Carbohydrate-binding specificity, of lectins

Yamamoto K, Ito S, Yasukawa F, Konami Y, Matsumoto N. Measurement of the carbohydrate-binding specificity of lectins by a multiplexed bead-based flow c)4ometric assay. Anal. Biochem. 2005 336 28-38. 

Kornfeld, K., Reitman, M.-L. and Kornfeld, R. (1981). The carbohydrate-binding specificity of pea and lentil lectins. Fucose is an important determinant. Journal of Biological Chemistry, 256,6633-6640. 

Studies on the carbohydrate-binding specificity of the fava-bean lectin, as determined by hapten inhibition of hemagglutination,140,213,466-469 showed that the lectin is inhibited by Makela s group III sugars (see Table VI). Hemagglutination by the lectin was inhibited by... 

Phenyl and p-nitrophenyl /3-D-galactopyranoside, as well as lactose, were poorly inhibitory.19,478 The high lectin-reactivity reported for 2 -O-L-fucosyllactose is interesting, but inconsistent with the observed carbohydrate-binding specificity of the lectin (the authors might well have tested the very active trisaccharide just noted).196... 

The carbohydrate-binding specificity of purified Dolichos biflorus lectin has been studied in detail by Etzler and Kabat.108 In precipitin studies conducted on the Dolichos lectin, 87-99% was precipitated by ovarian cyst A substance, whereas only 60-70% was precipitated by hog A substance the latter result was attributed to the formation of soluble, lectin-oligosaccharide complexes. At substances were more reactive than A2 substances however, no reaction was observed with B or O substances, even following mild hydrolysis with acid or two stepwise, Smith degradations. N-Deacetylation of A substance with... 

Extensive information on the carbohydrate-binding specificity of the Helix pomatia lectin was obtained by examining the extent to which a large number of sugars inhibited the precipitation reaction between agglutinin and human blood-group A substance or Salmonella typhinurium SH 180 lipopolysaccharide83,178,581,582 (see Table XI). 

A comparative study of the carbohydrate-binding specificity of four 2-acetamido-2-deoxy-D-galactose-binding lectins (from Dolichos biflorus, Glycine max, Helix pomatia, and Phaseolus lunatus) has been conducted by using a series of model macromolecules for direct precipitation and a variety of mono- and oligo-saccharides as hapten inhibitors.592 The data indicated that the combining site of all four lectins... 

Figs. 14 to 16 present the structures of a series of naturally occurring glycopeptides, and summarize the carbohydrate-binding specificity of a number of lectins in terms of the carbohydrate units with which they interact. 

Table 3 presents the carbohydrate-binding specificity of several monocotyledonous bulb lectins. In all cases, specificity is directed toward mannose oligosaccharides. 

The carbohydrate-binding specificity of the lima bean lectin studied by inhibition of precipitation, and a sulfhydryl group protection assay [161], revealed the type A trisaccharide GalNAc(al-3)[L-Fuc(al-2)]Gal to be the best inhibitor, 40 times more potent than GalNAc. The a-glycosides of GalNAc are 8 times more potent than the corresponding 3-anomers. 

Firon, N., Ofek, I., and Sharon, N. (1984). Carbohydrate-binding sites of the mannose-specific fimbrial lectins of enterobacteria. Infect. Immun. 43,1088-1090. 

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