Plant cell diagnostics

The book is divided into 3 sections. Section I.Cellular Model Systems, includes 3 chapters (Allelopathy and plant cell diagnostics, Cellular models as biosensors and microalgae for determining the effects of allelochemicals). Section II. New Methods of Microscopy has 5 chapters (Microscopic methods to study morpho-cytological events during the seed germination,... 

Hellwig S, Drossard ), Twyman RM, Fischer R (2004) Plant cell cultures for the production of recombinant diagnostic and therapeutic proteins. Nature Biotechnol 22 1415-1422. 

The visible fluorescence of intact living cells is called autofluorescence. If the cells treated with special fluorescent dyes, the cellular common fluorescence also includes the additional light emission. Autofluorescence could be used (i) in express-microanalysis of the accumulation of the secondary metabolites in secretory cells without long biochemical procedures (ii) in diagnostics of cellular damage and (iii) in analysis of cellcell interactions (Roshchina, 2003). During the plant development, secretory... 

Horseradish peroxidase (HRP) is an extracellular plant enzyme that acts in regulation of cell growth and differentiation, polymerization of cell wall components, and the oxidation of secondary metabolites essential for important pathogenic defense reactions. Because of these essential functions, and also because of its stability and ready availability, HRP has attracted considerable attention.13 It has been involved in a number of applications, such as diagnostic assays,14 biosensors,15 bioremediation,16 polymer synthesis,17 and other biotechnological processes.18 More applications in which HRP catalysis is translated into an electrochemical signal are likely to be developed in the near future. 

The multilocus method discovered by Alec Jeffreys has proved useful in a wide range of animal and plant species and is currently one of the most widely used fingerprinting methods in population studies. This method, using probes 33.6 and 33.15, is the only fingerprinting method for which a formal international quality control system is operated (Zeneca Cellmark Diagnostics), and offers a unique opportunity for the standardization of cell line quahty control procedures in widely distributed laboratories. 

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