Cells, surface plasmon resonance

Both heparin and heparan sulfate interact with CS protein and help in the binding of the parasite to liver cells. Surface plasmon resonance (SPR) experiments indicate that heparin has fast on-rate k and slow off-rate (kd) kinetics for CS protein with a of 41 nM. Isothermal titration calorimetry shows that the minimum sequence within the heparin polymer capable of a strong interaction with the CS protein is a decasaccharide. High concentrations of exogenous heparin or heparan sulfate can inhibit the binding of CS protein to HepG2 cell, a human hepatoma cell line. However, at low concentrations, heparin or heparan sulfate can also act as a... 

Spectroscopic ellipsometry, flow cell ATR-FTIR, flow cell Quartz crystal microbalance, flow cell Surface plasmon resonance, flow cell Surface force microscopy... 

Finally, a recent paper by Gunther et aL shows that SEK uniquely binds to the T-cell receptor (human V/35.1) through a 15 amino-acid loop (q 3-/38) not evident in other superantigens, except for those in the same toxin grouping (e.g., SET). Surface plasmon resonance studies reveal a 6 (tmol binding affinity of SEK for V/35.1, which fits the range for other SEs when used in this technique. 

Kim, K.-P., Jagadeesan, B., Burkholder, K. M., Jaradat, Z. W., Wampler, J. L., Lathrop, A. A., Morgan, M. T., and Bhunia, A. K. (2006a). Adhesion characteristics of Listeria adhesion protein (LAP)-expressing Escherichia coli to Caco-2 cells and of recombinant LAP to eukaryotic receptor Flsp60 as examined in a surface plasmon resonance sensor. FEMS Microbiol. Lett. 256,324-332. 

Leonard, P., Hearty, S., Quinn, J., and O Kennedy, R. (2004). A generic approach for the detection of whole Listeria monocytogenes cells in contaminated samples using surface plasmon resonance. Biosens. Bioelectron. 19,1331-1335. 

Kyo, M., Yamamoto, T., Motohashi, H., Kamiya, T., Kuroita, T., Tanaka, T., Engel, J. D., Kawakami, B. and Yamamoto, M. (2004) Evaluation of MafG interaction with Maf recognition element arrays by surface plasmon resonance imaging technique. Genes Cells 9, 153-164. 

S. Boussasad, and N. J. Tao, High-Performance Differential Surface Plasmon Resonance Sensor Using Quadrant Cell Photodetector Rev. Sci. Instrum. 2003, 74, 150 M. Suzuki, F. Ozawa, W. Sugimoto, and S. Aso, Miniature Surface-Plasmon Resonance Immunosensors—Rapid and Repetitive Procedure, Anal. Bioanal. Chem. 2002,372, 301. 

Electro-optic effects induced by doping liquid crystals with one-dimensional metal nanoparticles were not only investigated in standard electro-optic test cells, but also in costume-made cells consisting of a thin layer of liquid crystal either deposited onto a thin film of alumina with embedded GNRs [443], or using rubbed polyimide alignment layers modified with solution-cast GNR [444]. In both cases, surface plasmon resonance frequencies of the GNR integrated into these liquid crystal cells could be electrically controlled. 

Pharmacokinetic data analysis requires determination of the analyte in various body fluids. In the case of therapeutic antibodies, serum is the most common matrix to be analyzed. For a critical interpretation of pharmacokinetic data the chosen bioanalytical methods must be considered. The most frequently used for mAbs include enzyme-linked immunosorbent assay (ELISA), capillary electrophoresis (CE)/polyacrylamide gel electrophoresis (PAGE), fluorescence-activated cell sorting (FACS), and surface plasmon resonance (SPR). The challenges and limitations of bioanalytical methods used for the analysis of mAb concentrations are discussed in detail in Chapter 6. 

Plant AL, Brigham-Burke M, Petrella EC, Oshannessy DJ (1995) Phospholipid/alkanethiol bilayers for cell-surface receptor studies by surface plasmon resonance. Anal Biochem 226 342-348... 

The instrument based upon surface plasmon resonance detection (5,9-111 is now routinely used to measure the binding of an antigen (or antibody) to an immobilized antibody (or antigen) in a flow cell. The technology relies on the covalent immobilization of one of the interacting species and the detection of the adsorbed analyte. The sophistication of this expensive instrumentation makes its use difficult for routine investigations in many laboratories. Furthermore, the models used to extract the rate constants are not always appropriate to the kinetic data analysis [ 12-141. 

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