Chimeric receptors

Retinoic acid All trans-retinoic acid ATRA induces remission of Chimeric receptor (PML-RARa) blocks... 

Wurch T, Colpaert FC, Pauwels PJ. Chimeric receptor analysis of the ketanserin binding site in the human 5-hydroxytryptaminelD receptor importance of the second extracellular loop and fifth transmembrane domain in antagonist binding. Mol Pharmacol 1998 54 1088-1096. 

Nishiya, T., DeFranco, A.L. Ligand-regulated chimeric receptor approach reveals distinctive sub-cellular localisation and signaling properties of the Toll-like receptors. J Biol Chem 279 (2004) 19008-19017. 

The above studies were conducted on chimeric receptors put together from the sequences of two receptors. Subsequent studies were directed at biopolymer sets containing larger variations. One of these studies utilized a set of chimeric and point-mutated oq-adrenoceptors (a,-Alls) that allowed a much richer description compared to the binary descriptions used above. The biopolymer set in the study comprised in total 18 a,-Alls, namely the wild-type ala, alb, and ald-ARs and 15 mutated oq-ARs. The latter had been constructed by exchanging TM segments of ala-AR with the corresponding segments of the alb or ald-ARs (i.e., cassette... 

Aasland, D., Schuster, B., Grotzinger, J., Rosejohn, S., and Kallen, K. J. (2003). Analysis of the leukemia inhibitory factor receptor functional domains by chimeric receptors and cytokines. Biochemistry 42, 5244-5252. 

Verrall S, Ishii M, Oien M, Wang L, Tram T, Coughlin SR The thrombin receptor second cytoplasmic loop confers coupling to G -like G proteins in chimeric receptors. J Biol Chem 272 6898,1997... 

The cited reviews pertaining to GABA/ben-zodiazepine receptors discuss numerous other studies that employ chimeric receptors and site-directed mutagenesis to determine the key structural components of GABA receptor subtypes comprised of various a, j8, and 7 subunit permutations. 

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