Carbohydrate binding

Houseman BT, Mrksich M (2002) Model Systems for Studying Polyvalent Carbohydrate Binding Interactions. 218 1-44 Hricovlniovd Z, see PetruS L (2001) 215 15-41... 

Lectins are carbohydrate-binding proteins involved in cell adhesion and other biologic processes. 

There is one or more extended hydrophilic stretch in the hydrophobic domain which may be associated with carbohydrate binding. 

Crude chloroform-methanol-water (30 60 8, v/v) extracts of immunostainedTLC bands were analyzed without further purification by nanoelectrospray low-energy mass spectrometry. The authors showed that this effective PLC/MS-joined procedure offers a wide range of applications for any carbohydrate-binding agents such as bacterial toxins, plant lectins, and others. Phenyl-boronic acid (PBA) immobilized on stationary support phases can be put to similar applications. This technology, named boronate affinity chromatography (BAC), consists of a chemical reaction of 1,2- and 1,3-diols with the bonded-phase PBA to form a stable... 

Several carbohydrate-binding proteins have been isolated from Sambucus nigra and two haemagglutinins have been isolated from its bark. Its seeds contain a lectin that is related to the immunological properties of the bark. One of its carbohydrate-binding proteins, called nigrin, is made of two subunits and is reported to cause red blood cells to clump. 5... 

All have an N-terminal predicted signal sequence of 18 aa, with mature proteins starting at amino acid 19. Other numbers above the bars are those of the last amino acid in each domain. The key carbohydrate-binding residues (QPD or EPD) are shown, as is the position of the unusual double cysteine in the adjacent loop. Solid circles represent A/-glycosylation sites. 

Such sugar-dendrimer complexes ( sugar balls ) have been used to inhibit the interactions of viruses with cell surfaces. Many viruses bind to particular carbohydrate residues on cell surfaces, which in turn facilitate their entry into cells and the resultant infection process. A virus particle presents a multi-dentate surface consisting of many carbohydrate-binding proteins able to interact with multiple cell-surface carbohydrates. The surface of a dendrimer that is modified with... 

Figure 7.13 Lactone sugar derivatives can be used to react with amine-containing dendrimers, which results in coupling via amide bonds. The sugar ball dendrimers then can be used to specifically bind to carbohydrate binding proteins.

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