Chitosan microparticles

Chitosan microparticles were prepared with tripolyphosphate by ionic cross-hnking, starting from chitosan acetate 1% and oil as an emulsion in the presence of the surfactant Tween-80 2% the o/w 1 10 emulsion was introduced into tripolyphosphate solution by a spray gun. The microparticles were then washed their sizes were in the 500-710 jim range. As the pH of tripolyphosphate solution decreased and the molecular weight of chitosan increased, the microparticles had a more spherical shape and smoother surface [98]. 

Van der Lubben, I.M., et al. 2001. Chitosan microparticles for oral vaccination preparation, characterization and preliminary in vivo uptake studies in murine Peyer s patches. Biomaterials 22 687. 

H. E. Junginger. 2001. In vivo uptake of chitosan microparticles by murine Peyer s patches visualization studies using confocal laser scanning microscopy and immunohistochemistry. 

Krauland et al. [93] prepared the microparticles with thiolated chitosan (chitosan-TBA chitosan-4-thiobutylamidine conjugate) intended for nasal peptide delivery. During the preparation process microparticles were stabilized by the formation of inter- and intramolecular cross-linking via disulfide bonds. Chitosan-TBA microparticles were characterized by improved swelling ability and displayed 3.5-fold higher insulin bioavailability compared to unmodified chitosan microparticles. 

Microparticles Spray drying BSA Starch, alginate, chitosan, carbopol In vitro Chitosan microparticles provided most desirable characteristics for protein delivery 54... 

Van der Lubben, I. M., Kersten, G., Fretz, M. M., Beuvery, C., Verhoef, J. C., and Junginger, H. E. (2003), Chitosan microparticles for mucosal vaccination against diphteria Oral and nasal efficacy studies in mice, Vaccine, 21,1400-1408. 

Krauland, A. H., Guggi, D., and Bernkop-Schnurch, A. (2006), Thiolated chitosan microparticles A vehicle for nasal peptide drug delivery, Int. J. Pharm., 307, 270-227. 

K. Yoshizuka, Z. Lou, and K. Inoue, "Silver-complexed chitosan microparticles for pesticide removal", React. Fund. Polym., Vol. 44, pp. 47-54, 2000. 

Guliyeva U, Oner F et al (2006) Chitosan microparticles containing plasmid DNA as potential oral gene delivery system. Eur J Pharm Biopharm 62 17-25... 

Casettari, L., Castagnino, E., Stolnik, S., Lewis, A., Howdle, S.M., and Ilium, L. Surface characterisation of bioadhesive PLGA/Chitosan microparticles produced by supercritical fluid technology. Pharmaceutical Research 28 (2011) 1668-1682. 

Wisuitiprot W, Somsiri A, Ingkaninan K, Waranuch N. A novel technique for chitosan microparticle preparation using a water/silicone emulsion Green tea model. Int J Cosmet Sci. 2011 33(4) 351—358. 

Li et al. [44] reported that alginate coated chitosan microparticles could be an effective means for mucosal immunization and oral intake of antigens. Mourya and Inamdar [50] prepared chitosan capsules encapsulating 5-aminosalicylic acid for colon-specific delivery. 

Chu, B. Y., Kobaisi, M. A., Zeng, W., Mainwaring, D., Jackson, D. C. (2011). Chitosan microparticles and nanoparticles as biocompatible delivery vehicles for peptide and protein-based immunocontraceptive vaccines, Mol. Pharm.. 9(1), 81-90. 

Gomaa, Y. A., El-Khordagui, L. K., Boraei, N. A., Darwish, 1. A. (2010). Chitosan microparticles incorporating a hydrophilic sunscreen agent, Cgrbgl drPgl, 81, 234-242. 

Wisuitiprot, W, Somsiri, A., Ingkaninan, K., Waranuch, N. (2011). In vitro human skin permeation and cutaneous metabolism of catechins from green tea extract and green tea extract-loaded chitosan microparticles, 33(6), 572-579. 

Several studies in animal models have been carried out to prepare chitosan microparticles-based vaccines against influenza, pertussis, and diphtheria. The nasal chitosan vaccine delivery system has been tested for vaccination against influenza in humans [56]. The results showed that it was both effective and protective according to the Committee for Proprietary Medical Products (CPMP) requirements. 

Transport of chitosan microparticles for mucosal vaccine delivery in a human intestinal M-ceU model. Research van der Lubben et al. (2002)... 

Li et al. (2008). The prepared alginate-coated chitosan microparticles, with mean diameter of about 1 pm, were evaluated for mucosal vaccine. Moreover, alginate coating as carrier could modulate the release behavior of BSA from alginate-coated chitosan microparticles and could effectively protect model protein (BSA) from degradation in acidic medium in vitro for at least 2h. 

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