Chimerism analysis

Weit This means that from the chimeric analysis one imagines that what you are beginning to see are genetic contributions to the tissue-level organization of the oscillator. 

Tikhonova, E.B., Wang, Q., and Zgurskaya, H.L (2002) Chimeric analysis of the multicomponent multidrug efflux transporters from Gram-negative bacteria. Journal of Bacteriology, 184 (23), 6499-6507. 

Elkins, C.A. and Nikaido. H. (2003) Chimeric analysis of AcrA function reveals the importance of its C-terminal domain in its interaction with the AcrB multidrug efflux pump. Journal of Bacteriologf, 185 (18), 5349-5356. 

Ciruna, B.G., Schwartz, L., Harpal, K., Yamaguchi, T.P., Rossant, J. 1997. Chimeric analysis of fibroblast growth factor receptor-1 (Fgfrl) function a role for FGFR1 in morphogenetic movement through the primitive streak. Development 124, 2829-2841. 

Knockdown ES cells can also be used for chimeric analysis (92). For such experiments, the ES cells can be combined with diploid embryos and their ability to contribute to particular lineages can be investigated. As with aU chimera studies, either the ES cell or the host embryos (or both) must carry a ubiquitous genetic marker just as EGFP or lacZ (84). 

Kimura N, A Nishi, M Goto, K Furukawa (1997) Functional analysis of a variety of chimeric dioxygenases constructed from two biphenyl dioxygenases that are similar structurally but different functionally. J Bacterial 179 3936-3943. 

The structural analysis of the 40s loop is more complex. There are only minor differences at residues 38 and 43-45, but from residue 39 to residue 42 the FKBP13 backbone is displaced by up to 2 A from that of FKBP12. How can the extensive modifications of the chimeric FKBP result in a... 

Koonin, E. V., Mushegian, A. R., Galperin, M. Y., and Walker, D. R. (1997). Comparison of archaeal and bacterial genomes computer analysis of protein sequences predicts novel functions and suggests a chimeric origin for the archaea. Mol. Microbiol. 25, 619-637. 

Foran, J., Norton, A.J., Micallef, L, Taussig, D., Amess, J., Rohatiner, A., and Lister, T.A., Loss of CD20 expression following treatment with Rituximab (chimeric monoclonal anti-CD20) a retrospective cohort analysis, Br.. Haematol., 114, 881-883, 2001. 

Schmidt HH, Strehl S, Thaler D, Strunk D, Sill H, Linkesch W, Jager U, Sperr W, Greinix HT, Konig M, Emberger W, Haas O A (2004) RT-PCR and FISH analysis of acute myeloid leukemia with t(8 16)(p 1 l p 13) and chimeric MOZ and CBP transcripts breakpoint cluster region and clinical implications. Leukemia 18 1115—1121... 

The immunoconjugate of doxorubicin with a chimeric anti-Le -related, tumour-associated antigen expressed on most human carcinomas, was evaluated in phase I [132] and phase II [133] clinical trials. The phase II trial performed in patients with metastatic breast carcinoma showed low clinical response rates. These data together with tumour biopsy analysis suggest that the dose that could be safely administered was insufficient to maintain the intra-tu-... 

Palmer TM, Benovic JL, Stiles GL (1996) Molecular basis for subtype-specific desensitization of inhibitory adenosine receptors. Analysis of a chimeric A Aj adenosine receptor. J Biol Chem 271(25) 15272-15278... 

In addition to the C-terminus, several other regions of Ga have been implicated in receptor binding by a variety of experimental approaches (Fig. 4). Residues in the a4//16 loop contribute to the binding surface as demonstrated by alanine-scanning mutagenesis (Onrust et al, 1997), studies with chimeric Ga proteins (Bae et al., 1997, 1999), sequence analysis of conserved amino acids in Ga subclasses (Lichtarge et al., 1996), chemical cross-linking (Cai et al, 2001), and protection from tryptic proteolysis (Mazzoni and Hamm, 1996). The C-terminal 11 amino acids of Gat and... 

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