Migration, cell, fibronectin

Deryugina El, Bourdon MA (1996) Tenascin mediates human glioma cell migration and modulates cell migration on fibronectin. J Cell Sci 109(Pt 3) 643-652... 

TGF-P also regulates cell-substratum interactions by a selectively increasing expression of integrins, a major class of cell-adhesion receptors. Integrins bind fibronectin, collagens, and other ECM proteins28-29 and thus modulate the adhesion cascade, which modulates cell migration. 

Boucaut, J.C., Darribere, T., Poole, T.J., Aoyama, H., Ya-mada, D.M. and Thiery, J.P. (1984) Biologically active peptides as probes of embryonic development a competitive peptide inhibitor of fibronectin function inhibits gastru-lation in amphibian embryos and neural crest cell migration in avian embryos. J. Cell Biol. 99 1822-1830. 

Ffrench-Constant, C. (1988) Patterns of fibronectin gene expression and splicing during cell migration in chicken embryos. Development 104 369-382. 

Perris, R. and Johansson, S. (1987) Amphibian neural crest cell migration on purified extracellular matrix components a chondroitin sulfate proteoglycan inhibits locomotion on fibronectin substrates. J. Cell Biol. 105 2511-2521. 

Perris, R., Pausson, M. and Bronner-Fraser, M. (1989) Molecular mechanisms of avian neural crest cell migration on fibronectin and laminin. Dev. Biol. 136 222-239. 

Rovasio, R.A., Delouvde, A., Yamada, K.M., TimpI, R. and Thiery, J.P. (1983) Neural crest cell migration requirements for exogenous fibronectin and high density. J Cell Biol. 96 462- 73. 

Husmann, K., Faissner, A. and Schachner, M. (1992) Tenas-cin promotes cerebellar granule cell migration and neurite outgrowth by different domains in the fibronectin type III repeats. J. Cell Biol. 116 1475-1486. 

Fig. 8. An electric field directs migration of a differentiated HL60 cell. Differentiated HL60 cells are cultured on fibronectin-coated glass slides and exposed to an electric field. The field direction is reversed after 15 min. The cell migration is directed by the field. The dotted line illustrates the trajectory of cell migration.

Adherent Cell Migration 1. Cell incubator. 2. Migration chambers from Neuro Probe Inc. 3. Collagen IV or fibronectin solution. 4. Crystal violet solution. 5. Serum-free medium supplemented with 0.1% BSA and 10 mM... 

It is well documented that N-formyl peptides induce a rapid, transient rise in intracellular calcium in neutrophils. It is also well established that inositol 1,4,5-trisphosphate released by the action of PLC triggers the release of Ca from intracellular stores. With the exception of the specific condition of neutrophils migrating on fibronectin and vitronectin [91], migration appears to occur in the absence of the intracellular calcium transient [196, 299, 456]. However, the studies that demonstrated this conclusion utilized indicators and buffers of cytosolic calcium that may not probe the entire calcium pool. Evidence suggests two additional mechanisms for regulating calcium that may have an impact on cell migration. 

Hyaluronan has also been used in the synthesis of biological scaffolds for wound-heaUng applications. These scaffolds typically have proteins such as fibronectin attached to the hyaluronan to facilitate cell migration into the... 

---