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Capillary serum

Blood obtained by skin puncture is contaminated to some extent with interstitial and intracellular fluids. The major differences between venous serum and capillary serum are illustrated in Table 2-4. [Pg.48]

Analogous to two-dimensional LC, the on-line coupling of LC and CE has been carried out both in the heart-cut and the comprehensive mode. In a heart-cut LC-CE study, a protein G immunoaffinity LC column was used to selectively preconcentrate insulin from serum (171). A 1 p.1 elution plug comprising the insulin was switched on-line to the CE system where a part was injected into the capillary for final separation. With CE, efficient separations can be obtained in a... [Pg.289]

Sanq>ling With Capillaries. Earlier it was pointed out that with the aid of a constriction pipette a sample can be measured accurately. A similar effect can be obtained by allows ing the serum to enter a capillary open at both ends. The serum will reach the opposite end of the capillary if it is held at an angle and then stoppered, and if the capillary has been carefully calibrated an accurate volume can be measured out. Such capillaries are now available commercially, and have been in use in automated equipment in the authors laboratory for at least 10 years. It is now possible to take the capillary and empty its contents into a container for analysis, or into a stream for the purpose of determining any of the materials which can be determined with the autoanalyzer. Figure 33 shows an instrument which is used for this purpose (58). [Pg.138]

Oda RP, Clark R, Katzman JA, Landers JP (1997) Capillary electrophoresis as a clinical tool for the analysis of protein in serum and other body fluids. Electrophoresis 18 1715-1723. [Pg.233]

Kilar, E and Hjerten,S., Fast and high resolution analysis of human serum transferrin by high performance isoelectric focusing in capillaries, Electrophoresis, 10, 23, 1989. [Pg.420]

Kilar, F. and Fanali, S., Separation of tryptophan-derivative enantiomers with iron-free human serum transferrin by capillary zone electrophoresis, Electrophoresis, 16, 1510, 1995. [Pg.421]

Thormartn, W., Meier, R, Marcolli, C., and Binder, F., Analysis of barbiturates in human serum and urine by high-performance capillary electrophoresis-micellar electrokinetic capillary chromatography with on-column multiwavelength detection, /. Chromatogr., 545, 445, 1991. [Pg.423]

Heegard, N. H. H. and Robey, R A., Use of capillary zone electrophoresis to evaluate the binding of anionic carbohydrates to synthetic peptides derived from human serum amyloid P component, Anal. Chem., 64, 2479, 1992. [Pg.426]

The pathophysiologic mechanisms of portal hypertension and of cirrhosis itself are entwined with the mechanisms of ascites (Fig. 19-3). Cirrhotic changes and the subsequent decrease in synthetic function lead to a decrease in production of albumin (hypoalbuminemia). Albumin is the major intravascular protein involved in maintaining oncotic pressure in the vascular system low serum albumin levels and increased capillary permeability allow fluid to leak from the vascular space into body tissues. This can result in peripheral edema, ascites, and fluid in the pulmonary system. The obstruction of hepatic sinusoids and... [Pg.326]

The lymphatic system of the skin extends up and into the papillary layers of the dermis. A dense, flat meshwork of lymphatic capillaries is found here [11]. Lymph passes into a deeper network at the lower boundary of the dermis. Serum, macrophages, and lymphocytes readily negotiate through the skin s lymphatic and vascular networks. [Pg.198]

Although most drugs are absorbed from the intestine by the blood capillary network in the villi, they can also be taken up by the lymphatic system (an integral and necessary part of the vascular system, the function of which is to collect extra tissue fluid and return it to the vascular compartment), particularly by M cells that reside in the Peyer s patch regions of the intestine. Peyer s patches have also been implicated in the regulation of the secretory immune response. Wachsmann et al. [277] reported that an antigenic material encapsulated within a liposome, when administered perorally, is taken up by these M cells and exhibited better saliva and serum IgA (primary and secondary)... [Pg.578]

