Dolichos biflorus lectin

Cuatrecasas (45). The Dolichos biflorus lectin was a gift from Dr. Marilynn Etzler (46). Ulex europeus and Bandeirea simplicifolia lectins were purified in our laboratory and iodinated with Nal25i (carrier-free) by Sepharose 4B-bound lactoperoxidase (47)and diazotized iodoaniline coupling (48) procedures, respectively. 

The specific activities of I-labeled Dolichos biflorus lectin and cholera toxin were 1.12 x 108 cpm and 3.1 x 10 cpm per mg of protein, respectively. 

An alternative purification of Dolichos biflorus lectin was published by Font and coworkers.518 Fractional precipitation with ammonium... 

The carbohydrate-binding specificity of purified Dolichos biflorus lectin has been studied in detail by Etzler and Kabat.108 In precipitin studies conducted on the Dolichos lectin, 87-99% was precipitated by ovarian cyst A substance, whereas only 60-70% was precipitated by hog A substance the latter result was attributed to the formation of soluble, lectin-oligosaccharide complexes. At substances were more reactive than A2 substances however, no reaction was observed with B or O substances, even following mild hydrolysis with acid or two stepwise, Smith degradations. N-Deacetylation of A substance with... 

Characterization of the protein glycosylation of Dolichos biflorus lectin was reported by Wuhrer et al. [88]. The protein was digested by means of unspecific... 

A lectin, isolated from the seeds of Amphicarpaea bracteata by affinity chromatography on 2-acetamido-2-deoxy-D-galactose coupled to epoxy-activated agarose, has been shown to have characteristics common with Dolichos biflorus lectin, especially in relation to the agglutination of human Ai erythrocytes. It is assumed that the lectin interacts with non-reducing terminal 2-acetamido-2-deoxy-D-galactosyl residues on the red cell membrane of these erythrocytes. 

Dolichos biflorus Lectin (DEL) Forssman disaccharide ILUl [115]... 

Circular dichroism (CD) has been utilized to investigate the effect of saccharides on the conformation of lectins in solution. CD has demonstrated saccharide-induced conformational changes in the lectins from Canavalia ensiformis (7), Dolichos biflorus (8), Ricinus communis (9), and Triticum vulgaris (10). The present study uses circular dichroism to assess the secondary structure of SBA and to measure conformational transitions induced by the saccharides which bind to this lectin. These and previous studies will contribute to a clearer understanding of the unique properties of these sugar-binding proteins. 

A comparative study of the carbohydrate-binding specificity of four 2-acetamido-2-deoxy-D-galactose-binding lectins (from Dolichos biflorus, Glycine max, Helix pomatia, and Phaseolus lunatus) has been conducted by using a series of model macromolecules for direct precipitation and a variety of mono- and oligo-saccharides as hapten inhibitors.592 The data indicated that the combining site of all four lectins... 

Lectin from Dolichos biflorus jV-Acetyl-D-galactosamine Spheron 84... 

M.E., Two lectin genes differentially expressed in Dolichos biflorus differ primarily by a 116-base pair sequence in their 5 flanking regions, J. Biol Chem., 1990, 265, 4997-5001. 

BUNKER, T.W., ETZLER, M.E., The stem and leaf lectin of Dolichos biflorus L., previously thought to be cell wall associated, is sequestered in vacuoles, Planta, 1994,192, 144-147. 

Removal of 40% of the D-mannosyl residues of the lectin from Dolichos biflorus by digestion with a-D-mannosidase does not affect the agglutination of type A erythrocytes or the precipitation of blood group A and H substances, suggesting that complete integrity of the carbohydrate components of the lectin is not necessary for biological activity. ... 

A number of lectins have been purified by affinity chromatography on 0-glycosyl polyacrylamide gels. The physicochemical properties of well-characterized lectins such as Glycine soja, Dolichos biflorus, Phaseolus lunatus. Helix pomatia, and Ricinus communis lectins compared well with those of preparations obtained by alternative chromatographic systems. 

Study of periodate oxidation or a-o-mannosidase treatment of the phyto-haemagglutinin from Dolichos biflorus suggests that complete integrity of the carbohydrate unit is not essential for biological activity. A 16% loss of D-mannose, on periodate treatment, did not affect the lectin s ability to agglutin-... 

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