Centrifugal partition chromatography

Actual droplet formation is not necessary as long as the mobile phase can pass through the stationary phase without displacing it. As a matter of fact, both aqueous and nonaqueous biphasic systems have been used (51, 332). Recent results reveal that a variety of natural products such as saponins, saturated fatty acids, and biomacromolecules - the latter on a biphasic aqueous polymer system - can be separated (332). Because of its comparatively rapid and non-destructive action, CPC has recently been used in the isolation of the tunichromes, the rather sensitive phenolic peptides from the vanadium-collecting sea squirt Ascidia nigra (53, 54) and of W-cis- and trans-rciindA from a complex photoisomerization mixture (51). 

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A. P. Eoueault (Ed.), Centrifugal Partition Chromatography, Chromatographie Seienee Series, Vol. 68, Mareel Dekker, New York (1995)... 

Centrifugal Partition Chromatography, edited by Alain P Foucault... 

R. -S. Tsai, P.-A. Carrupt, and B. Testa, Measurement of Partition Coefficients Using Centrifugal Partition Chromatography. Method Development and Application to the Determination of Solute Properties, American Chemical Society, Washington DC, 1995, pp. 143-154. 

Centrifugal partition chromatography (CPC) has been used to characterize the partitioning behavior of hydrophilic molecules, where log D values as low as —3 can be obtained [371,377-379]. It is not as popular a method as it used to be, apparently due to instrumental challenges. Cyclic voltammetry (CV) has become the new method used to get access to very low log D values, with partition coefficients reported as low as —9.8 [261,269,362]. 

El Tayar, N. Tsai, R.-S. Carrupt, P.-A. Testa, B., Octan-l-ol-water partition coefficinets of zwitterionic a-amino acids. Determination by centrifugal partition chromatography and factorization into steric/hydrophobic and polar components, J. Chem. Soc. Perkin Trans. 2, 79-84 (1992). 

AC ADME ANS AUC BA/BE BBB BBM BBLM BCS BLM BSA CE CHO CMC CPC CPZ CTAB CV DA DOPC DPPC DPPH aminocoumarin absorption, distribution, metabolism, excretion anilinonaphthalenesulfonic acid area under the curve bioavailability-bioequivalence blood-brain barrier brush-border membrane brush-border lipid membrane biopharmaceutics classification system black lipid membrane bovine serum albumin capillary electrophoresis caroboxaldehyde critical micelle concentration centrifugal partition chromatography chlorpromazine cetyltrimethylammonium bromide cyclic votammetry dodecylcarboxylic acid dioleylphosphatidylcholine dipalmitoylphosphatidylcholine diphenylpicrylhydrazyl... 

Gluck, S.J., Martin, E.J. (1990) Extended octanol-water partition coefficient determination by dual-mode centrifugal partition chromatography. J. Liq. Chromatogr. 13, 3559-3570. 

Menges, R.A., Bertrand, G.L., Armstrong, D.W. (1990) Direct measurement of octanol-water partition coefficients using centrifugal partition chromatography with a back-flushing technique. J. Liq. Chromatogr. 13, 3061-3077. 

Berthod, A., Han, Y.I., and Armstrong, D.W. Centrifugal partition chromatography. V. Octanol-water partition coefficients, direct and indirect determination, J. Llq. Chromatogr., 11 1441-1456, 1988. 

A number of techniques have been used for the preparative separation of flavonoids. These include HPLC, Diaion, Amberlite XAD-2 and XAD-7, and Fractogel TSK/Toyopearl HW-40 resins, gel filtration on Sephadex, and centrifugal partition chromatography (CPC). The choice of methods and strategies varies from research group to research group and depends often on the class of flavonoid studied. 

Marston, A., Slacanin, 1., and Hostettmann, K. Centrifugal partition chromatography in the separation of natural products, Phytochem. Anal, 1, 3, 1990. 

Centrifugal partition chromatography (CPC) relies on centrifugal force rather than gravitation for the retention of the stationary phase and solvents can be pumped at higher speeds through the instruments. In addition, no need for droplets formation is required. This allows shorter separation times, without loss of resolution, and an infinite choice of solvents with the only requirement of forming two immiscible phases, stable with the time. Chloroform-based systems have been mostly used for the separation of saponins due to their favourable partition coefficient towards such solvents. [116, 119, 120]. 

Foiicaulr, A.P., Editor Centrifugal Partition Chromatography, Maecel Dekker. Tnc, New York, NY, 1995... 

ACJ Hermans-Lokkerbol, R Verpoorte. Preparative separation and isolation of three bitter a-bitter acids from hop, Humulus lupulus L., by centrifugal partition chromatography. J Chromatogr A 664 45-53, 1994. 

Centrifugal Partition Chromatography (CPC) has been shown to yield partition coefficients which compare quite favorably with those measured by traditional shake-flask (El Tayar, 1993). The procedure, which employs a coil planet type centrifuge in a horizontal flow-through system, has been described (El Tayar, 1991) The equipment can handle organic solvents with low viscosity (such as heptane) more readily than it handles octanol, but log octanol/water values in the range of -3.0 to +3.0 seem reliable. It has been used quite effectively in measuring the log Ps of amino adds (Tsai, 1991). 

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