Constant Monod

Table 3.1 shows the kinetic parameters for cell growth, rate models with or without inhibition and mass transfer coefficient calculation at various acetate concentrations in the culture media. The Monod constant value, KM, in the liquid phase depends on some parameters such as temperature, initial concentration of the carbon source, presence of trace metals, vitamin B solution, light intensity and agitation speeds. The initial acetate concentrations in the liquid phase reflected the value of the Monod constants, Kp and Kp. The average value for maximum specific growth rate (/xm) was 0.01 h. The value... 

A literature value for the Monod constant for a Saccharomyces cerevisiae fermentation is. S ,s = 25mg/liter. How does this affect the simulation in Example 12.7 ... 

The maximum specific growth rate pm, Monod constant Km, and cell yield coefficient Y are constants. In (E4-33), C2o is the inlet glucose concentration. 

At incipient washout, the critical dilution rate, DCIll, is related to the Monod constants by >crit = hm5 o/(Xs + So) (equation 5.148)... 

For limiting nutrients, cellular concentrations are constant under conditions of steady-state growth. To ensure that the limiting nutrient is not diluted in the microbial population, kmt must be greater than the maximal growth rate, /imax. This limiting condition sets a minimum for the value of the Monod constant, Kmd = / max /[7]- Note that while Monod kinetics are more applicable than first-order kinetics for many ecological uptake processes, solutions of the above equations require considerably more a priori information [48]. 

How to Find the Monod Constants from the Batch Experiment Method (a). Rearrange Eq. 3 to give... 

Figure 29.4 Evaluating the Monod constants from mixed flow reactor data.

KiM is the Monod constant (mol L-1) equivalent to the chemical concentration of i at which population growth is half maximal... 

The behaviour of the cell productivity DX with varying dilution rate and Monod constant Ks is shown in Fig. 5.58. It may be seen that it becomes zero when the dilution rate is D n and also that it passes through a maximum when plotted against... 

The domination of microbial growth kinetics by the Monod equation has led to the development of techniques to determine the constants Ks and fim used in that equation. Whilst n is in fact dependent on other parameters, such as temperature and pH, in the usual case these are specified, and a design procedure requires values of the Monod constants under these conditions. The yield coefficient Y will also be required in order to link calculations of microbial growth to substrate concentrations. 

The steady-stale substrate and biomass concentrations for a continuous stirred-tank fermenter operated at various dilution rates are given below. Given that the fresh feed concentration is 700 mg/l, calculate the values of the Monod constants and Ks the yield coefficient, Y and the endogenous respiration coefficient kd. 

So is the constant concentration of growth substrate that is added at flow rate D to the chemostat. S and B are the concentrations of growth substrate and bacteria in the chemostat, respectively. K is the Monod constant, which is formally identical to the Michaelis constant of enzyme kinetics, p is the maximal growth rate constant, and Y is the amount of bacterial cells produced from a given amount of substrate (yield). 

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