Parallel artificial-membrane permeability assay

The use of 96-well microtiter plates coupled with the rapid quantification by a spectrophotometric plate reader allows this system to screen a large array of compounds in a relatively short period of time. Alternatively, the compound analysis can be performed with fast and sensitive LC/MS by pooling the samples together. Both the spectrophotometric and mass spectrometric methods 

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Kansy, M., Senner, F., Gubemator, K. Physicochemical high throughput screening parallel artificial membrane permeability assay in the description of passive absorption processes. J. Med. Chem. 1998, 41, 1007-1010. 

BBB PAMPA Blood brain barrier parallel artificial membrane permeability assay... 

PARALLEL ARTIFICIAL-MEMBRANE PERMEABILITY ASSAY (PAMPA)... 

Zhu, C. Chen, T.-M. Hwang, K., A comparative study of parallel artificial membrane permeability assay for passive absorption screening, in CPS A2000 The Symposium on Chemical and Pharmaceutical Structure Analysis. Milestone Development Services. Princeton, NJ, Sept. 26-28, 2000. 

Sugano, K. Hamada, H. Machida, M. Ushio, H., High throughput prediction of oral absorption Improvement of the composition of the lipid solution used in parallel artificial membrane permeability assay, J. Biomolec. Screen. 6, 189-196 (2001). 

This book is written for the practicing pharmaceutical scientist involved in absorption-distribution-metabolism-excretion (ADME) measurements who needs to communicate with medicinal chemists persuasively, so that newly synthesized molecules will be more drug-like. ADME is all about a day in the life of a drug molecule (absorption, distribution, metabolism, and excretion). Specifically, this book attempts to describe the state of the art in measurement of ionization constants (p Ka), oil-water partition coefficients (log PI log D), solubility, and permeability (artificial phospholipid membrane barriers). Permeability is covered in considerable detail, based on a newly developed methodology known as parallel artificial membrane permeability assay (PAMPA). 

Kerns EH, Di L, Petusky S, Farris M, Ley R, Jupp P (2004) Combined application of parallel artificial membrane permeability assay and Caco-2 permeability assays in drug discovery. J Pharm Sci 93 1440-1453. 

Table 18.3 Parallel artificial membrane permeability assay (PAMPA) permeability and human absorption for 19 drug compounds.

Galinis-Luciani, D., Nguyen, L. and Yazdanian, M. (2007) Is parallel artificial membrane permeability assay a useful tool for discovery Journal of Pharmaceutical Sciences, 96, 2886-2892. 

Avdeef, A., Bendels, S., Di, L., Fallen B., Kansy, M., Sugano, K. and Yamauchi, Y. (2007) Parallel artificial membrane permeability assay (PAMPA)-critical factors for better predictions of absorption. Journal of Pharmaceutical Sciences, 96, 2893-2909. 

A novel design of artificial membrane for improving the parallel artificial membrane permeability assay model. Pharmaceutical Research, 25, 1511-1520. 

A novel application of the parallel artificial membrane permeability assay (PAM-PA) system to measuring protein binding constants was demonstrated recently [41]. 

Abbreviations ADME, Adsorption distribution metabolism excretion CSF, Cerebrospinal fluid PAMPA, Parallel artificial membrane permeability assay. 

To predict in vivo permeabilities, the parallel artificial membrane permeability assay (PAMPA) was introduced in 1998. Since then, it has gathered considerable interest in the pharmaceutical industry [41-47]. This method uses a phospholipid-coated filter separating two aqueous compartments to mimic the passive transport of small molecules. It readily provides information about passive-transport perme-... 

II.E.3 Parallel Artificial Membrane Permeability Assay (PAMPA) 471... 

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