Cells visualizing

Living cells visualization of membranes, lipids, proteins, DNA, RNA, surface antigens, surface glycoconjugates membrane dynamics membrane permeability membrane potential intracellular pH cytoplasmic calcium, sodium, chloride, proton concentration redox state enzyme activities cell-cell and cell-virus interactions membrane fusion endocytosis viability, cell cycle cytotoxic activity... 

Sorkin, A., M. McClure, F. Huang, and R. Carter. Interaction of EGF receptor and grb2 in living cells visualized by fluorescence resonance energy transfer (FRET) microscopy. CurrBiol. 10 1395-8.2000. 

Medalia O, Weber I, Frangakis AS, Nicastro D, Gerisch G, Baumeister W (2002) Macro-molecular architecture in eukaryotic cells visualized by cryoelectron tomography. Science 298 1209-1213... 

A. Microscopic and optical arrangement with components for fluorescence excitation and cell visualization, micromanipulatory assembly. All components above the microscope base plate are seen. 

The transmembrane protein VSVG is a classic tool used to study protein transport through the secretory pathway. Some early studies of vesicular transport in living cells visualized the movement of a GFP-tagged version of VSVG by time-lapse confocal microscopy (21, 22). From these data, it was possible to derive a kinetic model of the movement of this well-studied protein between intracellular compartments, while at the same time providing information about the nature of the vesicular structures involved in this process (21, 22). 

Farina, K. L., Wyckoff, J. B., Rivera, J., Lee, H., Segall, J. E., CondeeUs, J. S. and Jones, J. G. (1998). CeU motility of tumor cells visualized in Uving intact primary tumors using green fluorescent protein. Cancer Res. 58, 2528-2532. 

We described details of procedures for real-time imaging of adoptively transferred B cells visualized with fluorescent dyes in the inguinal lymph node. This method can also be used to observe behavior of other immune cells (T lymphocytes, dendritic and follicular dendritic cells, etc.) under a variety of experimental conditions. 

The activity was determined by reduction of viral cytopathic effect and toxicity by examination of cells visually and their ability to grow at normal rate immediately after adding drug-free medium. The ratio of minimum toxic dose and active dose was 100 for both compounds. Five related compounds had a ratio of 32, and 19 other assorted purines and pyrimidines were inactive [220]. The A -allyl-A -ethylpyrimidine (XLVIII) is the best of a small series in which the substituents at the 3, S and 6 positions were varied. Activity was found against herpes virus in vitro and in rabbits eyes [221]. The compound has been evaluated in man and a 1 per cent solution was said to be effective against all recurring herpetic skin diseases [222]. 

Hashimoto, K., Kataoka, N., Nakamura, E., Hagihara, K., Okamoto, T., Kanouchi, H., et al. (2012). Live-cell visualization of the trans-ceUular mode of monocyte transmigration across the vascular endothelium, and its relationship with endothehal PECAM-1. The Journal of Physiological Sciences, 62(1), 63-69. 

Manipulation of fish germ cell visualization, cryopreservation and transplantation. J Reprod Dev, 52, 685-693. 

Das et al. reported a nanoformulation with a tripolymeric composite carrier (alginate, chitosan and Pluronic) by ionotropic pre-gelation followed by polycationic crosslinking for the deUvery of curcumin to cancer cells. The IC50 values for fi"ee curcumin and encapsulated curcumin on Hela cells were found to be 13.28 and 14.34 pM, respectively. The green fluorescence inside the Hela cells visualized using fluorescent microscopy confirmed the cellular internalization of curcumin-loaded composite NPs [ 133]. A simple nanoprecipitation method resulted... 

Table 1 Immunophenotype of lymphocyte subsets and stem cells, visualized by RTC or PE readyconjugated mAbs and analysed by FACS...

Figure 44.15 Live cell uptake of complex 24 in PC3 cells, visualized by time-lapse confocal microscopy imaging [93].

This technique may appear to be related to pressure drop measmements, since in many investigations the two techniques have been carried out for characterization of the two-phase flow. As can be imagined, investigation is limited to single cells. Visualization of flow in fuel cells can be carried out in two types of cells ... 

Figure 2.4 Schematic of solvent-solvent, solvent-polymer and polymer-polymer arrangements in a lattice of N cells visual illustration of combinatorial entropy...

Rothen-Rutishauser, B.M., Schiirch, S., Haenni, B., Kapp, N. and Gehr, P. (2006) Interaction of fine partides and nanopartides with red blood cells visualized with advanced microscopic techniques. Environmental Science Technology, 40,4353-9. 

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