Ulex europeus

Figure 4. Pea stem material was sequentially extracted with hot 70% ethanol, 0.1 M EDTA and 4% KOH-0.1% NaBH4 to leave xyloglucan-cellulose cell wall ghosts . Binding of fluorescent fucose-binding lectin from Ulex europeus as visualized by fluorescence microscopy shows xyloglucan distributed over the whole wall surface. Photograph courtesy of Dr. T. Hayashi.

Cell division is stimulated by lectins (for a review, see Ref. 188) and by other saccharide-directed reagents, for example, sodium periodate and D-galactose oxidase after desialylation,189 but there is no evidence that it is the fucopyranosyl residues that are the specific receptors in these instances. The L-fucose-specific lectins from, for example, Lotus tetragonolobus and Ulex europeus are not mitogenic. 

Cuatrecasas (45). The Dolichos biflorus lectin was a gift from Dr. Marilynn Etzler (46). Ulex europeus and Bandeirea simplicifolia lectins were purified in our laboratory and iodinated with Nal25i (carrier-free) by Sepharose 4B-bound lactoperoxidase (47)and diazotized iodoaniline coupling (48) procedures, respectively. 

Ulex Europeus [ I]Lectin Binding to Neuroblastoma Cell Surfaces... 

The specific activity of Ulex europeus [125I]lectin was 0. 5 x 10 cpm per mg of... 

Characterization of the carbohydrate-binding specificity of Ulex europeus II is still very rudimentary. Studies by inhibition of hemagglutination have been completed, using only a few of the appropriate sugars necessary to define specificity of the binding. 

Fig. 15.—Human IgA, Glycopeptide871 Showing the Carbohydrate-binding Loci for Various Lectins, [a. Limulus polyphemus b, Triticum vulgaris c, Ricinus communis d, Sophora japonica e, Abrus precatorius f, Cytisus sessilifolius g, Phaseolus vulgaris-, h, Canavalia ensiformis i, Lens culinaris j, Pisum sativus k, Vicia faba-, 1, Bandeiraea simplicifolia II m, Solanum tuberosum and n, Ulex europeus II.]...

Ulex europeus agglutinin UEAl 170000 a-L-Fucose 25 mM Fucose... 

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