Of lectins

Fishman, C.W., Udey, M.C., Kurtz, M. and Wedner, J.J. (1981). Inhibition of lectin-induced lymphocyte activation by 2-cyclohexen-l-one decreased intracellular glutathione inhibits an early event in the activation sequence. J. Immunol. 127, 2257-2262. 

This process seems to reflect gradual increases in the intensity and density of labelled receptor cell bodies and their axons, followed by regional bulbar staining as synaptogenesis proceeds. However, a more extensive range of lectins needs to be examined before the implications of species differences in the membrane glycoproteins can be satisfactorily interpreted (Salazar and Quinteiro, 1998). 

Crosslinking of Lectins with the Mucins Correlate with Decreasing Favorable Entropy of... 

While the mechanisms of formation of noncovalent crosslinked lattices of lectins with multivalent carbohydrates and glycoproteins have been well investigated,15-17 the mechanisms associated with the enhanced affinities of lectins binding to multivalent carbohydrates and glycoproteins have been less well investigated until recently.18,19... 

Implications of Increasing Negative Cooperativity and Decreasing Favorable Binding Entropy of Lectins-Mucin Crosslinking Interactions... 

T. K. Dam and C. F. Brewer, Thermodynamic studies of lectin-carbohydrate interactions by isothermal titration calorimetry, Chem. Rev., 102 (2002) 387 -29. 

S. Andre, B. Liu, H.-J. Gabius, and R. Roy, First demonstration of differential inhibition of lectin binding by synthetic tri- and tetravalent glycoclusters from cross-coupling of rigidified 2-propynyl lactoside, Org. Biomol. Chem., 1 (2001) 3909-3916. 

Reported applications of SADP include the crosslinking of con A to receptors oVn human erythrocyte membranes (Vanin and Ji, 1981), site-specific labeling of lectins using modified... 

Figure 22.15 Glycolipid components included in liposome construction may be used to couple antibody molecules by using conjugates of lectins with the proper specificity for binding the sugar groups.

The following generalized protocol is an adaptation for the labeling of 15 nm gold particles with Aplysia gonad lectin, as described by Benhamou et al., 1988. Each lectin-gold preparation will have its own unique pH optimum and ratio of lectin-to-gold for the absorption process. 

Babu, P., Sinha, S., and Surolia, A. (2007) Sugar-quantum dot conjugates for a selective and sensitive detection of lectins. Bioconjugate Chem. 18, 146-151. 

Baenzigen J.U., and Fiete, D. (1982) Photoactivatable glycopeptide reagents for site-specific labeling of lectins./. Biol. Chem. 257, 4421M425. 

Moroney, S.E., D Alarcao, L.J., Goldmacher, V.S., Lambert, H.M., and Blattlcr, W.A. (1987) Modification of the binding site(s) of lectins by an affinity column carrying an activated galactose-terminated ligand. Biochemistry 26, 8390. 

Nicolson, G.L. (1978) Ultrastructural localization of lectin receptors. In Advanced Techniques in Biological Electron Microscopy (M. Koehler, ed.), p. 1. Springer-Verlag, New York. 

Roth, J. (1983) Application of lectin—Gold complexes for electron microscopic localization of glycoconjugates on thin sections./. Histochem. Cytochem. 31, 987. 

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