AC ADME ANS AUC BA/BE BBB BBM BBLM BCS BLM BSA CE CHO CMC CPC CPZ CTAB CV DA DOPC DPPC DPPH aminocoumarin absorption, distribution, metabolism, excretion anilinonaphthalenesulfonic acid area under the curve bioavailability-bioequivalence blood-brain barrier brush-border membrane brush-border lipid membrane biopharmaceutics classification system black lipid membrane bovine serum albumin capillary electrophoresis caroboxaldehyde critical micelle concentration centrifugal partition chromatography chlorpromazine cetyltrimethylammonium bromide cyclic votammetry dodecylcarboxylic acid dioleylphosphatidylcholine dipalmitoylphosphatidylcholine diphenylpicrylhydrazyl... [Pg.304]

Valproic acid has been determined in human serum using capillary electrophoresis and indirect laser induced fluorescence detection [26], The extract is injected at 75 mbar for 0.05 min onto a capillary column (74.4 cm x 50 pm i.d., effective length 56.2 cm). The optimized buffer 2.5 mM borate/phosphate of pH 8.4 with 6 pL fluorescein to generate the background signal. Separation was carried out at 30 kV and indirect fluorescence detection was achieved at 488/529 nm. A linear calibration was found in the range 4.5 144 pg/mL (0 = 0.9947) and detection and quantitation limits were 0.9 and 3.0 pg/mL. Polonski et al. [27] described a capillary isotache-phoresis method for sodium valproate in blood. The sample was injected into a column of an EKI 02 instrument for separation. The instrument incorporated a conductimetric detector. The mobile phase was 0.01 M histidine containing 0.1% methylhydroxycellulose at pH 5.5. The detection limit was 2 pg/mL. [Pg.230]

It is important, however, to remove the protein layer from the surface over which the air/liquid meniscus is displaced during the measurement so as to assure that the conditions of flow with and without the layer in the capillary are totally comparable. Using this method we find that in the case of bovine serum albumin very thick layers are formed layers whose thickness grows in direct proportionality to albumin concentration up to 15% w/v, at least. We also find a reversible doubling of layer size as temperature is raised from 7.7 to 15°C in the case of triple helical soluble collagen adsorbed end on to glass. [Pg.161]

Consequently, the research work of Hara s group continued focusing on the improvement of protein determination using CE combined with online CL detection. By replacing EY by the Rhodamine B isothiocyanate (RITC) dye in the binary complexes formed with the proteins BSA or human serum albumin (HSA) and using a different imidazole buffer solution of pH 6, the sensitivity was increased [72], However, best detection limits for these determinations were found employing the tetramethylrhodamine isothiocyanate isomer (TRITC) dye, left for 4 h with a standard solution of BSA in acetonitrile followed by introduction into the capillary. For BSA, a detection limit of 6 nM was reached [73],... [Pg.441]

Several methods for measuring drug binding to human serum albumin involving the determination of retention times on HPLC columns with bound albumin have been reported [77,78]. Solid-phase microextraction [79,80], capillary electrophoresis [81], and displacement of near-infrared fluorescent labels [82] have all been studied. [Pg.499]


See other pages where Capillary serum is mentioned: [Pg.196]    [Pg.265]    [Pg.266]    [Pg.299]    [Pg.67]    [Pg.330]    [Pg.604]    [Pg.138]    [Pg.78]    [Pg.421]    [Pg.377]    [Pg.411]    [Pg.425]    [Pg.433]    [Pg.435]    [Pg.371]    [Pg.1560]    [Pg.358]    [Pg.39]    [Pg.367]    [Pg.169]    [Pg.215]    [Pg.234]    [Pg.234]    [Pg.79]    [Pg.596]    [Pg.90]    [Pg.375]    [Pg.375]    [Pg.227]    [Pg.236]    [Pg.600]    [Pg.30]    [Pg.31]   
See also in sourсe #XX -- [ Pg.48 , Pg.48 ]




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