method for a neutral molecule illustrating the use of DryLab software for mobile phase and column optimization... 

Snyder, L.R. and Wrisley, L. (2006) Computer-Facilitated HPLC Method Development Using DryLab Software, in HPLC Made to Measure, (Ed. S.Kromidas), Wiley-VCH, Weinheim. 

Figure 17 Resolution map for the separation of rhGH tryptic digest. Conditions 15 X 0.46-cm Nucleosil Cis column gradient of 0-60% B solvent A is 0.1% TFA in water, solvent B is 0.08% TFA in acetonitrile 1.0 mL/min 40 C. (a) Separation for 120-min gradient (b) resolution map as a function of gradient time (see text for details), DryLab software. (From Ref. 48.)...

DryLab software is just one of the computer simulation software packages available for optimization and method development for GC. The initial version of DryLab... 

In the GC mode, two initial temperature-programmed runs conducted over the same temperature range (i.e., the same initial and final temperatures) at two different heating rates are required for initial input data. For the best combination of predictive range and accuracy, these rates should differ from one another by a factor of about 3. A measured value for and an average value of the column plate number must be entered as well. DryLab software then uses these input data to calibrate the model and consequently, simulating separations of other conditions. 

Computer-Facilitated HPLC Method Development Using DryLab Software... 

DryLab software can be efiectively used for preparative or semi-preparative H P LC method development and optimization. In this example, the laboratory was asked to provide at least 1 g of a chromatographicaUy purified steroid, with a three day turnaround time. The desired compoimd only differs from the major impurity in the spatial orientation of a specific hydroxyl group, and synthetic chemists had been imable to synthesize the pure steroid in a purity of greater than 88%. 

According to Molnar and Rieger, the necessary simulation and calculation can be carried out with DryLab software by means of Excel spreadsheets as shown in Figs. 13 and 14. an evaluation enlarged fundamentally is given with now available Peak Match software. The results of the calculation of robustness of the critical resolution with respect to gradient run time and pH of the aqueous mobile phase A are illustrated in Fig. 13. 

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