Cell proliferation quantification

Additionally, two studies have measured colorectal epithelial cell proliferation and apoptosis in human non-neoplastic mucosa in combination with serum bile acid quantification. Ochsenkuhn et al have reported a positive correlation between serum DCA levels and proliferation measured by flow cytometric cell cycle analysis. However, a more recent study of colorectal adenoma patients failed to detect a correlation between serum DCA and immuno-histochemical Ki-67 antigen labelling. Instead, this latter study revealed a positive correlation between serum DCA and the degree of TUNEL-positive epithelial cell apoptosis. ... 

FDA) for use in humans to treat malaria because this drug is considered a safe drug with few side effects.These features prompted various scientists around the world to evaluate the potential of artemisinin (1) and derivatives to control cancer cells proliferation. This chapter reviews the recent advances on analytical methods for extraction and quantification of artemisinin (1) from A. annua. Examples of artemisinin-derivatives with antiproliferative activities are listed, describing the structure-activity relationships of 96 compounds. This knowledge is essential for future development and use of artemisinin derivatives in cancer therapy. The mechanism of action of artemisinin and derivatives on cancer cells have been well reviewed in literature and therefore is not discussed in this chapter. 

Biesterfeld, S., Striepecke, E., and Bocking, A. 1995. Single-cell-based quantification of the proliferation fraction by image analysis. Virchows Arch. 427 339-341. 

Numerous reviews have documented immune modulation in response to metal, pesticide and organic contaminants in fish4,19,37,135. Assays traditionally used to assess perturbations of immune function in fish fall into three broad categories pathogen challenge models, assays that monitor immune suppression/activation or immuno-pathology. Immune suppression/activation is usually measured with assays of phagocytosis, respiratory burst, cell proliferation, as well as quantification of soluble factors such as lysozyme, serum antibody, CRP or complement. 

Cell proliferation Kit (MTT Roche) colorimetric assay for non-radioactive quantification of cell viability and proliferation. 

The methods of FBA and elementary flux modes study interactions between different routes in a metabolic network and the quantification of flux distributions but do not evaluate how fluxes are controlled. In Metabolic Control Analysis (MCA), the control exerted by the rate of a reaction over a substrate flux or any other system parameter (e.g., metabolite concentration or cell proliferation) can be described quantitatively as a control coefficient. The control coefficient is a relative measure of how much a perturbation affects a system variable and is defined as the fractional change in the system property over the fractional change in the reaction rate [e.g., Bums et al. 1985],... 

Figure 8.8 Data on the CA-4-P inhibition of growth factor-induced endothelial cell proliferation and migration, (a) CA-4-P inhibition of HUVEC proliferation, (b) Absence SMCs sensitivity to CA-4-P. (c) Resistance of HUVECs to CA-4-P. (d) CA-4-P inhibition of HUVEC migration, (e) Quantification of recovery of each denuded area after CA-4-P treatment [75]. (Reproduced with permission from Elsevier.)...

Emanuels, A. G., Burger, M. P. M., Hollema, H., and Koudstaal, J. 1996. Quantification of proliferation-associated markers Ag-NOR and Ki-67 does not contribute to the prediction of lymph node metastases in squamous cell carcinoma of the vulva. Hum. Pathol. 27 807-811. 

Historically the functions of cytokines have been elucidated first with bioassays preceding immunoassays for cytokine quantification. The bioassay of a given cytokine is based on its bioactivity in a defined biological model, normally based on a certain cell line. Various approaches have been reported (1) proliferation tests— induction of cell growth (e.g., B9 cell line for IL-6) (2) tests for cytotoxicity— TNFa on WEHI164... 

Background Untreatable metastasis, rather than the primary tumor, is the cause of mortality in breast cancer. Myeloid-derived suppressor cells (MDSCs) are hema-topoetic cells that home specifically to the tumors and have a major role in tumor invasion and metastasis and the development of resistance to chemotherapy. MDSCs proliferate in response to tumors and accumulate in the spleen, from which they can be isolated using their Grl and CDllb surface markers. The objective was to use label-free mass spectrometry and shotgun proteomics to characterize MDSCs that associate with two mouse cell lines derived from the same tumor, one from the primary tumor (67NR) and the other from cells that have already metastasized to various organs (4T1). Spectral counting, for quantification, and protein network analysis were used to search for MDSC biomarkers characteristic to metastasis. 

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